Water-soluble carbosilane dendrimers protect phosphorothioate oligonucleotides from binding to serum proteins.
[EN]Treatment of dendriplexes formed between water-soluble carbosilane dendrimers and phosphorothioate oligodeoxynucleotides (ODN) with the anionic detergent sodium dodecyl sulfate disrupted the complexes indicating that the nature of the union in such dendriplexes is merely electrostatic. However,...
| Autores: | , , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2007 |
| País: | España |
| Institución: | Universidad de Salamanca (USAL) |
| Repositorio: | GREDOS. Repositorio Institucional de la Universidad de Salamanca |
| OAI Identifier: | oai:gredos.usal.es:10366/161073 |
| Acceso en línea: | http://hdl.handle.net/10366/161073 |
| Access Level: | acceso abierto |
| Palabra clave: | Drug Carriers Cell Survival Electrophoresis, Agar Gel Enzyme-Linked Immunosorbent Assay Leukocytes, Mononuclear Oligonucleotides Protein Binding Cattle Transfection Humans Serum Albumin Cells Leukocytes Microscopy Electrophoresis Dendrimers Silanes Water Solubility Animals HIV-1 Thionucleotides 3205 Medicina Interna humanos unión proteica portadores de fármacos VIH-1 albúmina sérica enzimoinmunoanálisis por adsorción transfección bovinos agua microscopía leucocitos células animales solubilidad dendrímeros silanos tionucleótidos electroforesis supervivencia celular oligonucleótidos |
| Sumario: | [EN]Treatment of dendriplexes formed between water-soluble carbosilane dendrimers and phosphorothioate oligodeoxynucleotides (ODN) with the anionic detergent sodium dodecyl sulfate disrupted the complexes indicating that the nature of the union in such dendriplexes is merely electrostatic. However, dendriplexes were not dissociated by serum proteins like bovine or human serum albumins, as assessed by gel electrophoresis and fluorescence experiments. This would imply a dendrimer-mediated protective effect able to prevent ODN interactions with serum proteins and additionally could translate into a reduction of the ODN doses needed to achieve the biological effects. The employment of carbosilane dendrimers as carriers may solve the problem of ODN kidnapping by plasmatic proteins as a key drawback for therapeutics involving ODNs. As examples, transfection processes on normal primary peripheral blood cells and diagnosis of HIV infection in the presence of serum have been assayed. |
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