Altered Proteomic Profile of Adipose Tissue-Derived Mesenchymal Stem Cell Exosomes from Cats with Severe Chronic Gingivostomatitis
Feline chronic gingivostomatitis (FCGS) is a pathology with a complicated therapeutic approach and with a prevalence between 0.7 and 12%. Although the etiology of the disease is diverse, feline calicivirus infection is known to be a predisposing factor. To date, the available treatment helps in cont...
| Autores: | , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2021 |
| País: | España |
| Institución: | Instituto de Salud Carlos III (ISCIII) |
| Repositorio: | Repisalud |
| Idioma: | inglés |
| OAI Identifier: | oai:repisalud.isciii.es:20.500.12105/18443 |
| Acceso en línea: | http://hdl.handle.net/20.500.12105/18443 |
| Access Level: | acceso abierto |
| Palabra clave: | Feline Feline chronic gingivostomatitis Mesenchymal stem cells Exosomes Ultra-highperformance liquid chromatography high-resolution mass spectrometry (UHPLC–HRMS) Enfermedades de los gatos Células madre mesenquimatosas Exosomas Cromatografía líquida de alta presión Espectrometría de masas Cats Humans Animals Prevalence Control Groups Down-Regulation Gene Ontology Proteomics Adipose Tissue Caliciviridae Infections Cell Communication Mass Spectrometry Immune System |
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España |
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| dc.title.none.fl_str_mv |
Altered Proteomic Profile of Adipose Tissue-Derived Mesenchymal Stem Cell Exosomes from Cats with Severe Chronic Gingivostomatitis |
| title |
Altered Proteomic Profile of Adipose Tissue-Derived Mesenchymal Stem Cell Exosomes from Cats with Severe Chronic Gingivostomatitis |
| spellingShingle |
Altered Proteomic Profile of Adipose Tissue-Derived Mesenchymal Stem Cell Exosomes from Cats with Severe Chronic Gingivostomatitis Villatoro, Antonio J. Feline Feline chronic gingivostomatitis Mesenchymal stem cells Exosomes Ultra-highperformance liquid chromatography high-resolution mass spectrometry (UHPLC–HRMS) Enfermedades de los gatos Células madre mesenquimatosas Exosomas Cromatografía líquida de alta presión Espectrometría de masas Cats Humans Animals Exosomes Prevalence Control Groups Down-Regulation Gene Ontology Proteomics Adipose Tissue Caliciviridae Infections Cell Communication Mass Spectrometry Immune System |
| title_short |
Altered Proteomic Profile of Adipose Tissue-Derived Mesenchymal Stem Cell Exosomes from Cats with Severe Chronic Gingivostomatitis |
| title_full |
Altered Proteomic Profile of Adipose Tissue-Derived Mesenchymal Stem Cell Exosomes from Cats with Severe Chronic Gingivostomatitis |
| title_fullStr |
Altered Proteomic Profile of Adipose Tissue-Derived Mesenchymal Stem Cell Exosomes from Cats with Severe Chronic Gingivostomatitis |
| title_full_unstemmed |
Altered Proteomic Profile of Adipose Tissue-Derived Mesenchymal Stem Cell Exosomes from Cats with Severe Chronic Gingivostomatitis |
| title_sort |
Altered Proteomic Profile of Adipose Tissue-Derived Mesenchymal Stem Cell Exosomes from Cats with Severe Chronic Gingivostomatitis |
| dc.creator.none.fl_str_mv |
Villatoro, Antonio J. Martín-Astorga, María del Carmen Alcoholado, Cristina Cárdenas, Casimiro Fariñas, Fernando Becerra, José Visser, Rick |
| author |
Villatoro, Antonio J. |
| author_facet |
Villatoro, Antonio J. Martín-Astorga, María del Carmen Alcoholado, Cristina Cárdenas, Casimiro Fariñas, Fernando Becerra, José Visser, Rick |
| author_role |
author |
| author2 |
Martín-Astorga, María del Carmen Alcoholado, Cristina Cárdenas, Casimiro Fariñas, Fernando Becerra, José Visser, Rick |
| author2_role |
author author author author author author |
| dc.contributor.none.fl_str_mv |
[Villatoro,AJ; Martín-Astorga,MC; Alcoholado,C; Becerra,J; Visser,R] Laboratory of Bioengineering and Tissue Regeneration, Department of Cell Biology, Genetics and Physiology, University of Málaga, IBIMA, Málaga, Spain. [Villatoro,AJ] Instituto de Inmunología Clínica y Terapia Celular (IMMUNESTEM), Málaga, Spain. [Cárdenas,C] Research Support Central Services (SCAI) of the University of Málaga, Málaga, Spain. [Fariñas,F] Grupo Ynmun, Spanish Association for the Research in Immunological and Infectious Diseases, Málaga, Spain. [Becerra,J; Visser,R] Biomedical Research Networking Center in Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN), Madrid, Spain. [Becerra,J; Visser,R] Andalusian Centre for Nanomedicine and Biotechnology-University of Málaga (BIONAND), Málaga, Spain. |
| dc.subject.none.fl_str_mv |
Feline Feline chronic gingivostomatitis Mesenchymal stem cells Exosomes Ultra-highperformance liquid chromatography high-resolution mass spectrometry (UHPLC–HRMS) Enfermedades de los gatos Células madre mesenquimatosas Exosomas Cromatografía líquida de alta presión Espectrometría de masas Cats Humans Animals Exosomes Prevalence Control Groups Down-Regulation Gene Ontology Proteomics Adipose Tissue Caliciviridae Infections Cell Communication Mass Spectrometry Immune System |
| topic |
Feline Feline chronic gingivostomatitis Mesenchymal stem cells Exosomes Ultra-highperformance liquid chromatography high-resolution mass spectrometry (UHPLC–HRMS) Enfermedades de los gatos Células madre mesenquimatosas Exosomas Cromatografía líquida de alta presión Espectrometría de masas Cats Humans Animals Exosomes Prevalence Control Groups Down-Regulation Gene Ontology Proteomics Adipose Tissue Caliciviridae Infections Cell Communication Mass Spectrometry Immune System |
| description |
Feline chronic gingivostomatitis (FCGS) is a pathology with a complicated therapeutic approach and with a prevalence between 0.7 and 12%. Although the etiology of the disease is diverse, feline calicivirus infection is known to be a predisposing factor. To date, the available treatment helps in controlling the disease, but cannot always provide a cure, which leads to a high percentage of refractory animals. Mesenchymal stem cells (MSCs) play a pivotal role in the homeostasis and reparation of different tissues and have the ability to modulate the immune system responses. This ability is, in part, due to the capacity of exosomes to play a part in intercellular cell communication. However, the precise role of MSC-derived exosomes and their alterations in immunocompromised pathologies remains unknown, especially in veterinary patients. The goal of this work was to analyze the proteomic profile of feline adipose tissue-derived MSCs (fAd-MSCs) from calicivirus-positive FCGS patients, and to detect possible modifications of the exosomal cargo, to gain better knowledge of the disease's etiopathogenesis. Using high-resolution mass spectrometry and functional enrichment analysis with Gene Ontology, exosomes isolated from the fAd-MSCs of five healthy cats and five calicivirus-positive FCGS patients, were pooled and compared. The results showed that the fAd-MSCs from cats suffering from FCGS not only had a higher exosome production, but also their exosomes showed significant alterations in their proteomic profile. Eight proteins were exclusively found in the exosomes from the FCGS group, and five proteins could only be found in the exosomes from the healthy cats. When comparing the exosomal cargo between the two groups, significant upregulation of 17 and downregulation of 13 proteins were detected in the FCGS group compared to the control group. These findings shed light on new perspectives on the roles of MSCs and their relation to this disease, which may help in identifying new therapeutic targets and selecting specific biomarkers. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021 2021-08-23 2021 2021-08-23 2024 2024-02-19 |
| dc.type.none.fl_str_mv |
research article http://purl.org/coar/resource_type/c_2df8fbb1 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12105/18443 |
| url |
http://hdl.handle.net/20.500.12105/18443 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute (MDPI) |
| publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute (MDPI) |
| dc.source.none.fl_str_mv |
reponame:Repisalud instname:Instituto de Salud Carlos III (ISCIII) |
| instname_str |
Instituto de Salud Carlos III (ISCIII) |
| reponame_str |
Repisalud |
| collection |
Repisalud |
| repository.name.fl_str_mv |
|
| repository.mail.fl_str_mv |
|
| _version_ |
1869408611437903872 |
| spelling |
Altered Proteomic Profile of Adipose Tissue-Derived Mesenchymal Stem Cell Exosomes from Cats with Severe Chronic GingivostomatitisVillatoro, Antonio J.Martín-Astorga, María del CarmenAlcoholado, CristinaCárdenas, CasimiroFariñas, FernandoBecerra, JoséVisser, RickFelineFeline chronic gingivostomatitisMesenchymal stem cellsExosomesUltra-highperformance liquid chromatography high-resolution mass spectrometry (UHPLC–HRMS)Enfermedades de los gatosCélulas madre mesenquimatosasExosomasCromatografía líquida de alta presiónEspectrometría de masasCatsHumansAnimalsExosomesPrevalenceControl GroupsDown-RegulationGene OntologyProteomicsAdipose TissueCaliciviridae InfectionsCell CommunicationMass SpectrometryImmune SystemFeline chronic gingivostomatitis (FCGS) is a pathology with a complicated therapeutic approach and with a prevalence between 0.7 and 12%. Although the etiology of the disease is diverse, feline calicivirus infection is known to be a predisposing factor. To date, the available treatment helps in controlling the disease, but cannot always provide a cure, which leads to a high percentage of refractory animals. Mesenchymal stem cells (MSCs) play a pivotal role in the homeostasis and reparation of different tissues and have the ability to modulate the immune system responses. This ability is, in part, due to the capacity of exosomes to play a part in intercellular cell communication. However, the precise role of MSC-derived exosomes and their alterations in immunocompromised pathologies remains unknown, especially in veterinary patients. The goal of this work was to analyze the proteomic profile of feline adipose tissue-derived MSCs (fAd-MSCs) from calicivirus-positive FCGS patients, and to detect possible modifications of the exosomal cargo, to gain better knowledge of the disease's etiopathogenesis. Using high-resolution mass spectrometry and functional enrichment analysis with Gene Ontology, exosomes isolated from the fAd-MSCs of five healthy cats and five calicivirus-positive FCGS patients, were pooled and compared. The results showed that the fAd-MSCs from cats suffering from FCGS not only had a higher exosome production, but also their exosomes showed significant alterations in their proteomic profile. Eight proteins were exclusively found in the exosomes from the FCGS group, and five proteins could only be found in the exosomes from the healthy cats. When comparing the exosomal cargo between the two groups, significant upregulation of 17 and downregulation of 13 proteins were detected in the FCGS group compared to the control group. These findings shed light on new perspectives on the roles of MSCs and their relation to this disease, which may help in identifying new therapeutic targets and selecting specific biomarkers.Multidisciplinary Digital Publishing Institute (MDPI)[Villatoro,AJ; Martín-Astorga,MC; Alcoholado,C; Becerra,J; Visser,R] Laboratory of Bioengineering and Tissue Regeneration, Department of Cell Biology, Genetics and Physiology, University of Málaga, IBIMA, Málaga, Spain. [Villatoro,AJ] Instituto de Inmunología Clínica y Terapia Celular (IMMUNESTEM), Málaga, Spain. [Cárdenas,C] Research Support Central Services (SCAI) of the University of Málaga, Málaga, Spain. [Fariñas,F] Grupo Ynmun, Spanish Association for the Research in Immunological and Infectious Diseases, Málaga, Spain. [Becerra,J; Visser,R] Biomedical Research Networking Center in Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN), Madrid, Spain. [Becerra,J; Visser,R] Andalusian Centre for Nanomedicine and Biotechnology-University of Málaga (BIONAND), Málaga, Spain.20242024-02-1920212021-08-2320212021-08-23research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articlehttp://hdl.handle.net/20.500.12105/18443reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/184432026-06-12T12:43:37Z |
| score |
15,812429 |