Microsatellite-based genotyping of MHC class II DRB1 gene in Iberian and Alpine ibex

In an analysis of a microsatellite locus (OLADRB1) linked to the MHC DRB1 gene of Iberian and Alpine ibex (Capra pyrenaica and Carpa ibex), we detected strong linkage disequilibrium between both loci. The allele length polymorphism at OLADRB1 was unambiguously linked to a particular DRB1 allele. Thi...

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Detalles Bibliográficos
Autores: Alasaad, Samer, Biebach, Iris, Grossen, Christine, Soriguer, Ramón C., Pérez, Jesús M., Keller, Lukas F.
Tipo de recurso: artículo
Fecha de publicación:2011
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/49467
Acceso en línea:http://hdl.handle.net/10261/49467
Access Level:acceso abierto
Palabra clave:Capra ibex
Capra pyrenaica
OLADRB1
MHC DRB1
Linkage disequilibrium
DRB-STR method
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spelling Microsatellite-based genotyping of MHC class II DRB1 gene in Iberian and Alpine ibexAlasaad, SamerBiebach, IrisGrossen, ChristineSoriguer, Ramón C.Pérez, Jesús M.Keller, Lukas F.Capra ibexCapra pyrenaicaOLADRB1MHC DRB1Linkage disequilibriumDRB-STR methodIn an analysis of a microsatellite locus (OLADRB1) linked to the MHC DRB1 gene of Iberian and Alpine ibex (Capra pyrenaica and Carpa ibex), we detected strong linkage disequilibrium between both loci. The allele length polymorphism at OLADRB1 was unambiguously linked to a particular DRB1 allele. This allowed us to develop a DRB- STR matching method for both ibex species. Validation of the DRB-STR matching method was performed in 160 Iberian ibex from Spain and 98 Alpine ibex from Switzerland and Italy. This simple and relatively inexpensive protocol may find wide applications in a variety of research areas (e.g., mate choice, pathogen-driven selection) and in the biological conservation and management of the Western European ibex populationsPeer reviewedSpringer Nature201220122011info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/49467reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1007/s10344-011-0592-0info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/494672026-05-22T06:33:51Z
dc.title.none.fl_str_mv Microsatellite-based genotyping of MHC class II DRB1 gene in Iberian and Alpine ibex
title Microsatellite-based genotyping of MHC class II DRB1 gene in Iberian and Alpine ibex
spellingShingle Microsatellite-based genotyping of MHC class II DRB1 gene in Iberian and Alpine ibex
Alasaad, Samer
Capra ibex
Capra pyrenaica
OLADRB1
MHC DRB1
Linkage disequilibrium
DRB-STR method
title_short Microsatellite-based genotyping of MHC class II DRB1 gene in Iberian and Alpine ibex
title_full Microsatellite-based genotyping of MHC class II DRB1 gene in Iberian and Alpine ibex
title_fullStr Microsatellite-based genotyping of MHC class II DRB1 gene in Iberian and Alpine ibex
title_full_unstemmed Microsatellite-based genotyping of MHC class II DRB1 gene in Iberian and Alpine ibex
title_sort Microsatellite-based genotyping of MHC class II DRB1 gene in Iberian and Alpine ibex
dc.creator.none.fl_str_mv Alasaad, Samer
Biebach, Iris
Grossen, Christine
Soriguer, Ramón C.
Pérez, Jesús M.
Keller, Lukas F.
author Alasaad, Samer
author_facet Alasaad, Samer
Biebach, Iris
Grossen, Christine
Soriguer, Ramón C.
Pérez, Jesús M.
Keller, Lukas F.
author_role author
author2 Biebach, Iris
Grossen, Christine
Soriguer, Ramón C.
Pérez, Jesús M.
Keller, Lukas F.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Capra ibex
Capra pyrenaica
OLADRB1
MHC DRB1
Linkage disequilibrium
DRB-STR method
topic Capra ibex
Capra pyrenaica
OLADRB1
MHC DRB1
Linkage disequilibrium
DRB-STR method
description In an analysis of a microsatellite locus (OLADRB1) linked to the MHC DRB1 gene of Iberian and Alpine ibex (Capra pyrenaica and Carpa ibex), we detected strong linkage disequilibrium between both loci. The allele length polymorphism at OLADRB1 was unambiguously linked to a particular DRB1 allele. This allowed us to develop a DRB- STR matching method for both ibex species. Validation of the DRB-STR matching method was performed in 160 Iberian ibex from Spain and 98 Alpine ibex from Switzerland and Italy. This simple and relatively inexpensive protocol may find wide applications in a variety of research areas (e.g., mate choice, pathogen-driven selection) and in the biological conservation and management of the Western European ibex populations
publishDate 2011
dc.date.none.fl_str_mv 2011
2012
2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/49467
url http://hdl.handle.net/10261/49467
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1007/s10344-011-0592-0
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.81155

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