Cancer-associated mutations in VAV1 trigger variegated signaling outputs and T-cell lymphomagenesis

[EN]Mutations in VAV1, a gene that encodes a multifunctional protein important for lymphocytes, are found at different frequencies in peripheral T-cell lymphoma (PTCL), non-small cell lung cancer, and other tumors. However, their pathobiological significance remains unsettled. After cataloguing 51 c...

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Detalles Bibliográficos
Autores: Robles Valero, Javier, Fernández‐Nevado, Lucía, Lorenzo Martín, L. Francisco, Fernández‐Pisonero, Isabel, Rodríguez‐Fdez, Sonia, Astorga‐Simón, Elsa N
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Universidad de Salamanca (USAL)
Repositorio:GREDOS. Repositorio Institucional de la Universidad de Salamanca
OAI Identifier:oai:gredos.usal.es:10366/168546
Acceso en línea:http://hdl.handle.net/10366/168546
Access Level:acceso abierto
Palabra clave:Angioimmunoblastic T-cell lymphoma
Follicular helper T cells
Oncogene
Peripheral T-cell lymphoma
Tumor suppressor
GTPases
Lymphoma
Animals
Humans
CD4-Positive T-Lymphocytes
rac1 GTP-Binding Protein
Cell Proliferation
COS Cells
3201.01 Oncología
linfoma
linfocitos T CD4-positivos
animales
humanos
proteína de unión al GTP rac1
proliferación celular
células COS
Descripción
Sumario:[EN]Mutations in VAV1, a gene that encodes a multifunctional protein important for lymphocytes, are found at different frequencies in peripheral T-cell lymphoma (PTCL), non-small cell lung cancer, and other tumors. However, their pathobiological significance remains unsettled. After cataloguing 51 cancer-associated VAV1 mutations, we show here that they can be classified in five subtypes according to functional impact on the three main VAV1 signaling branches, GEF-dependent activation of RAC1, GEF-independent adaptor-like, and tumor suppressor functions. These mutations target new and previously established regulatory layers of the protein, leading to quantitative and qualitative changes in VAV1 signaling output. We also demonstrate that the most frequent VAV1 mutant subtype drives PTCL formation in mice. This process requires the concurrent engagement of two downstream signaling branches that promote the chronic activation and transformation of follicular helper T cells. Collectively, these data reveal the genetic constraints associated with the lymphomagenic potential of VAV1 mutant subsets, similarities with other PTCL driver genes, and potential therapeutic vulnerabilities.