Raw data: Synthetically-induced Arabidopsis thaliana autotetraploids provide insights in the analysis of meiotic mutants with altered crossover frequency
• Mutations affecting crossover frequency and distribution lead to the presence of univalents during meiosis, giving rise to aneuploid gametes and sterility. These mutations may have a different effect after chromosome doubling. The combination of altered ploidy and mutations could be potentially us...
| Autores: | , , , , , , |
|---|---|
| Formato: | conjunto de datos |
| Fecha de publicación: | 2023 |
| País: | España |
| Recursos: | Universidad Complutense de Madrid (UCM) |
| Repositorio: | Docta Complutense |
| Idioma: | inglés |
| OAI Identifier: | oai:docta.ucm.es:20.500.14352/88106 |
| Acesso em linha: | https://hdl.handle.net/20.500.14352/88106 |
| Access Level: | acceso abierto |
| Palavra-chave: | 575 576.35 Arabidopsis Autopolyploid Chiasma Colchicine Crossover Homologous recombination Meiosis Polyploid Genética Biología celular (Biología) 2407 Biología Celular 2409 Genética |
| Resumo: | • Mutations affecting crossover frequency and distribution lead to the presence of univalents during meiosis, giving rise to aneuploid gametes and sterility. These mutations may have a different effect after chromosome doubling. The combination of altered ploidy and mutations could be potentially useful to gain new insights into the mechanisms and regulation of meiotic recombination, however, studies using autopolyploid meiotic mutants are scarce. • Here, we have analyzed the cytogenetic consequences in colchicine-induced autotetraploids (colchiploids) from different Arabidopsis mutants with an altered crossover frequency. • We have found that there are three types of mutants: i) mutants in which chiasma frequency is doubled after chromosome duplication (zip4, mus81), as in the control; ii) mutants in which polyploidy leads to higher-than expected increase in chiasma frequency (asy1, mer3, hei10, mlh3); and iii) mutants in which the rise in chiasma frequency produced by the presence of two extra chromosomal sets is less than doubled (msh5, fancm). In addition, the proportion of class I/class II crossovers varies after chromosome duplication in the control. • The results obtained reveal the potential of colchiploid meiotic mutants for better understanding of the function of key proteins during plant meiosis. This is especially relevant considering that most crops are polyploids. |
|---|