3D Printed porous polyamide macrocapsule combined with alginate microcapsules for safer cell-based therapies
Cell microencapsulation is an attractive strategy for cell-based therapies that allows the implantation of genetically engineered cells and the continuous delivery of de novo produced therapeutic products. However, the establishment of a way to retrieve the implanted encapsulated cells in case the t...
| Autores: | , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2018 |
| País: | España |
| Institución: | Universitat Politècnica de Catalunya (UPC) |
| Repositorio: | UPCommons. Portal del coneixement obert de la UPC |
| Idioma: | inglés |
| OAI Identifier: | oai:upcommons.upc.edu:2117/342145 |
| Acceso en línea: | https://hdl.handle.net/2117/342145 https://dx.doi.org/10.1038/s41598-018-26869-5 |
| Access Level: | acceso abierto |
| Palabra clave: | Tissue engineering Three-dimensional printing Biomedical materials Enginyeria de teixits Impressió 3D Materials biomèdics Àrees temàtiques de la UPC::Enginyeria dels materials |
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3D Printed porous polyamide macrocapsule combined with alginate microcapsules for safer cell-based therapiesSaenz del Burgo, LauraCiriza, JesúsEspona Noguera, Albert|||0000-0002-3681-030XIlla, XaviCabruja, EnricOrive, GorkaHernández, Rosa MariaVilla, RosaPedraz, Jose LuizÁlvarez, MarTissue engineeringThree-dimensional printingBiomedical materialsEnginyeria de teixitsImpressió 3DMaterials biomèdicsÀrees temàtiques de la UPC::Enginyeria dels materialsCell microencapsulation is an attractive strategy for cell-based therapies that allows the implantation of genetically engineered cells and the continuous delivery of de novo produced therapeutic products. However, the establishment of a way to retrieve the implanted encapsulated cells in case the treatment needs to be halted or when cells need to be renewed is still a big challenge. The combination of micro and macroencapsulation approaches could provide the requirements to achieve a proper immunoisolation, while maintaining the cells localized into the body. We present the development and characterization of a porous implantable macrocapsule device for the loading of microencapsulated cells. The device was fabricated in polyamide by selective laser sintering (SLS), with controlled porosity defined by the design and the sintering conditions. Two types of microencapsulated cells were tested in order to evaluate the suitability of this device; erythropoietin (EPO) producing C2C12 myoblasts and Vascular Endothelial Growth Factor (VEGF) producing BHK fibroblasts. Results showed that, even if the metabolic activity of these cells decreased over time, the levels of therapeutic protein that were produced and, importantly, released to the media were stable.Peer ReviewedNature20182018-12-0120212021-03-22journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/2117/342145https://dx.doi.org/10.1038/s41598-018-26869-5reponame:UPCommons. Portal del coneixement obert de la UPCinstname:Universitat Politècnica de Catalunya (UPC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivs 3.0 Spainhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:upcommons.upc.edu:2117/3421452026-05-27T15:37:01Z |
| dc.title.none.fl_str_mv |
3D Printed porous polyamide macrocapsule combined with alginate microcapsules for safer cell-based therapies |
| title |
3D Printed porous polyamide macrocapsule combined with alginate microcapsules for safer cell-based therapies |
| spellingShingle |
3D Printed porous polyamide macrocapsule combined with alginate microcapsules for safer cell-based therapies Saenz del Burgo, Laura Tissue engineering Three-dimensional printing Biomedical materials Enginyeria de teixits Impressió 3D Materials biomèdics Àrees temàtiques de la UPC::Enginyeria dels materials |
| title_short |
3D Printed porous polyamide macrocapsule combined with alginate microcapsules for safer cell-based therapies |
| title_full |
3D Printed porous polyamide macrocapsule combined with alginate microcapsules for safer cell-based therapies |
| title_fullStr |
3D Printed porous polyamide macrocapsule combined with alginate microcapsules for safer cell-based therapies |
| title_full_unstemmed |
3D Printed porous polyamide macrocapsule combined with alginate microcapsules for safer cell-based therapies |
| title_sort |
3D Printed porous polyamide macrocapsule combined with alginate microcapsules for safer cell-based therapies |
| dc.creator.none.fl_str_mv |
Saenz del Burgo, Laura Ciriza, Jesús Espona Noguera, Albert|||0000-0002-3681-030X Illa, Xavi Cabruja, Enric Orive, Gorka Hernández, Rosa Maria Villa, Rosa Pedraz, Jose Luiz Álvarez, Mar |
| author |
Saenz del Burgo, Laura |
| author_facet |
Saenz del Burgo, Laura Ciriza, Jesús Espona Noguera, Albert|||0000-0002-3681-030X Illa, Xavi Cabruja, Enric Orive, Gorka Hernández, Rosa Maria Villa, Rosa Pedraz, Jose Luiz Álvarez, Mar |
| author_role |
author |
| author2 |
Ciriza, Jesús Espona Noguera, Albert|||0000-0002-3681-030X Illa, Xavi Cabruja, Enric Orive, Gorka Hernández, Rosa Maria Villa, Rosa Pedraz, Jose Luiz Álvarez, Mar |
| author2_role |
author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Tissue engineering Three-dimensional printing Biomedical materials Enginyeria de teixits Impressió 3D Materials biomèdics Àrees temàtiques de la UPC::Enginyeria dels materials |
| topic |
Tissue engineering Three-dimensional printing Biomedical materials Enginyeria de teixits Impressió 3D Materials biomèdics Àrees temàtiques de la UPC::Enginyeria dels materials |
| description |
Cell microencapsulation is an attractive strategy for cell-based therapies that allows the implantation of genetically engineered cells and the continuous delivery of de novo produced therapeutic products. However, the establishment of a way to retrieve the implanted encapsulated cells in case the treatment needs to be halted or when cells need to be renewed is still a big challenge. The combination of micro and macroencapsulation approaches could provide the requirements to achieve a proper immunoisolation, while maintaining the cells localized into the body. We present the development and characterization of a porous implantable macrocapsule device for the loading of microencapsulated cells. The device was fabricated in polyamide by selective laser sintering (SLS), with controlled porosity defined by the design and the sintering conditions. Two types of microencapsulated cells were tested in order to evaluate the suitability of this device; erythropoietin (EPO) producing C2C12 myoblasts and Vascular Endothelial Growth Factor (VEGF) producing BHK fibroblasts. Results showed that, even if the metabolic activity of these cells decreased over time, the levels of therapeutic protein that were produced and, importantly, released to the media were stable. |
| publishDate |
2018 |
| dc.date.none.fl_str_mv |
2018 2018-12-01 2021 2021-03-22 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/2117/342145 https://dx.doi.org/10.1038/s41598-018-26869-5 |
| url |
https://hdl.handle.net/2117/342145 https://dx.doi.org/10.1038/s41598-018-26869-5 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Attribution-NonCommercial-NoDerivs 3.0 Spain http://creativecommons.org/licenses/by-nc-nd/3.0/es/ |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Attribution-NonCommercial-NoDerivs 3.0 Spain http://creativecommons.org/licenses/by-nc-nd/3.0/es/ |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Nature |
| publisher.none.fl_str_mv |
Nature |
| dc.source.none.fl_str_mv |
reponame:UPCommons. Portal del coneixement obert de la UPC instname:Universitat Politècnica de Catalunya (UPC) |
| instname_str |
Universitat Politècnica de Catalunya (UPC) |
| reponame_str |
UPCommons. Portal del coneixement obert de la UPC |
| collection |
UPCommons. Portal del coneixement obert de la UPC |
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| repository.mail.fl_str_mv |
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1869408657305763840 |
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15,300719 |