Rapid Colorimetric Detection of Wound Infection with a Fluidic Paper Device
Chronic wounds; Infection biomarker; Myeloperoxidase; Colorimetry
| Autores: | , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2022 |
| País: | España |
| Institución: | Departament de Salut de la Generalitat de Catalunya (DS) |
| Repositorio: | Scientia. Dipòsit d'Informació Digital del Departament de Salut |
| OAI Identifier: | oai:scientiasalut.gencat.cat:11351/9583 |
| Acceso en línea: | https://hdl.handle.net/11351/9583 |
| Access Level: | acceso abierto |
| Palabra clave: | Peroxidasa Ferides i lesions Biomarcadors Colorimetria CHEMICALS AND DRUGS::Enzymes and Coenzymes::Enzymes::Oxidoreductases::Peroxidases::Peroxidase DISEASES::Wounds and Injuries CHEMICALS AND DRUGS::Biological Factors::Biomarkers ANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES, AND EQUIPMENT::Investigative Techniques::Chemistry Techniques, Analytical::Titrimetry::Colorimetry COMPUESTOS QUÍMICOS Y DROGAS::enzimas y coenzimas::enzimas::oxidorreductasas::peroxidasas::peroxidasa ENFERMEDADES::heridas y lesiones COMPUESTOS QUÍMICOS Y DROGAS::factores biológicos::biomarcadores TÉCNICAS Y EQUIPOS ANALÍTICOS, DIAGNÓSTICOS Y TERAPÉUTICOS::técnicas de investigación::técnicas de química analítica::volumetría::colorimetría |
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Rapid Colorimetric Detection of Wound Infection with a Fluidic Paper DeviceHoyo, JavierBassegoda, ArnauFerreres Cabanes, GuillemHinojosa-Caballero, DoloresGutiérrez-Capitán, ManuelBaldi, AntonioPeroxidasaFerides i lesionsBiomarcadorsColorimetriaCHEMICALS AND DRUGS::Enzymes and Coenzymes::Enzymes::Oxidoreductases::Peroxidases::PeroxidaseDISEASES::Wounds and InjuriesCHEMICALS AND DRUGS::Biological Factors::BiomarkersANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES, AND EQUIPMENT::Investigative Techniques::Chemistry Techniques, Analytical::Titrimetry::ColorimetryCOMPUESTOS QUÍMICOS Y DROGAS::enzimas y coenzimas::enzimas::oxidorreductasas::peroxidasas::peroxidasaENFERMEDADES::heridas y lesionesCOMPUESTOS QUÍMICOS Y DROGAS::factores biológicos::biomarcadoresTÉCNICAS Y EQUIPOS ANALÍTICOS, DIAGNÓSTICOS Y TERAPÉUTICOS::técnicas de investigación::técnicas de química analítica::volumetría::colorimetríaChronic wounds; Infection biomarker; Myeloperoxidase; ColorimetryFerides i lesions; Biomarcadors d'infecció; Mieloperoxidasa; ColorimetriaHeridas crónicas; Biomarcador de infección; Mieloperoxidasa; ColorimetríaCurrent procedures for the assessment of chronic wound infection are time-consuming and require complex instruments and trained personnel. The incidence of chronic wounds worldwide, and the associated economic burden, urge for simple and cheap point-of-care testing (PoCT) devices for fast on-site diagnosis to enable appropriate early treatment. The enzyme myeloperoxidase (MPO), whose activity in infected wounds is about ten times higher than in non-infected wounds, appears to be a suitable biomarker for wound infection diagnosis. Herein, we develop a single-component foldable paper-based device for the detection of MPO in wound fluids. The analyte detection is achieved in two steps: (i) selective immunocapture of MPO, and (ii) reaction of a specific dye with the captured MPO, yielding a purple color with increasing intensity as a function of the MPO activity in infected wounds in the range of 20-85 U/mL. Ex vivo experiments with wound fluids validated the analytic efficiency of the paper-based device, and the results strongly correlate with a spectrophotometric assay.MDPI[Hoyo J, Bassegoda A, Ferreres G] Grup de Biotecnologia Molecular i Industrial, Departament d'Enginyeria Química, Universitat Politècnica de Catalunya, Terrassa, Spain. [Hinojosa-Caballero D] Unitat de Ferides Complexes, Consorci Sanitari de Terrassa, Hospital de Terrassa, Terrassa, Spain. [Gutiérrez-Capitán M, Baldi A] Instituto de Microelectrónica de Barcelona (IMB-CNM), CSIC, Campus UAB, Bellaterra, SpainConsorci Sanitari de Terrassa202320232022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/11351/9583Scientiareponame:Scientia. Dipòsit d'Informació Digital del Departament de Salutinstname:Departament de Salut de la Generalitat de Catalunya (DS)InglésInternational Journal of Molecular Sciences;23(16)https://doi.org/10.3390/ijms23169129Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:scientiasalut.gencat.cat:11351/95832026-06-12T09:38:37Z |
| dc.title.none.fl_str_mv |
Rapid Colorimetric Detection of Wound Infection with a Fluidic Paper Device |
| title |
Rapid Colorimetric Detection of Wound Infection with a Fluidic Paper Device |
| spellingShingle |
Rapid Colorimetric Detection of Wound Infection with a Fluidic Paper Device Hoyo, Javier Peroxidasa Ferides i lesions Biomarcadors Colorimetria CHEMICALS AND DRUGS::Enzymes and Coenzymes::Enzymes::Oxidoreductases::Peroxidases::Peroxidase DISEASES::Wounds and Injuries CHEMICALS AND DRUGS::Biological Factors::Biomarkers ANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES, AND EQUIPMENT::Investigative Techniques::Chemistry Techniques, Analytical::Titrimetry::Colorimetry COMPUESTOS QUÍMICOS Y DROGAS::enzimas y coenzimas::enzimas::oxidorreductasas::peroxidasas::peroxidasa ENFERMEDADES::heridas y lesiones COMPUESTOS QUÍMICOS Y DROGAS::factores biológicos::biomarcadores TÉCNICAS Y EQUIPOS ANALÍTICOS, DIAGNÓSTICOS Y TERAPÉUTICOS::técnicas de investigación::técnicas de química analítica::volumetría::colorimetría |
| title_short |
Rapid Colorimetric Detection of Wound Infection with a Fluidic Paper Device |
| title_full |
Rapid Colorimetric Detection of Wound Infection with a Fluidic Paper Device |
| title_fullStr |
Rapid Colorimetric Detection of Wound Infection with a Fluidic Paper Device |
| title_full_unstemmed |
Rapid Colorimetric Detection of Wound Infection with a Fluidic Paper Device |
| title_sort |
Rapid Colorimetric Detection of Wound Infection with a Fluidic Paper Device |
| dc.creator.none.fl_str_mv |
Hoyo, Javier Bassegoda, Arnau Ferreres Cabanes, Guillem Hinojosa-Caballero, Dolores Gutiérrez-Capitán, Manuel Baldi, Antonio |
| author |
Hoyo, Javier |
| author_facet |
Hoyo, Javier Bassegoda, Arnau Ferreres Cabanes, Guillem Hinojosa-Caballero, Dolores Gutiérrez-Capitán, Manuel Baldi, Antonio |
| author_role |
author |
| author2 |
Bassegoda, Arnau Ferreres Cabanes, Guillem Hinojosa-Caballero, Dolores Gutiérrez-Capitán, Manuel Baldi, Antonio |
| author2_role |
author author author author author |
| dc.contributor.none.fl_str_mv |
[Hoyo J, Bassegoda A, Ferreres G] Grup de Biotecnologia Molecular i Industrial, Departament d'Enginyeria Química, Universitat Politècnica de Catalunya, Terrassa, Spain. [Hinojosa-Caballero D] Unitat de Ferides Complexes, Consorci Sanitari de Terrassa, Hospital de Terrassa, Terrassa, Spain. [Gutiérrez-Capitán M, Baldi A] Instituto de Microelectrónica de Barcelona (IMB-CNM), CSIC, Campus UAB, Bellaterra, Spain Consorci Sanitari de Terrassa |
| dc.subject.none.fl_str_mv |
Peroxidasa Ferides i lesions Biomarcadors Colorimetria CHEMICALS AND DRUGS::Enzymes and Coenzymes::Enzymes::Oxidoreductases::Peroxidases::Peroxidase DISEASES::Wounds and Injuries CHEMICALS AND DRUGS::Biological Factors::Biomarkers ANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES, AND EQUIPMENT::Investigative Techniques::Chemistry Techniques, Analytical::Titrimetry::Colorimetry COMPUESTOS QUÍMICOS Y DROGAS::enzimas y coenzimas::enzimas::oxidorreductasas::peroxidasas::peroxidasa ENFERMEDADES::heridas y lesiones COMPUESTOS QUÍMICOS Y DROGAS::factores biológicos::biomarcadores TÉCNICAS Y EQUIPOS ANALÍTICOS, DIAGNÓSTICOS Y TERAPÉUTICOS::técnicas de investigación::técnicas de química analítica::volumetría::colorimetría |
| topic |
Peroxidasa Ferides i lesions Biomarcadors Colorimetria CHEMICALS AND DRUGS::Enzymes and Coenzymes::Enzymes::Oxidoreductases::Peroxidases::Peroxidase DISEASES::Wounds and Injuries CHEMICALS AND DRUGS::Biological Factors::Biomarkers ANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES, AND EQUIPMENT::Investigative Techniques::Chemistry Techniques, Analytical::Titrimetry::Colorimetry COMPUESTOS QUÍMICOS Y DROGAS::enzimas y coenzimas::enzimas::oxidorreductasas::peroxidasas::peroxidasa ENFERMEDADES::heridas y lesiones COMPUESTOS QUÍMICOS Y DROGAS::factores biológicos::biomarcadores TÉCNICAS Y EQUIPOS ANALÍTICOS, DIAGNÓSTICOS Y TERAPÉUTICOS::técnicas de investigación::técnicas de química analítica::volumetría::colorimetría |
| description |
Chronic wounds; Infection biomarker; Myeloperoxidase; Colorimetry |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022 2023 2023 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/11351/9583 |
| url |
https://hdl.handle.net/11351/9583 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
International Journal of Molecular Sciences;23(16) https://doi.org/10.3390/ijms23169129 |
| dc.rights.none.fl_str_mv |
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ |
| eu_rights_str_mv |
openAccess |
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application/pdf |
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MDPI |
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MDPI |
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Scientia reponame:Scientia. Dipòsit d'Informació Digital del Departament de Salut instname:Departament de Salut de la Generalitat de Catalunya (DS) |
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Departament de Salut de la Generalitat de Catalunya (DS) |
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Scientia. Dipòsit d'Informació Digital del Departament de Salut |
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Scientia. Dipòsit d'Informació Digital del Departament de Salut |
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15,811543 |