On-Growth and In-Growth Osseointegration Enhancement in PM Porous Ti-Scaffolds by Two Different Bioactivation Strategies

A lack of primary stability and osteointegration in metallic implants may result in implant loosening and failure. Adding porosity to metallic implants reduces the stress shielding effect and improves implant performance, allowing the surrounding bone tissue to grow into the scaffold. However, a bio...

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Autores: Rappe, Katrin Steffanie, Ortiz-Hernandez, Monica|||0000-0001-8875-9131, Punset, Miquel|||0000-0002-1904-8667, Molmeneu, Meritxell|||0000-0002-7181-6467, Barba, Albert, Mas-Moruno, Carles|||0000-0001-8337-0872, Guillem-Marti, Jordi|||0000-0003-0307-2221, Caparrós, Cristina, Rupérez, Elisa|||0000-0001-8845-512X, Calero, José, Manzanares, María-Cristina|||0000-0002-4585-4953, Gil Mur, Javier|||0000-0002-6824-1412, Franch, Jordi|||0000-0002-3731-8552
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:256059
Acceso en línea:https://ddd.uab.cat/record/256059
https://dx.doi.org/urn:doi:10.3390/ijms23031750
Access Level:acceso abierto
Palabra clave:Titanium foams
Osseointegration
Thermochemical treatment
RGD peptide
In vivo implantation
Histomorphometric evaluation
Bone on-growth
Bone in-growth
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spelling On-Growth and In-Growth Osseointegration Enhancement in PM Porous Ti-Scaffolds by Two Different Bioactivation StrategiesAlkali Thermochemical Treatment and RGD Peptide CoatingRappe, Katrin SteffanieOrtiz-Hernandez, Monica|||0000-0001-8875-9131Punset, Miquel|||0000-0002-1904-8667Molmeneu, Meritxell|||0000-0002-7181-6467Barba, AlbertMas-Moruno, Carles|||0000-0001-8337-0872Guillem-Marti, Jordi|||0000-0003-0307-2221Caparrós, CristinaRupérez, Elisa|||0000-0001-8845-512XCalero, JoséManzanares, María-Cristina|||0000-0002-4585-4953Gil Mur, Javier|||0000-0002-6824-1412Franch, Jordi|||0000-0002-3731-8552Titanium foamsOsseointegrationThermochemical treatmentRGD peptideIn vivo implantationHistomorphometric evaluationBone on-growthBone in-growthA lack of primary stability and osteointegration in metallic implants may result in implant loosening and failure. Adding porosity to metallic implants reduces the stress shielding effect and improves implant performance, allowing the surrounding bone tissue to grow into the scaffold. However, a bioactive surface is needed to stimulate implant osteointegration and improve mechanical stability. In this study, porous titanium implants were produced via powder sintering to create different porous diameters and open interconnectivity. Two strategies were used to generate a bioactive surface on the metallic foams: (1) an inorganic alkali thermochemical treatment, (2) grafting a cell adhesive tripeptide (RGD). RGD peptides exhibit an affinity for integrins expressed by osteoblasts, and have been reported to improve osteoblast adhesion, whereas the thermochemical treatment is known to improve titanium implant osseointegration upon implantation. Bioactivated scaffolds and control samples were implanted into the tibiae of rabbits to analyze the effect of these two strategies in vivo regarding bone tissue regeneration through interconnected porosity. Histomorphometric evaluation was performed at 4 and 12 weeks after implantation. Bone-to-implant contact (BIC) and bone in-growth and on-growth were evaluated in different regions of interest (ROIs) inside and outside the implant. The results of this study show that after a long-term postoperative period, the RGD-coated samples presented higher quantification values of quantified newly formed bone tissue in the implant's outer area. However, the total analyzed bone in-growth was observed to be slightly greater in the scaffolds treated with alkali thermochemical treatment. These results suggest that both strategies contribute to enhancing porous metallic implant stability and osteointegration, and a combination of both strategies might be worth pursuing. 22022-01-0120222022-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/256059https://dx.doi.org/urn:doi:10.3390/ijms23031750reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengAgencia Estatal de Investigación https://doi.org/10.13039/501100011033 RTI2018-098075-B-C21Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 RTI2018-098075-BC22Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2020-114019RB-I00open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2560592026-06-06T12:50:31Z
dc.title.none.fl_str_mv On-Growth and In-Growth Osseointegration Enhancement in PM Porous Ti-Scaffolds by Two Different Bioactivation Strategies
Alkali Thermochemical Treatment and RGD Peptide Coating
title On-Growth and In-Growth Osseointegration Enhancement in PM Porous Ti-Scaffolds by Two Different Bioactivation Strategies
spellingShingle On-Growth and In-Growth Osseointegration Enhancement in PM Porous Ti-Scaffolds by Two Different Bioactivation Strategies
Rappe, Katrin Steffanie
Titanium foams
Osseointegration
Thermochemical treatment
RGD peptide
In vivo implantation
Histomorphometric evaluation
Bone on-growth
Bone in-growth
title_short On-Growth and In-Growth Osseointegration Enhancement in PM Porous Ti-Scaffolds by Two Different Bioactivation Strategies
title_full On-Growth and In-Growth Osseointegration Enhancement in PM Porous Ti-Scaffolds by Two Different Bioactivation Strategies
title_fullStr On-Growth and In-Growth Osseointegration Enhancement in PM Porous Ti-Scaffolds by Two Different Bioactivation Strategies
title_full_unstemmed On-Growth and In-Growth Osseointegration Enhancement in PM Porous Ti-Scaffolds by Two Different Bioactivation Strategies
title_sort On-Growth and In-Growth Osseointegration Enhancement in PM Porous Ti-Scaffolds by Two Different Bioactivation Strategies
dc.creator.none.fl_str_mv Rappe, Katrin Steffanie
Ortiz-Hernandez, Monica|||0000-0001-8875-9131
Punset, Miquel|||0000-0002-1904-8667
Molmeneu, Meritxell|||0000-0002-7181-6467
Barba, Albert
Mas-Moruno, Carles|||0000-0001-8337-0872
Guillem-Marti, Jordi|||0000-0003-0307-2221
Caparrós, Cristina
Rupérez, Elisa|||0000-0001-8845-512X
Calero, José
Manzanares, María-Cristina|||0000-0002-4585-4953
Gil Mur, Javier|||0000-0002-6824-1412
Franch, Jordi|||0000-0002-3731-8552
author Rappe, Katrin Steffanie
author_facet Rappe, Katrin Steffanie
Ortiz-Hernandez, Monica|||0000-0001-8875-9131
Punset, Miquel|||0000-0002-1904-8667
Molmeneu, Meritxell|||0000-0002-7181-6467
Barba, Albert
Mas-Moruno, Carles|||0000-0001-8337-0872
Guillem-Marti, Jordi|||0000-0003-0307-2221
Caparrós, Cristina
Rupérez, Elisa|||0000-0001-8845-512X
Calero, José
Manzanares, María-Cristina|||0000-0002-4585-4953
Gil Mur, Javier|||0000-0002-6824-1412
Franch, Jordi|||0000-0002-3731-8552
author_role author
author2 Ortiz-Hernandez, Monica|||0000-0001-8875-9131
Punset, Miquel|||0000-0002-1904-8667
Molmeneu, Meritxell|||0000-0002-7181-6467
Barba, Albert
Mas-Moruno, Carles|||0000-0001-8337-0872
Guillem-Marti, Jordi|||0000-0003-0307-2221
Caparrós, Cristina
Rupérez, Elisa|||0000-0001-8845-512X
Calero, José
Manzanares, María-Cristina|||0000-0002-4585-4953
Gil Mur, Javier|||0000-0002-6824-1412
Franch, Jordi|||0000-0002-3731-8552
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Titanium foams
Osseointegration
Thermochemical treatment
RGD peptide
In vivo implantation
Histomorphometric evaluation
Bone on-growth
Bone in-growth
topic Titanium foams
Osseointegration
Thermochemical treatment
RGD peptide
In vivo implantation
Histomorphometric evaluation
Bone on-growth
Bone in-growth
description A lack of primary stability and osteointegration in metallic implants may result in implant loosening and failure. Adding porosity to metallic implants reduces the stress shielding effect and improves implant performance, allowing the surrounding bone tissue to grow into the scaffold. However, a bioactive surface is needed to stimulate implant osteointegration and improve mechanical stability. In this study, porous titanium implants were produced via powder sintering to create different porous diameters and open interconnectivity. Two strategies were used to generate a bioactive surface on the metallic foams: (1) an inorganic alkali thermochemical treatment, (2) grafting a cell adhesive tripeptide (RGD). RGD peptides exhibit an affinity for integrins expressed by osteoblasts, and have been reported to improve osteoblast adhesion, whereas the thermochemical treatment is known to improve titanium implant osseointegration upon implantation. Bioactivated scaffolds and control samples were implanted into the tibiae of rabbits to analyze the effect of these two strategies in vivo regarding bone tissue regeneration through interconnected porosity. Histomorphometric evaluation was performed at 4 and 12 weeks after implantation. Bone-to-implant contact (BIC) and bone in-growth and on-growth were evaluated in different regions of interest (ROIs) inside and outside the implant. The results of this study show that after a long-term postoperative period, the RGD-coated samples presented higher quantification values of quantified newly formed bone tissue in the implant's outer area. However, the total analyzed bone in-growth was observed to be slightly greater in the scaffolds treated with alkali thermochemical treatment. These results suggest that both strategies contribute to enhancing porous metallic implant stability and osteointegration, and a combination of both strategies might be worth pursuing.
publishDate 2022
dc.date.none.fl_str_mv 2
2022-01-01
2022
2022-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/256059
https://dx.doi.org/urn:doi:10.3390/ijms23031750
url https://ddd.uab.cat/record/256059
https://dx.doi.org/urn:doi:10.3390/ijms23031750
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 RTI2018-098075-B-C21
Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 RTI2018-098075-BC22
Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2020-114019RB-I00
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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