A 3D bioprinted hydrogel gut-on-chip with integrated electrodes for transepithelial electrical resistance (TEER) measurements

Conventional gut-on-chip (GOC) models typically represent the epithelial layer of the gut tissue, neglecting other important components such as the stromal compartment and the extracellular matrix (ECM) that play crucial roles in maintaining intestinal barrier integrity and function. These models of...

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Autores: Vera, Daniel, García-Díaz, María, Torras, Núria, Castillo, Óscar, Illa, Xavi, Villa, Rosa, Alvarez, Mar, Castro Martínez, Elena
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/396429
Acceso en línea:http://hdl.handle.net/10261/396429
https://api.elsevier.com/content/abstract/scopus_id/85190563427
Access Level:acceso abierto
Palabra clave:bioprinted
gut-on-a-chip
hydrogels
impedance spectroscopy
integrated electrodes
intestinal barrier
intestinal mucosa
http://metadata.un.org/sdg/9
Build resilient infrastructure, promote inclusive and sustainable industrialization and foster innovation
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dc.title.none.fl_str_mv A 3D bioprinted hydrogel gut-on-chip with integrated electrodes for transepithelial electrical resistance (TEER) measurements
title A 3D bioprinted hydrogel gut-on-chip with integrated electrodes for transepithelial electrical resistance (TEER) measurements
spellingShingle A 3D bioprinted hydrogel gut-on-chip with integrated electrodes for transepithelial electrical resistance (TEER) measurements
Vera, Daniel
bioprinted
gut-on-a-chip
hydrogels
impedance spectroscopy
integrated electrodes
intestinal barrier
intestinal mucosa
http://metadata.un.org/sdg/9
Build resilient infrastructure, promote inclusive and sustainable industrialization and foster innovation
title_short A 3D bioprinted hydrogel gut-on-chip with integrated electrodes for transepithelial electrical resistance (TEER) measurements
title_full A 3D bioprinted hydrogel gut-on-chip with integrated electrodes for transepithelial electrical resistance (TEER) measurements
title_fullStr A 3D bioprinted hydrogel gut-on-chip with integrated electrodes for transepithelial electrical resistance (TEER) measurements
title_full_unstemmed A 3D bioprinted hydrogel gut-on-chip with integrated electrodes for transepithelial electrical resistance (TEER) measurements
title_sort A 3D bioprinted hydrogel gut-on-chip with integrated electrodes for transepithelial electrical resistance (TEER) measurements
dc.creator.none.fl_str_mv Vera, Daniel
García-Díaz, María
Torras, Núria
Castillo, Óscar
Illa, Xavi
Villa, Rosa
Alvarez, Mar
Castro Martínez, Elena
author Vera, Daniel
author_facet Vera, Daniel
García-Díaz, María
Torras, Núria
Castillo, Óscar
Illa, Xavi
Villa, Rosa
Alvarez, Mar
Castro Martínez, Elena
author_role author
author2 García-Díaz, María
Torras, Núria
Castillo, Óscar
Illa, Xavi
Villa, Rosa
Alvarez, Mar
Castro Martínez, Elena
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
European Commission
Centros de Investigación Biomédica en Red (España)
Generalitat de Catalunya
Ministerio de Economía y Competitividad (España)
0000-0002-4794-5437
0000-0001-5027-7428
0000-0003-4590-4401
0000-0002-6585-4213
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv bioprinted
gut-on-a-chip
hydrogels
impedance spectroscopy
integrated electrodes
intestinal barrier
intestinal mucosa
http://metadata.un.org/sdg/9
Build resilient infrastructure, promote inclusive and sustainable industrialization and foster innovation
topic bioprinted
gut-on-a-chip
hydrogels
impedance spectroscopy
integrated electrodes
intestinal barrier
intestinal mucosa
http://metadata.un.org/sdg/9
Build resilient infrastructure, promote inclusive and sustainable industrialization and foster innovation
description Conventional gut-on-chip (GOC) models typically represent the epithelial layer of the gut tissue, neglecting other important components such as the stromal compartment and the extracellular matrix (ECM) that play crucial roles in maintaining intestinal barrier integrity and function. These models often employ hard, flat porous membranes for cell culture, thus failing to recapitulate the soft environment and complex 3D architecture of the intestinal mucosa. Alternatively, hydrogels have been recently introduced in GOCs as ECM analogs to support the co-culture of intestinal cells inin vivo-like configurations, and thus opening new opportunities in the organ-on-chip field. In this work, we present an innovative GOC device that includes a 3D bioprinted hydrogel channel replicating the intestinal villi architecture containing both the epithelial and stromal compartments of the gut mucosa. The bioprinted hydrogels successfully support both the encapsulation of fibroblasts and their co-culture with intestinal epithelial cells under physiological flow conditions. Moreover, we successfully integrated electrodes into the microfluidic system to monitor the barrier formation in real time via transepithelial electrical resistance measurements.
publishDate 2024
dc.date.none.fl_str_mv 2024
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/396429
https://api.elsevier.com/content/abstract/scopus_id/85190563427
url http://hdl.handle.net/10261/396429
https://api.elsevier.com/content/abstract/scopus_id/85190563427
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
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info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-129115OB-I00
info:eu-repo/grantAgreement/EC/H2020/647863
info:eu-repo/grantAgreement/CB06/01/0049
info:eu-repo/grantAgreement/MCIN/IJC2019-040289-I/
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-096786-B-I00
https://iopscience.iop.org/article/10.1088/1758-5090/ad3aa4
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv IOP Publishing
publisher.none.fl_str_mv IOP Publishing
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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spelling A 3D bioprinted hydrogel gut-on-chip with integrated electrodes for transepithelial electrical resistance (TEER) measurementsVera, DanielGarcía-Díaz, MaríaTorras, NúriaCastillo, ÓscarIlla, XaviVilla, RosaAlvarez, MarCastro Martínez, Elenabioprintedgut-on-a-chiphydrogelsimpedance spectroscopyintegrated electrodesintestinal barrierintestinal mucosahttp://metadata.un.org/sdg/9Build resilient infrastructure, promote inclusive and sustainable industrialization and foster innovationConventional gut-on-chip (GOC) models typically represent the epithelial layer of the gut tissue, neglecting other important components such as the stromal compartment and the extracellular matrix (ECM) that play crucial roles in maintaining intestinal barrier integrity and function. These models often employ hard, flat porous membranes for cell culture, thus failing to recapitulate the soft environment and complex 3D architecture of the intestinal mucosa. Alternatively, hydrogels have been recently introduced in GOCs as ECM analogs to support the co-culture of intestinal cells inin vivo-like configurations, and thus opening new opportunities in the organ-on-chip field. In this work, we present an innovative GOC device that includes a 3D bioprinted hydrogel channel replicating the intestinal villi architecture containing both the epithelial and stromal compartments of the gut mucosa. The bioprinted hydrogels successfully support both the encapsulation of fibroblasts and their co-culture with intestinal epithelial cells under physiological flow conditions. Moreover, we successfully integrated electrodes into the microfluidic system to monitor the barrier formation in real time via transepithelial electrical resistance measurements.Funding for this project was provided by a European Union Horizon 2020 ERC Grant (Agreement 647863\u2014COMIET), the CERCA Programme/Generalitat de Catalunya (2017-SGR-1079), and the Spanish Ministry of Economy and Competitiveness (PID2021-129115OB-I00). The authors acknowledge support from the Ministerio de Ciencia Innovacion y Universidades, in Spain, through GUMICHIP project (RTI2018-096786-B-I00 funded by MCIN/AEI/10.13039/501100011033 and by \u2018ERDF A way of making Europe\u2019). D V acknowledge that this work has been done in the framework of the PhD in Electrical and Telecommunication Engineering at the Universitat Aut\u00F2noma de Barcelona. D V was supported by the Marie Sk\u0142odowska-Curie COFUND PhD fellowship (Grant Agreement 754397). N T acknowledges the Spanish Ministry of Science and Innovation, Juan de la Cierva program (Grant IJC2019-040289-I). This research was supported by CIBER -Consorcio Centro de Investigaci\u00F3n Biom\u00E9dica en Red- (CB06/01/0049), Instituto de Salud Carlos III, Ministerio de Ciencia e Innovaci\u00F3n. The results presented here only reflect the views of the authors; the European Commission is not responsible for any use that may be made of the information it contains.Peer reviewedIOP PublishingMinisterio de Ciencia e Innovación (España)European CommissionCentros de Investigación Biomédica en Red (España)Generalitat de CatalunyaMinisterio de Economía y Competitividad (España)0000-0002-4794-54370000-0001-5027-74280000-0003-4590-44010000-0002-6585-4213Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/396429https://api.elsevier.com/content/abstract/scopus_id/85190563427reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-129115OB-I00info:eu-repo/grantAgreement/EC/H2020/647863info:eu-repo/grantAgreement/CB06/01/0049info:eu-repo/grantAgreement/MCIN/IJC2019-040289-I/info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-096786-B-I00https://iopscience.iop.org/article/10.1088/1758-5090/ad3aa4Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3964292026-05-22T06:33:51Z
score 15,811543

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