Endobacter medicaginis gen. nov., sp. nov., isolated from alfalfa nodules in an acidic soil

18 Páginas, 4 figuras.-- The definitive versión is avaible at http://www.sgm.ac.uk/

Detalles Bibliográficos
Autores: Ramírez Bahena, M. Helena, Tejedor, Carmen, Martín, Isidro, Velázquez Pérez, Encarna, Peix, Álvaro
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2012
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/96972
Acceso en línea:http://hdl.handle.net/10261/96972
Access Level:acceso abierto
Palabra clave:Endophytes
Medicago
Nodules
Acetobacteraceae
Endobacter
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spelling Endobacter medicaginis gen. nov., sp. nov., isolated from alfalfa nodules in an acidic soilRamírez Bahena, M. HelenaTejedor, CarmenMartín, IsidroVelázquez Pérez, EncarnaPeix, ÁlvaroEndophytesMedicagoNodulesAcetobacteraceaeEndobacter18 Páginas, 4 figuras.-- The definitive versión is avaible at http://www.sgm.ac.uk/A bacterial strain designated M1MS02T was isolated from a surface-sterilized nodule of Medicago sativa in Zamora (Spain). The 16S rRNA gene sequence of this strain showed 96.5 and 96.2 % similarity, respectively, with respect to Gluconacetobacter liquefaciens IFO 12388T and Granulibacter bethesdensis CGDNIH1T from the family Acetobacteraceae. The novel isolate was a Gram-stain-negative, non-sporulating, aerobic coccoid to rod-shaped bacterium that was motile by a subpolar flagellum. The major fatty acid was C18 : 1ω7c and the major ubiquinone was Q-10. The lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, two aminophospholipids, three aminolipids, four glycolipids, two phospholipids and one lipid. Strain M1MS02T was catalase-positive and oxidase- and urease-negative. Acetate and lactate were not oxidized. Acetic acid was produced from ethanol in culture media supplemented with 2 % CaCO3. Ammonium sulphate was assimilated in glucose medium. The strain produced dihydroxyacetone from glycerol. Phylogenetic and phenotypic analyses commonly used to differentiate genera within the family Acetobacteraceae showed that strain M1MS02T should be classified as representing a novel species of a new genus within this family, for which the name Endobacter medicaginis gen. nov., sp. nov. is proposed. The type strain of the type species is M1MS02T ( = LMG 26838T = CECT 8088T). To our knowledge, this is the first report of a member of the Acetobacteraceae occurring as a legume nodule endophyte.This research was funded by Junta de Castilla y León (Regional Government, Spain) 215 under research project CSI02A09 and by MICINN (Central Government, Spain) under 216 research project AGL2010-17380. MHRB is recipient of a JAE-Doc researcher contract 217 from CSIC. We thank Dr. J. Euzeby for his valuable help in providing the correct 218 etymology for the name of the new taxon, and M. Ortiz for technical assistance in 219 electron microscopy.Peer reviewedSociety for General Microbiology2012info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/96972reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/doi:10.1099/ijs.0.041368-0info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/969722026-05-22T06:33:51Z
dc.title.none.fl_str_mv Endobacter medicaginis gen. nov., sp. nov., isolated from alfalfa nodules in an acidic soil
title Endobacter medicaginis gen. nov., sp. nov., isolated from alfalfa nodules in an acidic soil
spellingShingle Endobacter medicaginis gen. nov., sp. nov., isolated from alfalfa nodules in an acidic soil
Ramírez Bahena, M. Helena
Endophytes
Medicago
Nodules
Acetobacteraceae
Endobacter
title_short Endobacter medicaginis gen. nov., sp. nov., isolated from alfalfa nodules in an acidic soil
title_full Endobacter medicaginis gen. nov., sp. nov., isolated from alfalfa nodules in an acidic soil
title_fullStr Endobacter medicaginis gen. nov., sp. nov., isolated from alfalfa nodules in an acidic soil
title_full_unstemmed Endobacter medicaginis gen. nov., sp. nov., isolated from alfalfa nodules in an acidic soil
title_sort Endobacter medicaginis gen. nov., sp. nov., isolated from alfalfa nodules in an acidic soil
dc.creator.none.fl_str_mv Ramírez Bahena, M. Helena
Tejedor, Carmen
Martín, Isidro
Velázquez Pérez, Encarna
Peix, Álvaro
author Ramírez Bahena, M. Helena
author_facet Ramírez Bahena, M. Helena
Tejedor, Carmen
Martín, Isidro
Velázquez Pérez, Encarna
Peix, Álvaro
author_role author
author2 Tejedor, Carmen
Martín, Isidro
Velázquez Pérez, Encarna
Peix, Álvaro
author2_role author
author
author
author
dc.subject.none.fl_str_mv Endophytes
Medicago
Nodules
Acetobacteraceae
Endobacter
topic Endophytes
Medicago
Nodules
Acetobacteraceae
Endobacter
description 18 Páginas, 4 figuras.-- The definitive versión is avaible at http://www.sgm.ac.uk/
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/96972
url http://hdl.handle.net/10261/96972
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/doi:10.1099/ijs.0.041368-0
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Society for General Microbiology
publisher.none.fl_str_mv Society for General Microbiology
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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