Differential activity of lytic polysaccharide monooxygenases on celluloses of different crystallinity. Effectiveness in the sustainable production of cellulose nanofibrils

A series of cellulosic substrates has been produced, treated with lytic polysaccharide monooxygenase (LPMO) from Streptomyces ambofaciens (SamLPMO10C), and analyzed by high performance anion exchange chromatography (HPAEC) with pulsed amperometric detection (PAD). The activity of the bacterial LPMO...

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Autores: Valenzuela, Susana V., Valls Vidal, Cristina|||0000-0003-2307-1779, Schink, Viviane, Sanchez, Daniel, Roncero Vivero, María Blanca|||0000-0002-2694-2368, Diaz Lucea, M. Pilar, Martinez, Josefina, Javier Pastor, F.I.
Formato: artículo
Fecha de publicación:2018
País:España
Recursos:Universitat Politècnica de Catalunya (UPC)
Repositorio:UPCommons. Portal del coneixement obert de la UPC
Idioma:inglés
OAI Identifier:oai:upcommons.upc.edu:2117/125788
Acesso em linha:https://hdl.handle.net/2117/125788
https://dx.doi.org/10.1016/j.carbpol.2018.11.076
Access Level:acceso abierto
Palavra-chave:Cellulose
Nanofibers
Monooxygenases
Polysaccharide
Cellulose fibers
Crystallinity
SamLPMO10C
Monooxygenase
Nanofibrils
LPMO
NFC
Cel·lulosa -- Investigació
Nanofibres
Polisacàrids
Fibres de cel·lulosa
Àrees temàtiques de la UPC::Enginyeria paperera::Primeres matèries papereres::Cel·lulosa
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oai_identifier_str oai:upcommons.upc.edu:2117/125788
network_acronym_str ES
network_name_str España
repository_id_str
spelling Differential activity of lytic polysaccharide monooxygenases on celluloses of different crystallinity. Effectiveness in the sustainable production of cellulose nanofibrilsValenzuela, Susana V.Valls Vidal, Cristina|||0000-0003-2307-1779Schink, VivianeSanchez, DanielRoncero Vivero, María Blanca|||0000-0002-2694-2368Diaz Lucea, M. PilarMartinez, JosefinaJavier Pastor, F.I.CelluloseNanofibersMonooxygenasesPolysaccharideCellulose fibersCrystallinitySamLPMO10CMonooxygenaseCelluloseNanofibrilsLPMONFCCel·lulosa -- InvestigacióNanofibresPolisacàridsFibres de cel·lulosaÀrees temàtiques de la UPC::Enginyeria paperera::Primeres matèries papereres::Cel·lulosaA series of cellulosic substrates has been produced, treated with lytic polysaccharide monooxygenase (LPMO) from Streptomyces ambofaciens (SamLPMO10C), and analyzed by high performance anion exchange chromatography (HPAEC) with pulsed amperometric detection (PAD). The activity of the bacterial LPMO showed high variability depending on the origin and degree of crystallinity of the substrate. Additionally, we tested the effectiveness of SamLPMO10C in the nanofibrillation of flax, a high crystalline agricultural fiber, as a single pretreatment or in combination with cellulases. All pretreatments were followed by a mechanical defibrillation by high-pressure homogenization (HPH) to obtain cellulose nanofibrils (NFC). The combined LPMO-cellulase treatment showed higher fibrillation yield, optical transmittance and carboxylate content than control reactions. Therefore, it could be explored as a promising green alternative to reduce the energy consumption in the production of NFC. To our knowledge, this is the first study reporting the effect of a bacterial LPMO in nanocellulose production20192019-03-0120182018-12-13journal articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/2117/125788https://dx.doi.org/10.1016/j.carbpol.2018.11.076reponame:UPCommons. Portal del coneixement obert de la UPCinstname:Universitat Politècnica de Catalunya (UPC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivs 3.0 Spainhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:upcommons.upc.edu:2117/1257882026-05-27T15:37:01Z
dc.title.none.fl_str_mv Differential activity of lytic polysaccharide monooxygenases on celluloses of different crystallinity. Effectiveness in the sustainable production of cellulose nanofibrils
title Differential activity of lytic polysaccharide monooxygenases on celluloses of different crystallinity. Effectiveness in the sustainable production of cellulose nanofibrils
spellingShingle Differential activity of lytic polysaccharide monooxygenases on celluloses of different crystallinity. Effectiveness in the sustainable production of cellulose nanofibrils
Valenzuela, Susana V.
Cellulose
Nanofibers
Monooxygenases
Polysaccharide
Cellulose fibers
Crystallinity
SamLPMO10C
Monooxygenase
Cellulose
Nanofibrils
LPMO
NFC
Cel·lulosa -- Investigació
Nanofibres
Polisacàrids
Fibres de cel·lulosa
Àrees temàtiques de la UPC::Enginyeria paperera::Primeres matèries papereres::Cel·lulosa
title_short Differential activity of lytic polysaccharide monooxygenases on celluloses of different crystallinity. Effectiveness in the sustainable production of cellulose nanofibrils
title_full Differential activity of lytic polysaccharide monooxygenases on celluloses of different crystallinity. Effectiveness in the sustainable production of cellulose nanofibrils
title_fullStr Differential activity of lytic polysaccharide monooxygenases on celluloses of different crystallinity. Effectiveness in the sustainable production of cellulose nanofibrils
title_full_unstemmed Differential activity of lytic polysaccharide monooxygenases on celluloses of different crystallinity. Effectiveness in the sustainable production of cellulose nanofibrils
title_sort Differential activity of lytic polysaccharide monooxygenases on celluloses of different crystallinity. Effectiveness in the sustainable production of cellulose nanofibrils
dc.creator.none.fl_str_mv Valenzuela, Susana V.
Valls Vidal, Cristina|||0000-0003-2307-1779
Schink, Viviane
Sanchez, Daniel
Roncero Vivero, María Blanca|||0000-0002-2694-2368
Diaz Lucea, M. Pilar
Martinez, Josefina
Javier Pastor, F.I.
author Valenzuela, Susana V.
author_facet Valenzuela, Susana V.
Valls Vidal, Cristina|||0000-0003-2307-1779
Schink, Viviane
Sanchez, Daniel
Roncero Vivero, María Blanca|||0000-0002-2694-2368
Diaz Lucea, M. Pilar
Martinez, Josefina
Javier Pastor, F.I.
author_role author
author2 Valls Vidal, Cristina|||0000-0003-2307-1779
Schink, Viviane
Sanchez, Daniel
Roncero Vivero, María Blanca|||0000-0002-2694-2368
Diaz Lucea, M. Pilar
Martinez, Josefina
Javier Pastor, F.I.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cellulose
Nanofibers
Monooxygenases
Polysaccharide
Cellulose fibers
Crystallinity
SamLPMO10C
Monooxygenase
Cellulose
Nanofibrils
LPMO
NFC
Cel·lulosa -- Investigació
Nanofibres
Polisacàrids
Fibres de cel·lulosa
Àrees temàtiques de la UPC::Enginyeria paperera::Primeres matèries papereres::Cel·lulosa
topic Cellulose
Nanofibers
Monooxygenases
Polysaccharide
Cellulose fibers
Crystallinity
SamLPMO10C
Monooxygenase
Cellulose
Nanofibrils
LPMO
NFC
Cel·lulosa -- Investigació
Nanofibres
Polisacàrids
Fibres de cel·lulosa
Àrees temàtiques de la UPC::Enginyeria paperera::Primeres matèries papereres::Cel·lulosa
description A series of cellulosic substrates has been produced, treated with lytic polysaccharide monooxygenase (LPMO) from Streptomyces ambofaciens (SamLPMO10C), and analyzed by high performance anion exchange chromatography (HPAEC) with pulsed amperometric detection (PAD). The activity of the bacterial LPMO showed high variability depending on the origin and degree of crystallinity of the substrate. Additionally, we tested the effectiveness of SamLPMO10C in the nanofibrillation of flax, a high crystalline agricultural fiber, as a single pretreatment or in combination with cellulases. All pretreatments were followed by a mechanical defibrillation by high-pressure homogenization (HPH) to obtain cellulose nanofibrils (NFC). The combined LPMO-cellulase treatment showed higher fibrillation yield, optical transmittance and carboxylate content than control reactions. Therefore, it could be explored as a promising green alternative to reduce the energy consumption in the production of NFC. To our knowledge, this is the first study reporting the effect of a bacterial LPMO in nanocellulose production
publishDate 2018
dc.date.none.fl_str_mv 2018
2018-12-13
2019
2019-03-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/2117/125788
https://dx.doi.org/10.1016/j.carbpol.2018.11.076
url https://hdl.handle.net/2117/125788
https://dx.doi.org/10.1016/j.carbpol.2018.11.076
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:UPCommons. Portal del coneixement obert de la UPC
instname:Universitat Politècnica de Catalunya (UPC)
instname_str Universitat Politècnica de Catalunya (UPC)
reponame_str UPCommons. Portal del coneixement obert de la UPC
collection UPCommons. Portal del coneixement obert de la UPC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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