Diversity in oat potential immunogenicity: basis for the selection of oat varieties with no toxicity in coeliac disease

Background and aims Coeliac disease (CD) is triggered by an abnormal reaction to gluten. Peptides resulting from partially digested gluten of wheat, barley or rye cause inflammation of the small intestinal mucosa. Previous contradictory studies suggest that oats may trigger the abnormal immunologica...

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Autores: Comino Montilla, Isabel María, Lorenzo Barrios, Laura de, Cornell, Hugh, López Casado, Miguel Ángel, Barro, Francisco, Sousa Martín, Carolina
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2011
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/58805
Acceso en línea:http://hdl.handle.net/11441/58805
https://doi.org/10.1136/gut.2010.225268
Access Level:acceso abierto
Palabra clave:Coeliac disease
oats
moAb
T cells
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spelling Diversity in oat potential immunogenicity: basis for the selection of oat varieties with no toxicity in coeliac diseaseComino Montilla, Isabel MaríaLorenzo Barrios, Laura deCornell, HughLópez Casado, Miguel ÁngelBarro, FranciscoSousa Martín, CarolinaCoeliac diseaseoatsmoAbT cellsBackground and aims Coeliac disease (CD) is triggered by an abnormal reaction to gluten. Peptides resulting from partially digested gluten of wheat, barley or rye cause inflammation of the small intestinal mucosa. Previous contradictory studies suggest that oats may trigger the abnormal immunological response in patients with CD. Monoclonal antibodies (moAbs) against the main immunotoxic 33-mer peptide (A1 and G12) react strongly against wheat, barley and rye but have less reactivity against oats. The stated aim of this study is to test whether this observed reactivity could be related to the potential toxicity of oats for patients with CD. Methods In the present study, different oat varieties, controlled for their purity and by their distinct protein pattern, were used to examine differences in moAb G12 recognition by ELISA and western blot. Immunogenicity of oat varieties was determined by 33-mer concentration, T cell proliferation and interferon γ production. Results Three groups of oat cultivars reacting differently against moAb G12 could be distinguished: a group with considerable affinity, a group showing slight reactivity and a third with no detectable reactivity. The immunogenicity of the three types of oats as well as that of a positive and negative control was determined with isolated peripheral blood mononuclear T cells from patients with CD by measurement of cell proliferation and interferon γ release. A direct correlation of the reactivity with G12 and the immunogenicity of the different prolamins was observed. Conclusions The results showed that the reactivity of the moAb G12 is proportional to the potential immunotoxicity of the cereal cultivar. These differences may explain the different clinical responses observed in patients suffering from CD and open up a means to identify immunologically safe oat cultivars, which could be used to enrich a gluten-free diet.BMJ Publishing GroupMicrobiología y Parasitología2011info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/11441/58805https://doi.org/10.1136/gut.2010.225268reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésGut, 60 (7), 915-922.http://dx.doi.org/10.1136/gut.2010.225268info:eu-repo/semantics/openAccessoai:idus.us.es:11441/588052026-06-17T12:51:07Z
dc.title.none.fl_str_mv Diversity in oat potential immunogenicity: basis for the selection of oat varieties with no toxicity in coeliac disease
title Diversity in oat potential immunogenicity: basis for the selection of oat varieties with no toxicity in coeliac disease
spellingShingle Diversity in oat potential immunogenicity: basis for the selection of oat varieties with no toxicity in coeliac disease
Comino Montilla, Isabel María
Coeliac disease
oats
moAb
T cells
title_short Diversity in oat potential immunogenicity: basis for the selection of oat varieties with no toxicity in coeliac disease
title_full Diversity in oat potential immunogenicity: basis for the selection of oat varieties with no toxicity in coeliac disease
title_fullStr Diversity in oat potential immunogenicity: basis for the selection of oat varieties with no toxicity in coeliac disease
title_full_unstemmed Diversity in oat potential immunogenicity: basis for the selection of oat varieties with no toxicity in coeliac disease
title_sort Diversity in oat potential immunogenicity: basis for the selection of oat varieties with no toxicity in coeliac disease
dc.creator.none.fl_str_mv Comino Montilla, Isabel María
Lorenzo Barrios, Laura de
Cornell, Hugh
López Casado, Miguel Ángel
Barro, Francisco
Sousa Martín, Carolina
author Comino Montilla, Isabel María
author_facet Comino Montilla, Isabel María
Lorenzo Barrios, Laura de
Cornell, Hugh
López Casado, Miguel Ángel
Barro, Francisco
Sousa Martín, Carolina
author_role author
author2 Lorenzo Barrios, Laura de
Cornell, Hugh
López Casado, Miguel Ángel
Barro, Francisco
Sousa Martín, Carolina
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Microbiología y Parasitología
dc.subject.none.fl_str_mv Coeliac disease
oats
moAb
T cells
topic Coeliac disease
oats
moAb
T cells
description Background and aims Coeliac disease (CD) is triggered by an abnormal reaction to gluten. Peptides resulting from partially digested gluten of wheat, barley or rye cause inflammation of the small intestinal mucosa. Previous contradictory studies suggest that oats may trigger the abnormal immunological response in patients with CD. Monoclonal antibodies (moAbs) against the main immunotoxic 33-mer peptide (A1 and G12) react strongly against wheat, barley and rye but have less reactivity against oats. The stated aim of this study is to test whether this observed reactivity could be related to the potential toxicity of oats for patients with CD. Methods In the present study, different oat varieties, controlled for their purity and by their distinct protein pattern, were used to examine differences in moAb G12 recognition by ELISA and western blot. Immunogenicity of oat varieties was determined by 33-mer concentration, T cell proliferation and interferon γ production. Results Three groups of oat cultivars reacting differently against moAb G12 could be distinguished: a group with considerable affinity, a group showing slight reactivity and a third with no detectable reactivity. The immunogenicity of the three types of oats as well as that of a positive and negative control was determined with isolated peripheral blood mononuclear T cells from patients with CD by measurement of cell proliferation and interferon γ release. A direct correlation of the reactivity with G12 and the immunogenicity of the different prolamins was observed. Conclusions The results showed that the reactivity of the moAb G12 is proportional to the potential immunotoxicity of the cereal cultivar. These differences may explain the different clinical responses observed in patients suffering from CD and open up a means to identify immunologically safe oat cultivars, which could be used to enrich a gluten-free diet.
publishDate 2011
dc.date.none.fl_str_mv 2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11441/58805
https://doi.org/10.1136/gut.2010.225268
url http://hdl.handle.net/11441/58805
https://doi.org/10.1136/gut.2010.225268
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Gut, 60 (7), 915-922.
http://dx.doi.org/10.1136/gut.2010.225268
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv BMJ Publishing Group
publisher.none.fl_str_mv BMJ Publishing Group
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
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