Neuronal Cannabinoid CB1 Receptors Suppress the Growth of Melanoma Brain Metastases by Inhibiting Glutamatergic Signalling

An estimated 60% of melanoma patients develop melanoma brain metastases(MBMs). However, the molecular factors that govern the growth of MBMs are still unknown. The excitatory neurotransmitter glutamate has been shown to control the proliferation of various types of cancer cells within the brain pare...

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Detalles Bibliográficos
Autores: Costas Insúa, Carlos, Seijo Vila, Marta, Blázquez Ortiz, Cristina, Blasco Benito, Sandra, Rodríguez Baena, Francisco Javier, Marsicano, Giovanni, Pérez Gómez, Eduardo, Sánchez García, María Cristina, Sánchez Laorden, Berta, Guzmán Pastor, Manuel
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/95971
Acceso en línea:https://hdl.handle.net/20.500.14352/95971
Access Level:acceso abierto
Palabra clave:616-006.04
Cannabinoid receptor
Endocannabinoid system
Melanoma
Brain metastasis
Glutamate
NMDA receptor
Cell proliferation
Oncología
Ciencias Biomédicas
3207 Patología
3207.13 Oncología
Descripción
Sumario:An estimated 60% of melanoma patients develop melanoma brain metastases(MBMs). However, the molecular factors that govern the growth of MBMs are still unknown. The excitatory neurotransmitter glutamate has been shown to control the proliferation of various types of cancer cells within the brain parenchyma, but the cellular sources and molecular mechanisms involved in this process remain unclear. By their well-known role in inhibiting synaptic glutamate release, cannabinoid CB1 receptors (CB1Rs) located on glutamatergic nerve terminals are conceivably well-positioned to control the growth of MBMs. In silico data mining in cancer-genome atlases and in vitro studies with melanoma cell lines supported that a glutamate-NMDA receptor axis drives melanoma cell proliferation. Strikingly, grafting melanoma cells into the brain of mice lacking CB1Rs selectively in glutamatergic neurons increased tumour size and concomitantly activated NMDA receptors on tumour cells. Altogether, our findings reveal an unprecedented role of neuronal CB1Rs in controlling MBMs.