Differential Expression of MicroRNAs in Silent and Functioning

The potential role of miRNAs in the silencing mechanisms of pituitary neuroendocrine tumors (PitNETs) has not been addressed. The aim of the present study was to evaluate the expression levels and the potential associated role of some miRNAs, pathways, and transcription factors in the silencing mech...

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Detalhes bibliográficos
Autores: García-Martínez, Araceli, Fuentes-Fayos, Antonio C., Fajardo Montañana, Carmen, Lamas, Cristina, Cámara, Rosa, López-Muñoz, Beatriz, Aranda, Ignacio, Luque, Raúl M., Picó, Antonio
Formato: artículo
Fecha de publicación:2020
País:España
Recursos:Universidad Católica de Valencia San Vicente Mártir
Repositorio:RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártir
Idioma:inglés
OAI Identifier:oai:riucv.ucv.es:20.500.12466/7504
Acesso em linha:https://hdl.handle.net/20.500.12466/7504
Access Level:acceso abierto
Palavra-chave:Pituitary neuroendocrine tumors
Corticotroph tumor
miRNA
Transcriptional regulation
32 Ciencias Médicas
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repository_id_str
spelling Differential Expression of MicroRNAs in Silent and FunctioningGarcía-Martínez, AraceliFuentes-Fayos, Antonio C.Fajardo Montañana, CarmenLamas, CristinaCámara, RosaLópez-Muñoz, BeatrizAranda, IgnacioLuque, Raúl M.Picó, AntonioPituitary neuroendocrine tumorsCorticotroph tumormiRNATranscriptional regulation32 Ciencias MédicasThe potential role of miRNAs in the silencing mechanisms of pituitary neuroendocrine tumors (PitNETs) has not been addressed. The aim of the present study was to evaluate the expression levels and the potential associated role of some miRNAs, pathways, and transcription factors in the silencing mechanisms of corticotroph tumors (CTs). Accordingly, the expression of miR-375, miR-383, miR-488, miR-200a and miR-103; of PKA, MAP3K8, MEK, MAPK3, NGFIB, NURR1, PITX1, and STAT3 were analyzed via qRT-PCR in 23 silent and 24 functioning CTs. miR-200a and miR-103 showed significantly higher expression in silent than in functioning CTs, even after eliminating the bias of tumor size, therefore enabling the differentiation between the two variants. Additionally, miR-383 correlated negatively with TBX19 in silent CTs, a transcription factor related with the processing of POMC that can participate in the silencing mechanisms of CTs. Finally, the gene expression levels of miR-488, miR-200a, and miR-103 were significantly higher in macroadenomas (functioning and silent) than in microadenomas. The evidence from this study indicates that miRNAs could be involved in the pathophysiology of CTs. The translational implications of these findings suggest that pharmacological treatments specifically targeting these miRNAs could become a promising therapeutic option for these patients.20262026-02-1320202020-06-1220202020-06-12journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.12466/7504reponame:RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártirinstname:Universidad Católica de Valencia San Vicente MártirInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:riucv.ucv.es:20.500.12466/75042026-06-19T08:32:07Z
dc.title.none.fl_str_mv Differential Expression of MicroRNAs in Silent and Functioning
title Differential Expression of MicroRNAs in Silent and Functioning
spellingShingle Differential Expression of MicroRNAs in Silent and Functioning
García-Martínez, Araceli
Pituitary neuroendocrine tumors
Corticotroph tumor
miRNA
Transcriptional regulation
32 Ciencias Médicas
title_short Differential Expression of MicroRNAs in Silent and Functioning
title_full Differential Expression of MicroRNAs in Silent and Functioning
title_fullStr Differential Expression of MicroRNAs in Silent and Functioning
title_full_unstemmed Differential Expression of MicroRNAs in Silent and Functioning
title_sort Differential Expression of MicroRNAs in Silent and Functioning
dc.creator.none.fl_str_mv García-Martínez, Araceli
Fuentes-Fayos, Antonio C.
Fajardo Montañana, Carmen
Lamas, Cristina
Cámara, Rosa
López-Muñoz, Beatriz
Aranda, Ignacio
Luque, Raúl M.
Picó, Antonio
author García-Martínez, Araceli
author_facet García-Martínez, Araceli
Fuentes-Fayos, Antonio C.
Fajardo Montañana, Carmen
Lamas, Cristina
Cámara, Rosa
López-Muñoz, Beatriz
Aranda, Ignacio
Luque, Raúl M.
Picó, Antonio
author_role author
author2 Fuentes-Fayos, Antonio C.
Fajardo Montañana, Carmen
Lamas, Cristina
Cámara, Rosa
López-Muñoz, Beatriz
Aranda, Ignacio
Luque, Raúl M.
Picó, Antonio
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv Pituitary neuroendocrine tumors
Corticotroph tumor
miRNA
Transcriptional regulation
32 Ciencias Médicas
topic Pituitary neuroendocrine tumors
Corticotroph tumor
miRNA
Transcriptional regulation
32 Ciencias Médicas
description The potential role of miRNAs in the silencing mechanisms of pituitary neuroendocrine tumors (PitNETs) has not been addressed. The aim of the present study was to evaluate the expression levels and the potential associated role of some miRNAs, pathways, and transcription factors in the silencing mechanisms of corticotroph tumors (CTs). Accordingly, the expression of miR-375, miR-383, miR-488, miR-200a and miR-103; of PKA, MAP3K8, MEK, MAPK3, NGFIB, NURR1, PITX1, and STAT3 were analyzed via qRT-PCR in 23 silent and 24 functioning CTs. miR-200a and miR-103 showed significantly higher expression in silent than in functioning CTs, even after eliminating the bias of tumor size, therefore enabling the differentiation between the two variants. Additionally, miR-383 correlated negatively with TBX19 in silent CTs, a transcription factor related with the processing of POMC that can participate in the silencing mechanisms of CTs. Finally, the gene expression levels of miR-488, miR-200a, and miR-103 were significantly higher in macroadenomas (functioning and silent) than in microadenomas. The evidence from this study indicates that miRNAs could be involved in the pathophysiology of CTs. The translational implications of these findings suggest that pharmacological treatments specifically targeting these miRNAs could become a promising therapeutic option for these patients.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-06-12
2020
2020-06-12
2026
2026-02-13
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.12466/7504
url https://hdl.handle.net/20.500.12466/7504
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártir
instname:Universidad Católica de Valencia San Vicente Mártir
instname_str Universidad Católica de Valencia San Vicente Mártir
reponame_str RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártir
collection RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártir
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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