Patch method for culture of primary hippocampal neurons

Culture of primary neurons, and especially hippocampal neurons, is important for understanding cellular mechanisms in neurobiology. Actually, this is achieved by using culture dish or glass slide with surface coated proteins. Here, we proposed a patch method for culture of primary neurons on a monol...

Descripción completa

Detalles Bibliográficos
Autores: Tang, Y., Ulloa Severino, F.P., Iseppon, F., Torre, V., Chen, Y.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/414485
Acceso en línea:http://hdl.handle.net/10261/414485
http://www.scopus.com/inward/record.url?eid=2-s2.0-85010204755&partnerID=MN8TOARS
Access Level:acceso abierto
Palabra clave:Calcium imaging
Patch
Hippocampal neuron
http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
id ES_57a72d59af0d8fc3507acb9933e2e721
oai_identifier_str oai:digital.csic.es:10261/414485
network_acronym_str ES
network_name_str España
repository_id_str
spelling Patch method for culture of primary hippocampal neuronsTang, Y.Ulloa Severino, F.P.Iseppon, F.Torre, V.Chen, Y.Calcium imagingPatchHippocampal neuronCalcium imaginghttp://metadata.un.org/sdg/3Ensure healthy lives and promote well-being for all at all agesCulture of primary neurons, and especially hippocampal neurons, is important for understanding cellular mechanisms in neurobiology. Actually, this is achieved by using culture dish or glass slide with surface coated proteins. Here, we proposed a patch method for culture of primary neurons on a monolayer of gelatin nanofibers electrospun and crosslinked on a honeycomb microframe of poly (ethylene glycol) diacrylate (PEGDA). This method allows us to minimize exogenous material contact of cells and largely increase the exposure area of cells to the culture medium. We found that neurons, and especially astrocytes, have a more in vivo like morphology comparing to that on culture dish or on glass slide. We also found that neurons were preferentially located in the suspended areas of the monolayer nanofibers. Finally, calcium imaging revealed that primary neurons have a higher degree of neural activity on the patch than on glass. These results suggest that crosslinked and monolayer gelatin nanofibers closely mimic the extracellular matrix structure and allow more effective culture of primary neurons than conventional methods, thus facilitating advanced studies of neural functions as well as cell-based assays.This work was supported by the European Commission under contract No.604263 (Neuroscaffolds) and Agence de Recherche Nationale under contract No ANR-13-NANO-0011-01 (Pillarcell) and ANR-12-RPIB-0015 (CardiacPatch).Peer reviewedElsevierEuropean CommissionAgence Nationale de la Recherche (France)#NODATA##NODATA##NODATA##NODATA##NODATA#202620262017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/414485http://www.scopus.com/inward/record.url?eid=2-s2.0-85010204755&partnerID=MN8TOARSreponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésMicroelectronic EngineeringMicroelectronic Engineeringhttps://doi.org/10.1016/j.mee.2017.01.012Noinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/4144852026-05-22T06:33:51Z
dc.title.none.fl_str_mv Patch method for culture of primary hippocampal neurons
title Patch method for culture of primary hippocampal neurons
spellingShingle Patch method for culture of primary hippocampal neurons
Tang, Y.
Calcium imaging
Patch
Hippocampal neuron
Calcium imaging
http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
title_short Patch method for culture of primary hippocampal neurons
title_full Patch method for culture of primary hippocampal neurons
title_fullStr Patch method for culture of primary hippocampal neurons
title_full_unstemmed Patch method for culture of primary hippocampal neurons
title_sort Patch method for culture of primary hippocampal neurons
dc.creator.none.fl_str_mv Tang, Y.
Ulloa Severino, F.P.
Iseppon, F.
Torre, V.
Chen, Y.
author Tang, Y.
author_facet Tang, Y.
Ulloa Severino, F.P.
Iseppon, F.
Torre, V.
Chen, Y.
author_role author
author2 Ulloa Severino, F.P.
Iseppon, F.
Torre, V.
Chen, Y.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv European Commission
Agence Nationale de la Recherche (France)
#NODATA#
#NODATA#
#NODATA#
#NODATA#
#NODATA#
dc.subject.none.fl_str_mv Calcium imaging
Patch
Hippocampal neuron
Calcium imaging
http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
topic Calcium imaging
Patch
Hippocampal neuron
Calcium imaging
http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
description Culture of primary neurons, and especially hippocampal neurons, is important for understanding cellular mechanisms in neurobiology. Actually, this is achieved by using culture dish or glass slide with surface coated proteins. Here, we proposed a patch method for culture of primary neurons on a monolayer of gelatin nanofibers electrospun and crosslinked on a honeycomb microframe of poly (ethylene glycol) diacrylate (PEGDA). This method allows us to minimize exogenous material contact of cells and largely increase the exposure area of cells to the culture medium. We found that neurons, and especially astrocytes, have a more in vivo like morphology comparing to that on culture dish or on glass slide. We also found that neurons were preferentially located in the suspended areas of the monolayer nanofibers. Finally, calcium imaging revealed that primary neurons have a higher degree of neural activity on the patch than on glass. These results suggest that crosslinked and monolayer gelatin nanofibers closely mimic the extracellular matrix structure and allow more effective culture of primary neurons than conventional methods, thus facilitating advanced studies of neural functions as well as cell-based assays.
publishDate 2017
dc.date.none.fl_str_mv 2017
2026
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/414485
http://www.scopus.com/inward/record.url?eid=2-s2.0-85010204755&partnerID=MN8TOARS
url http://hdl.handle.net/10261/414485
http://www.scopus.com/inward/record.url?eid=2-s2.0-85010204755&partnerID=MN8TOARS
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Microelectronic Engineering
Microelectronic Engineering
https://doi.org/10.1016/j.mee.2017.01.012
No
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869408468160479232
score 15.812455

Similares en LA Referencia