Electrochemical biosensor for aerobic acetate detection

[EN] There is an increasing demand on alternatives methods to animal testing. Numerous health parameters have been already studied using in vitro devices able to mimic the essential functions of the organs, being the real-time monitoring and response to stimuli their main limitations. Regarding the...

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Autores: Forner, E., Ezenarro, J.J., Pérez-Montero, M., Vigués, N., Asensio-Grau, Andrea, Mas, J., Baeza, M., Muñoz-Berbel, X., Villa, R., Gabriel, G., Andrés Grau, Ana María|||0000-0002-6132-3167
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/211981
Acceso en línea:https://riunet.upv.es/handle/10251/211981
Access Level:acceso abierto
Palabra clave:Acetate detection
Alginate hydrogel matrix
Electrochemical biosensor
Escherichia coli
Inkjet printed sensor
Reduced graphene oxide
TECNOLOGIA DE ALIMENTOS
id ES_593e8a70e8b13e4a9ad331ca5a17dcef
oai_identifier_str oai:riunet.upv.es:10251/211981
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Electrochemical biosensor for aerobic acetate detection
title Electrochemical biosensor for aerobic acetate detection
spellingShingle Electrochemical biosensor for aerobic acetate detection
Forner, E.
Acetate detection
Alginate hydrogel matrix
Electrochemical biosensor
Escherichia coli
Inkjet printed sensor
Reduced graphene oxide
TECNOLOGIA DE ALIMENTOS
title_short Electrochemical biosensor for aerobic acetate detection
title_full Electrochemical biosensor for aerobic acetate detection
title_fullStr Electrochemical biosensor for aerobic acetate detection
title_full_unstemmed Electrochemical biosensor for aerobic acetate detection
title_sort Electrochemical biosensor for aerobic acetate detection
dc.creator.none.fl_str_mv Forner, E.
Ezenarro, J.J.
Pérez-Montero, M.
Vigués, N.
Asensio-Grau, Andrea
Mas, J.
Baeza, M.
Muñoz-Berbel, X.
Villa, R.
Gabriel, G.
Andrés Grau, Ana María|||0000-0002-6132-3167
author Forner, E.
author_facet Forner, E.
Ezenarro, J.J.
Pérez-Montero, M.
Vigués, N.
Asensio-Grau, Andrea
Mas, J.
Baeza, M.
Muñoz-Berbel, X.
Villa, R.
Gabriel, G.
Andrés Grau, Ana María|||0000-0002-6132-3167
author_role author
author2 Ezenarro, J.J.
Pérez-Montero, M.
Vigués, N.
Asensio-Grau, Andrea
Mas, J.
Baeza, M.
Muñoz-Berbel, X.
Villa, R.
Gabriel, G.
Andrés Grau, Ana María|||0000-0002-6132-3167
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Departamento de Tecnología de Alimentos
Instituto Universitario de Ingeniería de Alimentos (FoodUPV)
Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural
Generalitat de Catalunya
Agencia Estatal de Investigación
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Acetate detection
Alginate hydrogel matrix
Electrochemical biosensor
Escherichia coli
Inkjet printed sensor
Reduced graphene oxide
TECNOLOGIA DE ALIMENTOS
topic Acetate detection
Alginate hydrogel matrix
Electrochemical biosensor
Escherichia coli
Inkjet printed sensor
Reduced graphene oxide
TECNOLOGIA DE ALIMENTOS
description [EN] There is an increasing demand on alternatives methods to animal testing. Numerous health parameters have been already studied using in vitro devices able to mimic the essential functions of the organs, being the real-time monitoring and response to stimuli their main limitations. Regarding the health of the gut, the short chain fatty acids, and particularly acetate, have emerged as key biomarkers to evaluate gut healthiness and disease development, although the number of acetate biosensors is still very low. This article presents a microbial biosensor based on fully biocompatible materials which is able to detect acetate in aerobic conditions in the range between 11 and 50 mM, and without compromising the viability and function of either bacteria (>90% viability) or mammalian cells (>80% viability). The detection mechanism is based on the metabolism of acetate by Escherichia coli bacteria immobilized on the transducer surface. Ferricyanide is used as a redox mediator to transfer electrons from the acetate metabolism in the bacterial cells to the transducer. High bacterial concentrations are immobilized in the transducer surface (109 cfu mL-1) by electrodeposition of conductive alginate hydrogels doped with reduced graphene oxide. The results show successful outcomes to exploit bacteria as a biosensing tool, based on the use of inkjet printed transducers, biocompatible materials and cell entrapment technologies.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-12-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/211981
url https://riunet.upv.es/handle/10251/211981
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 RTI2018-096786-B-I00 GUT-MICROBIOTA-ON CHIP FOR PRECISION MEDICINE
Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023 PID2021-126253OB-C21 DESARROLLO Y CARACTERIZACION DE BIORREACTORES BASADOS EN H2 Y HERRAMIENTAS DE MONITORIZACION AVANZADAS PARA LA VALORIZACION DE C Y S A PARTIR DE CORRIENTES LIQUIDAS Y GASEOSAS
Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023 PID2021-127653NB-C21 INTEGRACION DE BIOSENSORES, MATRICES DE MICROELECTRODOS 3D Y CIRCUITOS FOTONICOS CON TECNOLOGIA COMPATIBLE CMOS
Generalitat de Catalunya https://doi.org/10.13039/501100002809 2021SGR00495
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento - No comercial - Sin obra derivada (by-nc-nd)
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento - No comercial - Sin obra derivada (by-nc-nd)
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling Electrochemical biosensor for aerobic acetate detectionForner, E.Ezenarro, J.J.Pérez-Montero, M.Vigués, N.Asensio-Grau, AndreaMas, J.Baeza, M.Muñoz-Berbel, X.Villa, R.Gabriel, G.Andrés Grau, Ana María|||0000-0002-6132-3167Acetate detectionAlginate hydrogel matrixElectrochemical biosensorEscherichia coliInkjet printed sensorReduced graphene oxideTECNOLOGIA DE ALIMENTOS[EN] There is an increasing demand on alternatives methods to animal testing. Numerous health parameters have been already studied using in vitro devices able to mimic the essential functions of the organs, being the real-time monitoring and response to stimuli their main limitations. Regarding the health of the gut, the short chain fatty acids, and particularly acetate, have emerged as key biomarkers to evaluate gut healthiness and disease development, although the number of acetate biosensors is still very low. This article presents a microbial biosensor based on fully biocompatible materials which is able to detect acetate in aerobic conditions in the range between 11 and 50 mM, and without compromising the viability and function of either bacteria (>90% viability) or mammalian cells (>80% viability). The detection mechanism is based on the metabolism of acetate by Escherichia coli bacteria immobilized on the transducer surface. Ferricyanide is used as a redox mediator to transfer electrons from the acetate metabolism in the bacterial cells to the transducer. High bacterial concentrations are immobilized in the transducer surface (109 cfu mL-1) by electrodeposition of conductive alginate hydrogels doped with reduced graphene oxide. The results show successful outcomes to exploit bacteria as a biosensing tool, based on the use of inkjet printed transducers, biocompatible materials and cell entrapment technologies.The authors acknowledge the support from the Ministerio de Ciencia Innovacion y Universidades (MICIU/FEDER, EU) , in Spain, through GUMICHIP (RTI2018-096786-B-I00) , GALILEI (PID2021-126253OB-C21) and CHIP4CELL projects (PID2021-127653NB-C21) and the support of the Generalitat de Catalunya to 2021SGR00495. The work has been performed by the ICTS "NANBIOSIS", more specifically by the U8 Unit of the CIBER in Bioengineering, Biomaterials & Nanomedicine (CIBER-BBN) at the IMB-CNM (CSIC) . This work also made use of theSpanish ICTS Network MICRONANOFABS partially supported by MEINCOM. We would like to thank the members of the GENOCOV research group from Universitat Autonoma, specifically Dr. D. Gabriel and PhD student M. Fachal, for their assistance in the liquid chroma-tography analysis presented in this work.ElsevierDepartamento de Tecnología de AlimentosInstituto Universitario de Ingeniería de Alimentos (FoodUPV)Escuela Técnica Superior de Ingeniería Agronómica y del Medio NaturalGeneralitat de CatalunyaAgencia Estatal de InvestigaciónRepositorio Institucional de la Universitat Politècnica de València Riunet20232023-12-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/211981reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 RTI2018-096786-B-I00 GUT-MICROBIOTA-ON CHIP FOR PRECISION MEDICINEAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023 PID2021-126253OB-C21 DESARROLLO Y CARACTERIZACION DE BIORREACTORES BASADOS EN H2 Y HERRAMIENTAS DE MONITORIZACION AVANZADAS PARA LA VALORIZACION DE C Y S A PARTIR DE CORRIENTES LIQUIDAS Y GASEOSASAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023 PID2021-127653NB-C21 INTEGRACION DE BIOSENSORES, MATRICES DE MICROELECTRODOS 3D Y CIRCUITOS FOTONICOS CON TECNOLOGIA COMPATIBLE CMOSGeneralitat de Catalunya https://doi.org/10.13039/501100002809 2021SGR00495open accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/2119812026-06-13T07:49:27Z
score 15.811543

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