Overexpression of P2X3 and P2X7 Receptors and TRPV1 Channels in Adrenomedullary Chromaffin Cells in a Rat Model of Neuropathic Pain

We have tested the hypothesis that neuropathic pain acting as a stressor drives functional plasticity in the sympathoadrenal system. The relation between neuropathic pain and adrenal medulla function was studied with behavioral, immunohistochemical and electrophysiological techniques in rats subject...

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Authors: Arribas Blázquez, Marina, Olivos Ore, Luis Alcides, Barahona Gomáriz, María Victoria, Sánchez de la Muela, Mercedes, Solar, Virginia, Jiménez, Esperanza, Gualix Sánchez, Francisco Javier, McIntosh, J. Michael, Ferrer-Montiel, Antonio, Miras Portugal, María Teresa, Rodríguez Artalejo, Antonio
Format: article
Publication Date:2019
Country:España
Institution:Universidad Complutense de Madrid (UCM)
Repository:Docta Complutense
Language:English
OAI Identifier:oai:docta.ucm.es:20.500.14352/12515
Online Access:https://hdl.handle.net/20.500.14352/12515
Access Level:Open access
Keyword:P2X3 receptors
P2X7 receptors
TRPV1 channels
α9 nicotinic acetylcholine receptors
neuropathic pain
chromaffin cells
adrenal medulla
stress
Microbiología (Veterinaria)
Patología veterinaria
3109.05 Microbiología
3109.07 Patología
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oai_identifier_str oai:docta.ucm.es:20.500.14352/12515
network_acronym_str ES
network_name_str España
repository_id_str
spelling Overexpression of P2X3 and P2X7 Receptors and TRPV1 Channels in Adrenomedullary Chromaffin Cells in a Rat Model of Neuropathic PainArribas Blázquez, MarinaOlivos Ore, Luis AlcidesBarahona Gomáriz, María VictoriaSánchez de la Muela, MercedesSolar, VirginiaJiménez, EsperanzaGualix Sánchez, Francisco JavierMcIntosh, J. MichaelFerrer-Montiel, AntonioMiras Portugal, María TeresaRodríguez Artalejo, AntonioP2X3 receptorsP2X7 receptorsTRPV1 channelsα9 nicotinic acetylcholine receptorsneuropathic painchromaffin cellsadrenal medullastressMicrobiología (Veterinaria)Patología veterinaria3109.05 Microbiología3109.07 PatologíaWe have tested the hypothesis that neuropathic pain acting as a stressor drives functional plasticity in the sympathoadrenal system. The relation between neuropathic pain and adrenal medulla function was studied with behavioral, immunohistochemical and electrophysiological techniques in rats subjected to chronic constriction injury of the sciatic nerve. In slices of the adrenal gland from neuropathic animals, we have evidenced increased cholinergic innervation and spontaneous synaptic activity at the splanchnic nerve–chromaffin cell junction. Likewise, adrenomedullary chromaffin cells displayed enlarged acetylcholine-evoked currents with greater sensitivity to α-conotoxin RgIA, a selective blocker of α9 subunit-containing nicotinic acetylcholine receptors, as well as increased exocytosis triggered by voltage-activated Ca2+ entry. Altogether, these adaptations are expected to facilitate catecholamine output into the bloodstream. Last, but most intriguing, functional and immunohistochemical data indicate that P2X3 and P2X7 purinergic receptors and transient receptor potential vanilloid-1 (TRPV1) channels are overexpressed in chromaffin cells from neuropathic animals. These latter observations are reminiscent of molecular changes characteristic of peripheral sensitization of nociceptors following the lesion of a peripheral nerve, and suggest that similar phenomena can occur in other tissues, potentially contributing to behavioral manifestations of neuropathic pain.MDPIUniversidad Complutense de Madrid20192019-01-0320192019-01-03journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/12515reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 3.0 Españahttps://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/125152026-06-02T12:44:21Z
dc.title.none.fl_str_mv Overexpression of P2X3 and P2X7 Receptors and TRPV1 Channels in Adrenomedullary Chromaffin Cells in a Rat Model of Neuropathic Pain
title Overexpression of P2X3 and P2X7 Receptors and TRPV1 Channels in Adrenomedullary Chromaffin Cells in a Rat Model of Neuropathic Pain
spellingShingle Overexpression of P2X3 and P2X7 Receptors and TRPV1 Channels in Adrenomedullary Chromaffin Cells in a Rat Model of Neuropathic Pain
Arribas Blázquez, Marina
P2X3 receptors
P2X7 receptors
TRPV1 channels
α9 nicotinic acetylcholine receptors
neuropathic pain
chromaffin cells
adrenal medulla
stress
Microbiología (Veterinaria)
Patología veterinaria
3109.05 Microbiología
3109.07 Patología
title_short Overexpression of P2X3 and P2X7 Receptors and TRPV1 Channels in Adrenomedullary Chromaffin Cells in a Rat Model of Neuropathic Pain
title_full Overexpression of P2X3 and P2X7 Receptors and TRPV1 Channels in Adrenomedullary Chromaffin Cells in a Rat Model of Neuropathic Pain
title_fullStr Overexpression of P2X3 and P2X7 Receptors and TRPV1 Channels in Adrenomedullary Chromaffin Cells in a Rat Model of Neuropathic Pain
title_full_unstemmed Overexpression of P2X3 and P2X7 Receptors and TRPV1 Channels in Adrenomedullary Chromaffin Cells in a Rat Model of Neuropathic Pain
title_sort Overexpression of P2X3 and P2X7 Receptors and TRPV1 Channels in Adrenomedullary Chromaffin Cells in a Rat Model of Neuropathic Pain
dc.creator.none.fl_str_mv Arribas Blázquez, Marina
Olivos Ore, Luis Alcides
Barahona Gomáriz, María Victoria
Sánchez de la Muela, Mercedes
Solar, Virginia
Jiménez, Esperanza
Gualix Sánchez, Francisco Javier
McIntosh, J. Michael
Ferrer-Montiel, Antonio
Miras Portugal, María Teresa
Rodríguez Artalejo, Antonio
author Arribas Blázquez, Marina
author_facet Arribas Blázquez, Marina
Olivos Ore, Luis Alcides
Barahona Gomáriz, María Victoria
Sánchez de la Muela, Mercedes
Solar, Virginia
Jiménez, Esperanza
Gualix Sánchez, Francisco Javier
McIntosh, J. Michael
Ferrer-Montiel, Antonio
Miras Portugal, María Teresa
Rodríguez Artalejo, Antonio
author_role author
author2 Olivos Ore, Luis Alcides
Barahona Gomáriz, María Victoria
Sánchez de la Muela, Mercedes
Solar, Virginia
Jiménez, Esperanza
Gualix Sánchez, Francisco Javier
McIntosh, J. Michael
Ferrer-Montiel, Antonio
Miras Portugal, María Teresa
Rodríguez Artalejo, Antonio
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv P2X3 receptors
P2X7 receptors
TRPV1 channels
α9 nicotinic acetylcholine receptors
neuropathic pain
chromaffin cells
adrenal medulla
stress
Microbiología (Veterinaria)
Patología veterinaria
3109.05 Microbiología
3109.07 Patología
topic P2X3 receptors
P2X7 receptors
TRPV1 channels
α9 nicotinic acetylcholine receptors
neuropathic pain
chromaffin cells
adrenal medulla
stress
Microbiología (Veterinaria)
Patología veterinaria
3109.05 Microbiología
3109.07 Patología
description We have tested the hypothesis that neuropathic pain acting as a stressor drives functional plasticity in the sympathoadrenal system. The relation between neuropathic pain and adrenal medulla function was studied with behavioral, immunohistochemical and electrophysiological techniques in rats subjected to chronic constriction injury of the sciatic nerve. In slices of the adrenal gland from neuropathic animals, we have evidenced increased cholinergic innervation and spontaneous synaptic activity at the splanchnic nerve–chromaffin cell junction. Likewise, adrenomedullary chromaffin cells displayed enlarged acetylcholine-evoked currents with greater sensitivity to α-conotoxin RgIA, a selective blocker of α9 subunit-containing nicotinic acetylcholine receptors, as well as increased exocytosis triggered by voltage-activated Ca2+ entry. Altogether, these adaptations are expected to facilitate catecholamine output into the bloodstream. Last, but most intriguing, functional and immunohistochemical data indicate that P2X3 and P2X7 purinergic receptors and transient receptor potential vanilloid-1 (TRPV1) channels are overexpressed in chromaffin cells from neuropathic animals. These latter observations are reminiscent of molecular changes characteristic of peripheral sensitization of nociceptors following the lesion of a peripheral nerve, and suggest that similar phenomena can occur in other tissues, potentially contributing to behavioral manifestations of neuropathic pain.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-01-03
2019
2019-01-03
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/12515
url https://hdl.handle.net/20.500.14352/12515
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869408542400708608
score 15,300724

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