Rapid and simple dual extraction for the analysis of lipids and autoantigenic peptides within phosphatidylserine-liposomes

Autoimmune diseases are a major health concern in developed countries. Currently, only palliative treatments based on anti-inflammatories and immunosuppressors are available. A novel antigen-specific therapy that uses a physiological process of tolerance generation is being developed. This is plausi...

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Autores: El Ouahabi, Oumaima, Mancera Arteu, Montserrat, Latorre, Irene, Salvadó, Míriam, Rodríguez-Vidal, Sílvia, Sanz Nebot, María Victoria
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:dnet:recercat____::8090b6039888573bdb4e289fd75dfe6e
Acceso en línea:https://hdl.handle.net/2445/229682
Access Level:acceso abierto
Palabra clave:Liposomes
Cromatografia de líquids
Pèptids
Liquid chromatography
Peptides
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spelling Rapid and simple dual extraction for the analysis of lipids and autoantigenic peptides within phosphatidylserine-liposomesEl Ouahabi, OumaimaMancera Arteu, MontserratLatorre, IreneSalvadó, MíriamRodríguez-Vidal, SílviaSanz Nebot, María VictoriaLiposomesCromatografia de líquidsPèptidsLiposomesLiquid chromatographyPeptidesAutoimmune diseases are a major health concern in developed countries. Currently, only palliative treatments based on anti-inflammatories and immunosuppressors are available. A novel antigen-specific therapy that uses a physiological process of tolerance generation is being developed. This is plausible by using phosphatidylserine rich liposomes (PS-liposomes), which bio-mimic apoptotic cells, encapsulated with the autoantigen responsible of generating the autoimmunity. In this way, tolerance against the own cells or tissues that were considered hostile can be achieved. In addition, only by changing the encapsulated peptide, different autoimmune diseases can be treated. Efficacy of this approach was demonstrated in type I diabetes, rheumatoid arthritis, multiple sclerosis, and myasthenia gravis. In the regulatory pre-clinical phase, analytical methodologies to evaluate the quality of the product need to be developed. In this regard, identification and quantification of the encapsulated peptide and lipids are considered critical quality attributes. In this study, a rapid and simple liquid–liquid extraction procedure, based on Bligh-Dyer method, is described for dual extraction of peptides and lipids within PS-liposomes formulation. This single step allows the separation of lipids and the encapsulated peptide in two different phases. For the subsequent analysis, two different HPLC methods were developed. The organic phase, which contains the lipids was analysed by HPLC-ELSD, while the aqueous phase, containing the encapsulated peptide, was analysed by HPLC-UV. Both methods were also validated in terms of accuracy, precision, linearity, LOD and LOQ. The extraction procedure has demonstrated highly efficient separation of lipids and peptides, avoiding interferences between them in the quantification.Elsevier B.V.2026202620242026info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion7 p.application/pdfhttps://hdl.handle.net/2445/229682Articles publicats en revistes (Enginyeria Química i Química Analítica)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1016/j.microc.2024.111420Microchemical Journal, 2024, vol. 206, p. 111420https://doi.org/10.1016/j.microc.2024.111420cc-by-nc-nd (c) El Ouahabi, Oumaima et al., 2024http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:dnet:recercat____::8090b6039888573bdb4e289fd75dfe6e2026-05-29T05:05:01Z
dc.title.none.fl_str_mv Rapid and simple dual extraction for the analysis of lipids and autoantigenic peptides within phosphatidylserine-liposomes
title Rapid and simple dual extraction for the analysis of lipids and autoantigenic peptides within phosphatidylserine-liposomes
spellingShingle Rapid and simple dual extraction for the analysis of lipids and autoantigenic peptides within phosphatidylserine-liposomes
El Ouahabi, Oumaima
Liposomes
Cromatografia de líquids
Pèptids
Liposomes
Liquid chromatography
Peptides
title_short Rapid and simple dual extraction for the analysis of lipids and autoantigenic peptides within phosphatidylserine-liposomes
title_full Rapid and simple dual extraction for the analysis of lipids and autoantigenic peptides within phosphatidylserine-liposomes
title_fullStr Rapid and simple dual extraction for the analysis of lipids and autoantigenic peptides within phosphatidylserine-liposomes
title_full_unstemmed Rapid and simple dual extraction for the analysis of lipids and autoantigenic peptides within phosphatidylserine-liposomes
title_sort Rapid and simple dual extraction for the analysis of lipids and autoantigenic peptides within phosphatidylserine-liposomes
dc.creator.none.fl_str_mv El Ouahabi, Oumaima
Mancera Arteu, Montserrat
Latorre, Irene
Salvadó, Míriam
Rodríguez-Vidal, Sílvia
Sanz Nebot, María Victoria
author El Ouahabi, Oumaima
author_facet El Ouahabi, Oumaima
Mancera Arteu, Montserrat
Latorre, Irene
Salvadó, Míriam
Rodríguez-Vidal, Sílvia
Sanz Nebot, María Victoria
author_role author
author2 Mancera Arteu, Montserrat
Latorre, Irene
Salvadó, Míriam
Rodríguez-Vidal, Sílvia
Sanz Nebot, María Victoria
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Liposomes
Cromatografia de líquids
Pèptids
Liposomes
Liquid chromatography
Peptides
topic Liposomes
Cromatografia de líquids
Pèptids
Liposomes
Liquid chromatography
Peptides
description Autoimmune diseases are a major health concern in developed countries. Currently, only palliative treatments based on anti-inflammatories and immunosuppressors are available. A novel antigen-specific therapy that uses a physiological process of tolerance generation is being developed. This is plausible by using phosphatidylserine rich liposomes (PS-liposomes), which bio-mimic apoptotic cells, encapsulated with the autoantigen responsible of generating the autoimmunity. In this way, tolerance against the own cells or tissues that were considered hostile can be achieved. In addition, only by changing the encapsulated peptide, different autoimmune diseases can be treated. Efficacy of this approach was demonstrated in type I diabetes, rheumatoid arthritis, multiple sclerosis, and myasthenia gravis. In the regulatory pre-clinical phase, analytical methodologies to evaluate the quality of the product need to be developed. In this regard, identification and quantification of the encapsulated peptide and lipids are considered critical quality attributes. In this study, a rapid and simple liquid–liquid extraction procedure, based on Bligh-Dyer method, is described for dual extraction of peptides and lipids within PS-liposomes formulation. This single step allows the separation of lipids and the encapsulated peptide in two different phases. For the subsequent analysis, two different HPLC methods were developed. The organic phase, which contains the lipids was analysed by HPLC-ELSD, while the aqueous phase, containing the encapsulated peptide, was analysed by HPLC-UV. Both methods were also validated in terms of accuracy, precision, linearity, LOD and LOQ. The extraction procedure has demonstrated highly efficient separation of lipids and peptides, avoiding interferences between them in the quantification.
publishDate 2024
dc.date.none.fl_str_mv 2024
2026
2026
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/229682
url https://hdl.handle.net/2445/229682
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1016/j.microc.2024.111420
Microchemical Journal, 2024, vol. 206, p. 111420
https://doi.org/10.1016/j.microc.2024.111420
dc.rights.none.fl_str_mv cc-by-nc-nd (c) El Ouahabi, Oumaima et al., 2024
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc-nd (c) El Ouahabi, Oumaima et al., 2024
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 7 p.
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Articles publicats en revistes (Enginyeria Química i Química Analítica)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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