Evaluation of a Loop-Mediated Isothermal Amplification Assay to Detect Carbapenemases Directly from Bronchoalveolar Lavage Fluid Spiked with Acinetobacter spp

Carbapenem-resistant Acinetobacter spp. mainly Acinetobacter baumannii are frequently causing nosocomial infections with high mortality. In this study, the efficacy of the Eazyplex® SuperBug Complete A system, based on loop-mediated isothermal amplification (LAMP), to detect the presence of carbapen...

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Autores: Moreno Morales, Javier, Vergara Gómez, Andrea, Kostyanev, Tomislav, Rodríguez Baño, Jesús, Goossens, Herman, Vila Estapé, Jordi
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/176628
Acceso en línea:https://hdl.handle.net/2445/176628
Access Level:acceso abierto
Palabra clave:Infeccions nosocomials
Enterobacteriàcies
Nosocomial infections
Enterobacteriaceae
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spelling Evaluation of a Loop-Mediated Isothermal Amplification Assay to Detect Carbapenemases Directly from Bronchoalveolar Lavage Fluid Spiked with Acinetobacter sppMoreno Morales, JavierVergara Gómez, AndreaKostyanev, TomislavRodríguez Baño, JesúsGoossens, HermanVila Estapé, JordiInfeccions nosocomialsEnterobacteriàciesNosocomial infectionsEnterobacteriaceaeCarbapenem-resistant Acinetobacter spp. mainly Acinetobacter baumannii are frequently causing nosocomial infections with high mortality. In this study, the efficacy of the Eazyplex® SuperBug Complete A system, based on loop-mediated isothermal amplification (LAMP), to detect the presence of carbapenemases in Acinetobacter spp. directly from bronchoalveolar lavage (BAL) samples was assessed. A total of 22 Acinetobacter spp. strains producing OXA-23, OXA-40, OXA-58, NDM, and IMP were selected. Eazyplex SuperBug Complete A kit, used with the Genie II device, is a molecular diagnostics kit that detects a selection of genes that express carbapenemases (bla KPC , bla NDM , bla VIM , bla OXA-48 , bla OXA-23 , bla OXA-40 , and bla OXA-58 ). Negative BAL samples were identified, McFarland solutions were prepared from each of the 22 Acinetobacter strains and serial dilutions in saline solution were made to finally spike BAL samples to a concentration of 102 and 103 CFU/ml. Fifteen concentrations out of the 44 tested out did not provide detection of the carbapenemase-producing gene, all but one being at the lowest concentration tested at 102 CFU/ml; therefore, the limit of sensitivity is 103 CFU/ml. This assay represents the kind of advantages that investing in molecular diagnostics brings to the clinical practice, allowing the identification of carbapenemases in less than 30 min with a sensitivity of 103 CFU/ml.Frontiers Media2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/176628Articles publicats en revistes (Fonaments Clínics)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.3389/fmicb.2020.597684Frontiers in Microbiology, 2021, vol. 11, num. 597684https://doi.org/10.3389/fmicb.2020.597684cc-by (c) Moreno Morales, Javier et al., 2021http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1766282026-05-27T06:46:51Z
dc.title.none.fl_str_mv Evaluation of a Loop-Mediated Isothermal Amplification Assay to Detect Carbapenemases Directly from Bronchoalveolar Lavage Fluid Spiked with Acinetobacter spp
title Evaluation of a Loop-Mediated Isothermal Amplification Assay to Detect Carbapenemases Directly from Bronchoalveolar Lavage Fluid Spiked with Acinetobacter spp
spellingShingle Evaluation of a Loop-Mediated Isothermal Amplification Assay to Detect Carbapenemases Directly from Bronchoalveolar Lavage Fluid Spiked with Acinetobacter spp
Moreno Morales, Javier
Infeccions nosocomials
Enterobacteriàcies
Nosocomial infections
Enterobacteriaceae
title_short Evaluation of a Loop-Mediated Isothermal Amplification Assay to Detect Carbapenemases Directly from Bronchoalveolar Lavage Fluid Spiked with Acinetobacter spp
title_full Evaluation of a Loop-Mediated Isothermal Amplification Assay to Detect Carbapenemases Directly from Bronchoalveolar Lavage Fluid Spiked with Acinetobacter spp
title_fullStr Evaluation of a Loop-Mediated Isothermal Amplification Assay to Detect Carbapenemases Directly from Bronchoalveolar Lavage Fluid Spiked with Acinetobacter spp
title_full_unstemmed Evaluation of a Loop-Mediated Isothermal Amplification Assay to Detect Carbapenemases Directly from Bronchoalveolar Lavage Fluid Spiked with Acinetobacter spp
title_sort Evaluation of a Loop-Mediated Isothermal Amplification Assay to Detect Carbapenemases Directly from Bronchoalveolar Lavage Fluid Spiked with Acinetobacter spp
dc.creator.none.fl_str_mv Moreno Morales, Javier
Vergara Gómez, Andrea
Kostyanev, Tomislav
Rodríguez Baño, Jesús
Goossens, Herman
Vila Estapé, Jordi
author Moreno Morales, Javier
author_facet Moreno Morales, Javier
Vergara Gómez, Andrea
Kostyanev, Tomislav
Rodríguez Baño, Jesús
Goossens, Herman
Vila Estapé, Jordi
author_role author
author2 Vergara Gómez, Andrea
Kostyanev, Tomislav
Rodríguez Baño, Jesús
Goossens, Herman
Vila Estapé, Jordi
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Infeccions nosocomials
Enterobacteriàcies
Nosocomial infections
Enterobacteriaceae
topic Infeccions nosocomials
Enterobacteriàcies
Nosocomial infections
Enterobacteriaceae
description Carbapenem-resistant Acinetobacter spp. mainly Acinetobacter baumannii are frequently causing nosocomial infections with high mortality. In this study, the efficacy of the Eazyplex® SuperBug Complete A system, based on loop-mediated isothermal amplification (LAMP), to detect the presence of carbapenemases in Acinetobacter spp. directly from bronchoalveolar lavage (BAL) samples was assessed. A total of 22 Acinetobacter spp. strains producing OXA-23, OXA-40, OXA-58, NDM, and IMP were selected. Eazyplex SuperBug Complete A kit, used with the Genie II device, is a molecular diagnostics kit that detects a selection of genes that express carbapenemases (bla KPC , bla NDM , bla VIM , bla OXA-48 , bla OXA-23 , bla OXA-40 , and bla OXA-58 ). Negative BAL samples were identified, McFarland solutions were prepared from each of the 22 Acinetobacter strains and serial dilutions in saline solution were made to finally spike BAL samples to a concentration of 102 and 103 CFU/ml. Fifteen concentrations out of the 44 tested out did not provide detection of the carbapenemase-producing gene, all but one being at the lowest concentration tested at 102 CFU/ml; therefore, the limit of sensitivity is 103 CFU/ml. This assay represents the kind of advantages that investing in molecular diagnostics brings to the clinical practice, allowing the identification of carbapenemases in less than 30 min with a sensitivity of 103 CFU/ml.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/176628
url https://hdl.handle.net/2445/176628
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3389/fmicb.2020.597684
Frontiers in Microbiology, 2021, vol. 11, num. 597684
https://doi.org/10.3389/fmicb.2020.597684
dc.rights.none.fl_str_mv cc-by (c) Moreno Morales, Javier et al., 2021
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Moreno Morales, Javier et al., 2021
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv Articles publicats en revistes (Fonaments Clínics)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.301603

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