Purification and Identification of Antioxidant Alcalase-Derived Peptides from Sheep Plasma Proteins

In this study, sheep plasma was submitted to Alcalase-hydrolysis and peptides with better antioxidant properties measured through both the ferric-reducing antioxidant power (FRAP) and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability assays were isolated and identified. After hydro...

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Detalles Bibliográficos
Autores: Hou, Chengli, Wu, Liguo, Wang, Zhenyu, Saguer Hom, Elena, Zhang, Dequan
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10256/17255
Acceso en línea:http://hdl.handle.net/10256/17255
Access Level:acceso abierto
Palabra clave:Pèptids
Peptides
Antioxidants
Proteïnes de la sang
Blood proteins
Espectrometria de masses
Mass spectrometry
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spelling Purification and Identification of Antioxidant Alcalase-Derived Peptides from Sheep Plasma ProteinsHou, ChengliWu, LiguoWang, ZhenyuSaguer Hom, ElenaZhang, DequanPèptidsPeptidesAntioxidantsProteïnes de la sangBlood proteinsEspectrometria de massesMass spectrometryIn this study, sheep plasma was submitted to Alcalase-hydrolysis and peptides with better antioxidant properties measured through both the ferric-reducing antioxidant power (FRAP) and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability assays were isolated and identified. After hydrolysate ultrafiltration and semi-preparative reverse-phase high-performance liquid chromatography, nine fractions (F1–F9) were obtained, with the two first (F1 and F2) showing the greatest antioxidant potential. These two fractions were further separated by the AKTA purifier system to generate four (F1-1–F1-4) and five (F2-1–F2-5) fractions, respectively, with two of them (F1-2 and F2-1) exhibiting appreciable FRAP activity and DPPH radical scavenging ability. Using liquid chromatography-tandem mass spectrometry, three antioxidant peptides were identified. From their amino acid sequences (QTALVELLK, SLHTLFGDELCK, and MPCTEDYLSLILNR), which include amino acids that have been previously reported as key contributors to the peptide antioxidant properties, it can be maintained that they come mainly from serum albumin. These results suggested that the sheep plasma protein can be considered as a good source of antioxidant peptides and bring forth new possibilities for the utilization of animal blood by-productsMDPI (Multidisciplinary Digital Publishing Institute)2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer-reviewedapplication/pdfhttp://hdl.handle.net/10256/17255http://hdl.handle.net/10256/17255Antioxidants, 2019, vol. 8, núm. 12, p. 592Articles publicats (D-EQATA)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/semantics/altIdentifier/doi/10.3390/antiox8120592info:eu-repo/semantics/altIdentifier/issn/2076-3921Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:10256/172552026-05-29T05:05:01Z
dc.title.none.fl_str_mv Purification and Identification of Antioxidant Alcalase-Derived Peptides from Sheep Plasma Proteins
title Purification and Identification of Antioxidant Alcalase-Derived Peptides from Sheep Plasma Proteins
spellingShingle Purification and Identification of Antioxidant Alcalase-Derived Peptides from Sheep Plasma Proteins
Hou, Chengli
Pèptids
Peptides
Antioxidants
Proteïnes de la sang
Blood proteins
Espectrometria de masses
Mass spectrometry
title_short Purification and Identification of Antioxidant Alcalase-Derived Peptides from Sheep Plasma Proteins
title_full Purification and Identification of Antioxidant Alcalase-Derived Peptides from Sheep Plasma Proteins
title_fullStr Purification and Identification of Antioxidant Alcalase-Derived Peptides from Sheep Plasma Proteins
title_full_unstemmed Purification and Identification of Antioxidant Alcalase-Derived Peptides from Sheep Plasma Proteins
title_sort Purification and Identification of Antioxidant Alcalase-Derived Peptides from Sheep Plasma Proteins
dc.creator.none.fl_str_mv Hou, Chengli
Wu, Liguo
Wang, Zhenyu
Saguer Hom, Elena
Zhang, Dequan
author Hou, Chengli
author_facet Hou, Chengli
Wu, Liguo
Wang, Zhenyu
Saguer Hom, Elena
Zhang, Dequan
author_role author
author2 Wu, Liguo
Wang, Zhenyu
Saguer Hom, Elena
Zhang, Dequan
author2_role author
author
author
author
dc.subject.none.fl_str_mv Pèptids
Peptides
Antioxidants
Proteïnes de la sang
Blood proteins
Espectrometria de masses
Mass spectrometry
topic Pèptids
Peptides
Antioxidants
Proteïnes de la sang
Blood proteins
Espectrometria de masses
Mass spectrometry
description In this study, sheep plasma was submitted to Alcalase-hydrolysis and peptides with better antioxidant properties measured through both the ferric-reducing antioxidant power (FRAP) and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability assays were isolated and identified. After hydrolysate ultrafiltration and semi-preparative reverse-phase high-performance liquid chromatography, nine fractions (F1–F9) were obtained, with the two first (F1 and F2) showing the greatest antioxidant potential. These two fractions were further separated by the AKTA purifier system to generate four (F1-1–F1-4) and five (F2-1–F2-5) fractions, respectively, with two of them (F1-2 and F2-1) exhibiting appreciable FRAP activity and DPPH radical scavenging ability. Using liquid chromatography-tandem mass spectrometry, three antioxidant peptides were identified. From their amino acid sequences (QTALVELLK, SLHTLFGDELCK, and MPCTEDYLSLILNR), which include amino acids that have been previously reported as key contributors to the peptide antioxidant properties, it can be maintained that they come mainly from serum albumin. These results suggested that the sheep plasma protein can be considered as a good source of antioxidant peptides and bring forth new possibilities for the utilization of animal blood by-products
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
peer-reviewed
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10256/17255
http://hdl.handle.net/10256/17255
url http://hdl.handle.net/10256/17255
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/antiox8120592
info:eu-repo/semantics/altIdentifier/issn/2076-3921
dc.rights.none.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI (Multidisciplinary Digital Publishing Institute)
publisher.none.fl_str_mv MDPI (Multidisciplinary Digital Publishing Institute)
dc.source.none.fl_str_mv Antioxidants, 2019, vol. 8, núm. 12, p. 592
Articles publicats (D-EQATA)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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