Gene expression profile of epithelial-mesenchymal transition in tumours of patients with nonsmall cell lung cancer. The influence of COPD

Epithelial-mesenchymal transition (EMT) is involved in the pathophysiology of lung cancer (LC) and COPD, and the latter is an important risk factor for LC. We hypothesised that the EMT gene expression profile and signalling cascade may differ in LC patients with COPD from those with no respiratory d...

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Detalles Bibliográficos
Autores: Xia, Yingchen, Zha, Jianhua, Curull Serrano, Víctor, Sánchez-Font, Albert, Guitart, Maria, Rodríguez-Fuster, Alberto, Aguiló Espases, Rafael, Barreiro Portela, Esther
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10230/55604
Acceso en línea:http://hdl.handle.net/10230/55604
http://dx.doi.org/10.1183/23120541.00105-2022
Access Level:acceso abierto
Palabra clave:Pulmons--Càncer
Genètica
Gens
Aparell respiratori--Malalties
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spelling Gene expression profile of epithelial-mesenchymal transition in tumours of patients with nonsmall cell lung cancer. The influence of COPDXia, YingchenZha, JianhuaCurull Serrano, VíctorSánchez-Font, AlbertGuitart, MariaRodríguez-Fuster, AlbertoAguiló Espases, RafaelBarreiro Portela, EstherPulmons--CàncerGenèticaGensAparell respiratori--MalaltiesEpithelial-mesenchymal transition (EMT) is involved in the pathophysiology of lung cancer (LC) and COPD, and the latter is an important risk factor for LC. We hypothesised that the EMT gene expression profile and signalling cascade may differ in LC patients with COPD from those with no respiratory diseases. In lung tumour specimens obtained through video-assisted thoracoscopic surgery from LC (n=20, control group) and LC-COPD patients (n=30), gene expression (quantitative real-time PCR amplification) of EMT markers SMAD3, SMAD4, ZEB2, TWIST1, SNAI1, ICAM1, VIM, CDH2, MMP1 and MMP9 was detected. In lung tumours of LC-COPD compared to LC patients, gene expression of SMAD3, SMAD4, ZEB2 and CDH2 significantly declined, while no significant differences were detected for the other analysed markers. A significant correlation was found between pack-years (smoking burden) and SMAD3 gene expression among LC-COPD patients. LC-COPD patients exhibited mild-to-moderate airway obstruction and a significant reduction in diffusion capacity compared to LC patients. In lung tumour samples of patients with COPD, several markers of EMT expression, namely SMAD3, SMAD4, ZEB2 and CDH2, were differentially expressed suggesting that these markers are likely to play a role in the regulation of EMT in patients with this respiratory disease. Cigarette smoke did not seem to influence the expression of EMT markers in this study. These results have potential clinical implications in the management of patients with LC, particularly in those with underlying respiratory diseases.European Respiratory Society202320232022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/55604http://dx.doi.org/10.1183/23120541.00105-2022reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésThis version is distributed under the terms of the Creative Commons Attribution Non-Commercial Licence 4.0. (http://creativecommons.org/licenses/by/4.0/). For commercial reproduction rights and permissions contact permissions@ersnet.orghttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:10230/556042026-05-29T05:05:01Z
dc.title.none.fl_str_mv Gene expression profile of epithelial-mesenchymal transition in tumours of patients with nonsmall cell lung cancer. The influence of COPD
title Gene expression profile of epithelial-mesenchymal transition in tumours of patients with nonsmall cell lung cancer. The influence of COPD
spellingShingle Gene expression profile of epithelial-mesenchymal transition in tumours of patients with nonsmall cell lung cancer. The influence of COPD
Xia, Yingchen
Pulmons--Càncer
Genètica
Gens
Aparell respiratori--Malalties
title_short Gene expression profile of epithelial-mesenchymal transition in tumours of patients with nonsmall cell lung cancer. The influence of COPD
title_full Gene expression profile of epithelial-mesenchymal transition in tumours of patients with nonsmall cell lung cancer. The influence of COPD
title_fullStr Gene expression profile of epithelial-mesenchymal transition in tumours of patients with nonsmall cell lung cancer. The influence of COPD
title_full_unstemmed Gene expression profile of epithelial-mesenchymal transition in tumours of patients with nonsmall cell lung cancer. The influence of COPD
title_sort Gene expression profile of epithelial-mesenchymal transition in tumours of patients with nonsmall cell lung cancer. The influence of COPD
dc.creator.none.fl_str_mv Xia, Yingchen
Zha, Jianhua
Curull Serrano, Víctor
Sánchez-Font, Albert
Guitart, Maria
Rodríguez-Fuster, Alberto
Aguiló Espases, Rafael
Barreiro Portela, Esther
author Xia, Yingchen
author_facet Xia, Yingchen
Zha, Jianhua
Curull Serrano, Víctor
Sánchez-Font, Albert
Guitart, Maria
Rodríguez-Fuster, Alberto
Aguiló Espases, Rafael
Barreiro Portela, Esther
author_role author
author2 Zha, Jianhua
Curull Serrano, Víctor
Sánchez-Font, Albert
Guitart, Maria
Rodríguez-Fuster, Alberto
Aguiló Espases, Rafael
Barreiro Portela, Esther
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Pulmons--Càncer
Genètica
Gens
Aparell respiratori--Malalties
topic Pulmons--Càncer
Genètica
Gens
Aparell respiratori--Malalties
description Epithelial-mesenchymal transition (EMT) is involved in the pathophysiology of lung cancer (LC) and COPD, and the latter is an important risk factor for LC. We hypothesised that the EMT gene expression profile and signalling cascade may differ in LC patients with COPD from those with no respiratory diseases. In lung tumour specimens obtained through video-assisted thoracoscopic surgery from LC (n=20, control group) and LC-COPD patients (n=30), gene expression (quantitative real-time PCR amplification) of EMT markers SMAD3, SMAD4, ZEB2, TWIST1, SNAI1, ICAM1, VIM, CDH2, MMP1 and MMP9 was detected. In lung tumours of LC-COPD compared to LC patients, gene expression of SMAD3, SMAD4, ZEB2 and CDH2 significantly declined, while no significant differences were detected for the other analysed markers. A significant correlation was found between pack-years (smoking burden) and SMAD3 gene expression among LC-COPD patients. LC-COPD patients exhibited mild-to-moderate airway obstruction and a significant reduction in diffusion capacity compared to LC patients. In lung tumour samples of patients with COPD, several markers of EMT expression, namely SMAD3, SMAD4, ZEB2 and CDH2, were differentially expressed suggesting that these markers are likely to play a role in the regulation of EMT in patients with this respiratory disease. Cigarette smoke did not seem to influence the expression of EMT markers in this study. These results have potential clinical implications in the management of patients with LC, particularly in those with underlying respiratory diseases.
publishDate 2022
dc.date.none.fl_str_mv 2022
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/55604
http://dx.doi.org/10.1183/23120541.00105-2022
url http://hdl.handle.net/10230/55604
http://dx.doi.org/10.1183/23120541.00105-2022
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv European Respiratory Society
publisher.none.fl_str_mv European Respiratory Society
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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