Molecular effects of polystyrene nanoplastics toxicity in zebrafish embryos (Danio rerio)

Plastics pose a health hazard to living beings and the environment. Plastic degradation produces nano-sized plastic particles (NPs) that end up in terrestrial and aquatic ecosystems, including oceans, rivers, and lakes. Their presence in air, drinking water, sediments, food, and personal care produc...

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Authors: Martin-Folgar, Raquel, Torres-Ruiz, Mónica, De Alba-González, Mercedes, Cañas Portilla, Ana Isabel, González-Caballero, MCarmen, Morales, Mónica
Format: article
Publication Date:2023
Country:España
Institution:Instituto de Salud Carlos III (ISCIII)
Repository:Repisalud
Language:English
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/26091
Online Access:https://hdl.handle.net/20.500.12105/26091
Access Level:Open access
Keyword:Animals
Ecosystem
Microplastics
Plastics
Polystyrenes
Superoxide Dismutase
Water Pollutants, Chemical
Zebrafish
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spelling Molecular effects of polystyrene nanoplastics toxicity in zebrafish embryos (Danio rerio)Martin-Folgar, RaquelTorres-Ruiz, MónicaDe Alba-González, MercedesCañas Portilla, Ana IsabelGonzález-Caballero, MCarmenMorales, MónicaAnimalsEcosystemMicroplasticsPlasticsPolystyrenesPolystyrenesSuperoxide DismutaseWater Pollutants, ChemicalZebrafishPlastics pose a health hazard to living beings and the environment. Plastic degradation produces nano-sized plastic particles (NPs) that end up in terrestrial and aquatic ecosystems, including oceans, rivers, and lakes. Their presence in air, drinking water, sediments, food, and personal care products leads to a variety of exposure routes for living beings, including humans. The toxicity mechanisms of these nanomaterials (NMs) in living organisms and ecosystems are currently unknown, making it a priority to understand their effects at the molecular and cellular levels. The zebrafish (Zf) (Danio rerio) is a model organism which has a high homology with humans and has been widely used to assess the hazard of different xenobiotics. In this study, the expression changes of different genes in 120 hpf Zf embryos (Zfe) after exposure to polystyrene (PS) NPs (30 nm) at concentrations of 0.1, 0.5 and 3 ppm were investigated. The results showed that the gene encoding heat shock protein (hsp70) was down-regulated in a dose-dependent manner. The genes encoding superoxide dismutase (SOD 1 and SOD 2), apoptotic genes (cas 1 and cas 8) and interleukin 1-β (il1β) were activated at the concentration of 3 ppm PS NP, while the anti-apoptotic gene Bcl2α was inhibited at 0.5 and 3 ppm. In addition, the neurotransmitter-related gene Acetyl-Cholinesterase (ache) was significantly inhibited and the DNA repair genes (gadd45α and rad51) were also down-regulated. In contrast, the mitochondrial metabolism-related gene cox1 did not alter its expression in any of the treatments. Most of the changes in gene expression occurred at the highest concentration of NPs. Overall, the results indicated that NPs generated cellular stress that caused certain alterations in normal gene expression (oxidative stress, apoptotic and inflammatory processes, neurotoxicity and anti-apoptotic proteins), but did not cause any mortality after 120 hpf exposure at the three concentrations assayed. These results highlight the need for further studies investigating the effects, at the molecular level, of these materials in humans and other living organisms.ElsevierInstituto de Salud Carlos IIINational University of Distance Education (España)20252025-01-2220232023-01-0120232023-01-01research articlehttp://purl.org/coar/resource_type/c_2df8fbb1SMURhttp://purl.org/coar/version/c_71e4c1898caa6e32info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.12105/26091reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/260912026-06-12T12:43:37Z
dc.title.none.fl_str_mv Molecular effects of polystyrene nanoplastics toxicity in zebrafish embryos (Danio rerio)
title Molecular effects of polystyrene nanoplastics toxicity in zebrafish embryos (Danio rerio)
spellingShingle Molecular effects of polystyrene nanoplastics toxicity in zebrafish embryos (Danio rerio)
Martin-Folgar, Raquel
Animals
Ecosystem
Microplastics
Plastics
Polystyrenes
Polystyrenes
Superoxide Dismutase
Water Pollutants, Chemical
Zebrafish
title_short Molecular effects of polystyrene nanoplastics toxicity in zebrafish embryos (Danio rerio)
title_full Molecular effects of polystyrene nanoplastics toxicity in zebrafish embryos (Danio rerio)
title_fullStr Molecular effects of polystyrene nanoplastics toxicity in zebrafish embryos (Danio rerio)
title_full_unstemmed Molecular effects of polystyrene nanoplastics toxicity in zebrafish embryos (Danio rerio)
title_sort Molecular effects of polystyrene nanoplastics toxicity in zebrafish embryos (Danio rerio)
dc.creator.none.fl_str_mv Martin-Folgar, Raquel
Torres-Ruiz, Mónica
De Alba-González, Mercedes
Cañas Portilla, Ana Isabel
González-Caballero, MCarmen
Morales, Mónica
author Martin-Folgar, Raquel
author_facet Martin-Folgar, Raquel
Torres-Ruiz, Mónica
De Alba-González, Mercedes
Cañas Portilla, Ana Isabel
González-Caballero, MCarmen
Morales, Mónica
author_role author
author2 Torres-Ruiz, Mónica
De Alba-González, Mercedes
Cañas Portilla, Ana Isabel
González-Caballero, MCarmen
Morales, Mónica
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Salud Carlos III
National University of Distance Education (España)
dc.subject.none.fl_str_mv Animals
Ecosystem
Microplastics
Plastics
Polystyrenes
Polystyrenes
Superoxide Dismutase
Water Pollutants, Chemical
Zebrafish
topic Animals
Ecosystem
Microplastics
Plastics
Polystyrenes
Polystyrenes
Superoxide Dismutase
Water Pollutants, Chemical
Zebrafish
description Plastics pose a health hazard to living beings and the environment. Plastic degradation produces nano-sized plastic particles (NPs) that end up in terrestrial and aquatic ecosystems, including oceans, rivers, and lakes. Their presence in air, drinking water, sediments, food, and personal care products leads to a variety of exposure routes for living beings, including humans. The toxicity mechanisms of these nanomaterials (NMs) in living organisms and ecosystems are currently unknown, making it a priority to understand their effects at the molecular and cellular levels. The zebrafish (Zf) (Danio rerio) is a model organism which has a high homology with humans and has been widely used to assess the hazard of different xenobiotics. In this study, the expression changes of different genes in 120 hpf Zf embryos (Zfe) after exposure to polystyrene (PS) NPs (30 nm) at concentrations of 0.1, 0.5 and 3 ppm were investigated. The results showed that the gene encoding heat shock protein (hsp70) was down-regulated in a dose-dependent manner. The genes encoding superoxide dismutase (SOD 1 and SOD 2), apoptotic genes (cas 1 and cas 8) and interleukin 1-β (il1β) were activated at the concentration of 3 ppm PS NP, while the anti-apoptotic gene Bcl2α was inhibited at 0.5 and 3 ppm. In addition, the neurotransmitter-related gene Acetyl-Cholinesterase (ache) was significantly inhibited and the DNA repair genes (gadd45α and rad51) were also down-regulated. In contrast, the mitochondrial metabolism-related gene cox1 did not alter its expression in any of the treatments. Most of the changes in gene expression occurred at the highest concentration of NPs. Overall, the results indicated that NPs generated cellular stress that caused certain alterations in normal gene expression (oxidative stress, apoptotic and inflammatory processes, neurotoxicity and anti-apoptotic proteins), but did not cause any mortality after 120 hpf exposure at the three concentrations assayed. These results highlight the need for further studies investigating the effects, at the molecular level, of these materials in humans and other living organisms.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-01-01
2023
2023-01-01
2025
2025-01-22
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
SMUR
http://purl.org/coar/version/c_71e4c1898caa6e32
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.12105/26091
url https://hdl.handle.net/20.500.12105/26091
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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