Proteomic characterization of primary cultured myocytes in a fish model at different myogenesis stages

Myogenesis is a complex two-phase process of proliferation and differentiation, which seems to be greatly conserved in vertebrates. For the first time in fish, we identify the changes that occur in the proteome during this process in a gilthead sea bream (Sparus aurata) myocyte primary cell culture...

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Autores: Millan-Cubillo, Antonio F., Martín Pérez, Miguel, Ibarz i Valls, Antoni, Fernández Borrás, J. (Jaume), Gutiérrez Fruitós, Joaquín, Blasco Mínguez, Josefina
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/151166
Acceso en línea:https://hdl.handle.net/2445/151166
Access Level:acceso abierto
Palabra clave:Miogènesi
Peixos
Myogenesis
Fishes
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spelling Proteomic characterization of primary cultured myocytes in a fish model at different myogenesis stagesMillan-Cubillo, Antonio F.Martín Pérez, MiguelIbarz i Valls, AntoniFernández Borrás, J. (Jaume)Gutiérrez Fruitós, JoaquínBlasco Mínguez, JosefinaMiogènesiPeixosMyogenesisFishesMyogenesis is a complex two-phase process of proliferation and differentiation, which seems to be greatly conserved in vertebrates. For the first time in fish, we identify the changes that occur in the proteome during this process in a gilthead sea bream (Sparus aurata) myocyte primary cell culture (on days 4, 8 and 12), using 2-D gel electrophoresis and LC-MS/MS. A significant increase of myogenin expression at day 8 marked the transition from proliferation to differentiation. Of the 898 spots in the proteome analysis, the 25 protein spots overexpressed on day 4 and the 15 protein spots overexpressed on day 8 indicate the end of proliferation and the beginning of differentiation, respectively. Proliferation was characterized by enrichment of proteins involved in actin cytoskeleton remodelling and in cellular metabolic processes (transcription, ubiquitination, response to stress and glucose metabolism). During differentiation, 41 proteins were overexpressed and 51 underexpressed; many of them related to biosynthetic processes (RNA and protein synthesis and folding, and pentose pathways), terminal myotube formation and muscle contraction. The main cellular processes of both phases of muscle development in fish are similar with those observed in mammals but extended in time, allowing sequential studies of myogenesis.Nature Publishing Group2020202020192020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion16 p.application/pdfapplication/pdfhttps://hdl.handle.net/2445/151166Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1038/s41598-019-50651-wScientific Reports, 2019, vol. 9, p. 14126https://doi.org/10.1038/s41598-019-50651-wcc-by (c) Millan-Cubillo, Antonio F. et al., 2019http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/1511662026-05-29T05:05:01Z
dc.title.none.fl_str_mv Proteomic characterization of primary cultured myocytes in a fish model at different myogenesis stages
title Proteomic characterization of primary cultured myocytes in a fish model at different myogenesis stages
spellingShingle Proteomic characterization of primary cultured myocytes in a fish model at different myogenesis stages
Millan-Cubillo, Antonio F.
Miogènesi
Peixos
Myogenesis
Fishes
title_short Proteomic characterization of primary cultured myocytes in a fish model at different myogenesis stages
title_full Proteomic characterization of primary cultured myocytes in a fish model at different myogenesis stages
title_fullStr Proteomic characterization of primary cultured myocytes in a fish model at different myogenesis stages
title_full_unstemmed Proteomic characterization of primary cultured myocytes in a fish model at different myogenesis stages
title_sort Proteomic characterization of primary cultured myocytes in a fish model at different myogenesis stages
dc.creator.none.fl_str_mv Millan-Cubillo, Antonio F.
Martín Pérez, Miguel
Ibarz i Valls, Antoni
Fernández Borrás, J. (Jaume)
Gutiérrez Fruitós, Joaquín
Blasco Mínguez, Josefina
author Millan-Cubillo, Antonio F.
author_facet Millan-Cubillo, Antonio F.
Martín Pérez, Miguel
Ibarz i Valls, Antoni
Fernández Borrás, J. (Jaume)
Gutiérrez Fruitós, Joaquín
Blasco Mínguez, Josefina
author_role author
author2 Martín Pérez, Miguel
Ibarz i Valls, Antoni
Fernández Borrás, J. (Jaume)
Gutiérrez Fruitós, Joaquín
Blasco Mínguez, Josefina
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Miogènesi
Peixos
Myogenesis
Fishes
topic Miogènesi
Peixos
Myogenesis
Fishes
description Myogenesis is a complex two-phase process of proliferation and differentiation, which seems to be greatly conserved in vertebrates. For the first time in fish, we identify the changes that occur in the proteome during this process in a gilthead sea bream (Sparus aurata) myocyte primary cell culture (on days 4, 8 and 12), using 2-D gel electrophoresis and LC-MS/MS. A significant increase of myogenin expression at day 8 marked the transition from proliferation to differentiation. Of the 898 spots in the proteome analysis, the 25 protein spots overexpressed on day 4 and the 15 protein spots overexpressed on day 8 indicate the end of proliferation and the beginning of differentiation, respectively. Proliferation was characterized by enrichment of proteins involved in actin cytoskeleton remodelling and in cellular metabolic processes (transcription, ubiquitination, response to stress and glucose metabolism). During differentiation, 41 proteins were overexpressed and 51 underexpressed; many of them related to biosynthetic processes (RNA and protein synthesis and folding, and pentose pathways), terminal myotube formation and muscle contraction. The main cellular processes of both phases of muscle development in fish are similar with those observed in mammals but extended in time, allowing sequential studies of myogenesis.
publishDate 2019
dc.date.none.fl_str_mv 2019
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/151166
url https://hdl.handle.net/2445/151166
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1038/s41598-019-50651-w
Scientific Reports, 2019, vol. 9, p. 14126
https://doi.org/10.1038/s41598-019-50651-w
dc.rights.none.fl_str_mv cc-by (c) Millan-Cubillo, Antonio F. et al., 2019
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Millan-Cubillo, Antonio F. et al., 2019
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 16 p.
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.81155

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