Human amniotic membrane as newly identifed source of amniotic fuid pulmonary surfactant

Pulmonary surfactant (PS) reduces surface tension at the air-liquid interface in the alveolar epithelium of the lung, which is required for breathing and for the pulmonary maturity of the developing foetus. However, the origin of PS had never been thoroughly investigated, although it was assumed to...

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Autores: Lemke, Angela, Castillo-Sánchez, José Carlos, Prodinger, Florian, Ceranic, Asja, Hennerbichler-Lugscheider, Simone, Pérez-Gil, Jesús, Redl, Heinz, Wolbank, Susanne
Tipo de recurso: artículo
Fecha de publicación:2017
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/18393
Acceso en línea:https://hdl.handle.net/20.500.14352/18393
Access Level:acceso abierto
Palabra clave:577.112
577.2
Amniotic membrane
Pulmonary surfactant
Cell biology
Biología molecular (Biología)
Bioquímica (Biología)
2415 Biología Molecular
2302 Bioquímica
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oai_identifier_str oai:docta.ucm.es:20.500.14352/18393
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network_name_str España
repository_id_str
spelling Human amniotic membrane as newly identifed source of amniotic fuid pulmonary surfactantLemke, AngelaCastillo-Sánchez, José CarlosProdinger, FlorianCeranic, AsjaHennerbichler-Lugscheider, SimonePérez-Gil, JesúsRedl, HeinzWolbank, Susanne577.112577.2Amniotic membranePulmonary surfactantCell biologyBiología molecular (Biología)Bioquímica (Biología)2415 Biología Molecular2302 BioquímicaPulmonary surfactant (PS) reduces surface tension at the air-liquid interface in the alveolar epithelium of the lung, which is required for breathing and for the pulmonary maturity of the developing foetus. However, the origin of PS had never been thoroughly investigated, although it was assumed to be secreted from the foetal developing lung. Human amniotic membrane (hAM), particularly its epithelial cell layer, composes the amniotic sac enclosing the amniotic fuid. In this study, we therefore aimed to investigate a potential contribution of the cellular components of the hAM to pulmonary surfactant found in amniotic fuid. We identifed that cells within the native membrane contain lamellar bodies and express all four surfactant proteins as well as ABCA3. Lipidomic profling by nanoESI – MS/MS revealed the presence of the essential lipid species as found in PS. Also, the biophysical activity of conditioned cell culture supernatant obtained from hAM was tested with captive bubble surfactometry. hAM supernatant showed the ability to reduce surface tension, similar to human PS obtained from bronchoalveolar lavage. This means that hAM produces the essential PS-associated components and can therefore contribute as second potential source of PS in amniotic fuid aside from the foetal lung.Nature Publishing GroupUniversidad Complutense de Madrid20172017-07-2520172017-07-25journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/18393reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 3.0 Españahttps://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/183932026-06-02T12:44:21Z
dc.title.none.fl_str_mv Human amniotic membrane as newly identifed source of amniotic fuid pulmonary surfactant
title Human amniotic membrane as newly identifed source of amniotic fuid pulmonary surfactant
spellingShingle Human amniotic membrane as newly identifed source of amniotic fuid pulmonary surfactant
Lemke, Angela
577.112
577.2
Amniotic membrane
Pulmonary surfactant
Cell biology
Biología molecular (Biología)
Bioquímica (Biología)
2415 Biología Molecular
2302 Bioquímica
title_short Human amniotic membrane as newly identifed source of amniotic fuid pulmonary surfactant
title_full Human amniotic membrane as newly identifed source of amniotic fuid pulmonary surfactant
title_fullStr Human amniotic membrane as newly identifed source of amniotic fuid pulmonary surfactant
title_full_unstemmed Human amniotic membrane as newly identifed source of amniotic fuid pulmonary surfactant
title_sort Human amniotic membrane as newly identifed source of amniotic fuid pulmonary surfactant
dc.creator.none.fl_str_mv Lemke, Angela
Castillo-Sánchez, José Carlos
Prodinger, Florian
Ceranic, Asja
Hennerbichler-Lugscheider, Simone
Pérez-Gil, Jesús
Redl, Heinz
Wolbank, Susanne
author Lemke, Angela
author_facet Lemke, Angela
Castillo-Sánchez, José Carlos
Prodinger, Florian
Ceranic, Asja
Hennerbichler-Lugscheider, Simone
Pérez-Gil, Jesús
Redl, Heinz
Wolbank, Susanne
author_role author
author2 Castillo-Sánchez, José Carlos
Prodinger, Florian
Ceranic, Asja
Hennerbichler-Lugscheider, Simone
Pérez-Gil, Jesús
Redl, Heinz
Wolbank, Susanne
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 577.112
577.2
Amniotic membrane
Pulmonary surfactant
Cell biology
Biología molecular (Biología)
Bioquímica (Biología)
2415 Biología Molecular
2302 Bioquímica
topic 577.112
577.2
Amniotic membrane
Pulmonary surfactant
Cell biology
Biología molecular (Biología)
Bioquímica (Biología)
2415 Biología Molecular
2302 Bioquímica
description Pulmonary surfactant (PS) reduces surface tension at the air-liquid interface in the alveolar epithelium of the lung, which is required for breathing and for the pulmonary maturity of the developing foetus. However, the origin of PS had never been thoroughly investigated, although it was assumed to be secreted from the foetal developing lung. Human amniotic membrane (hAM), particularly its epithelial cell layer, composes the amniotic sac enclosing the amniotic fuid. In this study, we therefore aimed to investigate a potential contribution of the cellular components of the hAM to pulmonary surfactant found in amniotic fuid. We identifed that cells within the native membrane contain lamellar bodies and express all four surfactant proteins as well as ABCA3. Lipidomic profling by nanoESI – MS/MS revealed the presence of the essential lipid species as found in PS. Also, the biophysical activity of conditioned cell culture supernatant obtained from hAM was tested with captive bubble surfactometry. hAM supernatant showed the ability to reduce surface tension, similar to human PS obtained from bronchoalveolar lavage. This means that hAM produces the essential PS-associated components and can therefore contribute as second potential source of PS in amniotic fuid aside from the foetal lung.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-07-25
2017
2017-07-25
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/18393
url https://hdl.handle.net/20.500.14352/18393
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,198674

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