Early sepsis diagnosis via protein and miRNA biomarkers using a novel point-of-care photonic biosensor

Sepsis is a condition characterized by a severe stage of blood-infection often leading to tissue damage, organ failure and finally death. Fast diagnosis and identification of the sepsis stage (sepsis, severe sepsis or septic shock) is critical for the patient's evolution and could help in defin...

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Autores: Fabri Faja, Nuria|||0000-0002-6778-8467, Calvo Lozano, Olalla|||0000-0001-5486-2237, Dey, Priyanka|||0000-0002-7158-4491, Terborg, Roland A., Estévez, M. Carmen|||0000-0003-3694-7186, Belushkin, Alexander|||0000-0003-1928-2223, Yesilköy, Filiz|||0000-0001-8483-3285, Duempelmann, Luc, Altug, Hatice|||0000-0001-5522-1343, Pruneri, Valerio, Lechuga, Laura M.|||0000-0001-5187-5358
Formato: artículo
Fecha de publicación:2019
País:España
Recursos:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:210797
Acesso em linha:https://ddd.uab.cat/record/210797
https://dx.doi.org/urn:doi:10.1016/j.aca.2019.05.038
Access Level:acceso abierto
Palavra-chave:Point-of-care
Label-free multiplexed biosensing
Nanoplasmonics
Sepsis
Protein and miRNA biomarkers
Microarray
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spelling Early sepsis diagnosis via protein and miRNA biomarkers using a novel point-of-care photonic biosensorFabri Faja, Nuria|||0000-0002-6778-8467Calvo Lozano, Olalla|||0000-0001-5486-2237Dey, Priyanka|||0000-0002-7158-4491Terborg, Roland A.Estévez, M. Carmen|||0000-0003-3694-7186Belushkin, Alexander|||0000-0003-1928-2223Yesilköy, Filiz|||0000-0001-8483-3285Duempelmann, LucAltug, Hatice|||0000-0001-5522-1343Pruneri, ValerioLechuga, Laura M.|||0000-0001-5187-5358Point-of-careLabel-free multiplexed biosensingNanoplasmonicsSepsisProtein and miRNA biomarkersMicroarraySepsis is a condition characterized by a severe stage of blood-infection often leading to tissue damage, organ failure and finally death. Fast diagnosis and identification of the sepsis stage (sepsis, severe sepsis or septic shock) is critical for the patient's evolution and could help in defining the most adequate treatment in order to reduce its mortality. The combined detection of several biomarkers in a timely, specific and simultaneous way could ensure a more accurate diagnosis. We have designed a new optical point-of-care (POC) device based on a phase-sensitive interferometric biosensor with a label-free microarray configuration for potential high-throughput evaluation of specific sepsis biomarkers. The sensor chip, which relies on the use of metallic nanostructures, provides versatility in terms of biofunctionalization, allowing the efficient immobilization of different kind of receptors such as antibodies or oligonucleotides. We have focused on two structurally different types of biomarkers: proteins, including C-reactive protein (CRP) and Interleukin 6 (IL6), and miRNAs, using miRNA-16 as an example. Limits of Detection (LoD) of 18 μg mL, 88 μg mL and 1 μM (6 μg mL) have been respectively obtained for CRP, IL6 and miRNA-16 in individual assays, with high accuracy and reproducibility. The multiplexing capabilities have also been assessed with the simultaneous analysis of both protein biomarkers. 22019-01-0120192019-01-01Articlehttp://purl.org/coar/resource_type/c_6501SMURhttp://purl.org/coar/version/c_71e4c1898caa6e32info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/210797https://dx.doi.org/urn:doi:10.1016/j.aca.2019.05.038reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengEuropean Commission https://doi.org/10.13039/501100000780 644956Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 SEV-2017-0706Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 FICTS-1420-27Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 SEV-2015-0522Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 TEC2016-75080-RAgència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2017/SGR-1634open accesshttp://purl.org/coar/access_right/c_abf2Aquest material està protegit per drets d'autor i/o drets afins. Podeu utilitzar aquest material en funció del que permet la legislació de drets d'autor i drets afins d'aplicació al vostre cas. Per a d'altres usos heu d'obtenir permís del(s) titular(s) de drets.https://rightsstatements.org/vocab/InC/1.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2107972026-06-06T12:50:31Z
dc.title.none.fl_str_mv Early sepsis diagnosis via protein and miRNA biomarkers using a novel point-of-care photonic biosensor
title Early sepsis diagnosis via protein and miRNA biomarkers using a novel point-of-care photonic biosensor
spellingShingle Early sepsis diagnosis via protein and miRNA biomarkers using a novel point-of-care photonic biosensor
Fabri Faja, Nuria|||0000-0002-6778-8467
Point-of-care
Label-free multiplexed biosensing
Nanoplasmonics
Sepsis
Protein and miRNA biomarkers
Microarray
title_short Early sepsis diagnosis via protein and miRNA biomarkers using a novel point-of-care photonic biosensor
title_full Early sepsis diagnosis via protein and miRNA biomarkers using a novel point-of-care photonic biosensor
title_fullStr Early sepsis diagnosis via protein and miRNA biomarkers using a novel point-of-care photonic biosensor
title_full_unstemmed Early sepsis diagnosis via protein and miRNA biomarkers using a novel point-of-care photonic biosensor
title_sort Early sepsis diagnosis via protein and miRNA biomarkers using a novel point-of-care photonic biosensor
dc.creator.none.fl_str_mv Fabri Faja, Nuria|||0000-0002-6778-8467
Calvo Lozano, Olalla|||0000-0001-5486-2237
Dey, Priyanka|||0000-0002-7158-4491
Terborg, Roland A.
Estévez, M. Carmen|||0000-0003-3694-7186
Belushkin, Alexander|||0000-0003-1928-2223
Yesilköy, Filiz|||0000-0001-8483-3285
Duempelmann, Luc
Altug, Hatice|||0000-0001-5522-1343
Pruneri, Valerio
Lechuga, Laura M.|||0000-0001-5187-5358
author Fabri Faja, Nuria|||0000-0002-6778-8467
author_facet Fabri Faja, Nuria|||0000-0002-6778-8467
Calvo Lozano, Olalla|||0000-0001-5486-2237
Dey, Priyanka|||0000-0002-7158-4491
Terborg, Roland A.
Estévez, M. Carmen|||0000-0003-3694-7186
Belushkin, Alexander|||0000-0003-1928-2223
Yesilköy, Filiz|||0000-0001-8483-3285
Duempelmann, Luc
Altug, Hatice|||0000-0001-5522-1343
Pruneri, Valerio
Lechuga, Laura M.|||0000-0001-5187-5358
author_role author
author2 Calvo Lozano, Olalla|||0000-0001-5486-2237
Dey, Priyanka|||0000-0002-7158-4491
Terborg, Roland A.
Estévez, M. Carmen|||0000-0003-3694-7186
Belushkin, Alexander|||0000-0003-1928-2223
Yesilköy, Filiz|||0000-0001-8483-3285
Duempelmann, Luc
Altug, Hatice|||0000-0001-5522-1343
Pruneri, Valerio
Lechuga, Laura M.|||0000-0001-5187-5358
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Point-of-care
Label-free multiplexed biosensing
Nanoplasmonics
Sepsis
Protein and miRNA biomarkers
Microarray
topic Point-of-care
Label-free multiplexed biosensing
Nanoplasmonics
Sepsis
Protein and miRNA biomarkers
Microarray
description Sepsis is a condition characterized by a severe stage of blood-infection often leading to tissue damage, organ failure and finally death. Fast diagnosis and identification of the sepsis stage (sepsis, severe sepsis or septic shock) is critical for the patient's evolution and could help in defining the most adequate treatment in order to reduce its mortality. The combined detection of several biomarkers in a timely, specific and simultaneous way could ensure a more accurate diagnosis. We have designed a new optical point-of-care (POC) device based on a phase-sensitive interferometric biosensor with a label-free microarray configuration for potential high-throughput evaluation of specific sepsis biomarkers. The sensor chip, which relies on the use of metallic nanostructures, provides versatility in terms of biofunctionalization, allowing the efficient immobilization of different kind of receptors such as antibodies or oligonucleotides. We have focused on two structurally different types of biomarkers: proteins, including C-reactive protein (CRP) and Interleukin 6 (IL6), and miRNAs, using miRNA-16 as an example. Limits of Detection (LoD) of 18 μg mL, 88 μg mL and 1 μM (6 μg mL) have been respectively obtained for CRP, IL6 and miRNA-16 in individual assays, with high accuracy and reproducibility. The multiplexing capabilities have also been assessed with the simultaneous analysis of both protein biomarkers.
publishDate 2019
dc.date.none.fl_str_mv 2
2019-01-01
2019
2019-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
SMUR
http://purl.org/coar/version/c_71e4c1898caa6e32
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/210797
https://dx.doi.org/urn:doi:10.1016/j.aca.2019.05.038
url https://ddd.uab.cat/record/210797
https://dx.doi.org/urn:doi:10.1016/j.aca.2019.05.038
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv European Commission https://doi.org/10.13039/501100000780 644956
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 SEV-2017-0706
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 FICTS-1420-27
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 SEV-2015-0522
Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 TEC2016-75080-R
Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2017/SGR-1634
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://rightsstatements.org/vocab/InC/1.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://rightsstatements.org/vocab/InC/1.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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