Dynamic karyotype evolution and unique sex determination systems in Leptidea wood white butterflies

Background. Chromosomal rearrangements have the potential to limit the rate and pattern of gene flow within and between species and thus play a direct role in promoting and maintaining speciation. Wood white butterflies of the genus Leptidea are excellent models to study the role of chromosome rearr...

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Autores: Šíchová, Jindra, Voleníková, Anna, Dincă, Vlad, Nguyen, Petr, Vila, Roger, Sahara, Ken, Marec, František
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10230/25418
Acceso en línea:http://hdl.handle.net/10230/25418
http://dx.doi.org/10.1186/s12862-015-0375-4
Access Level:acceso abierto
Palabra clave:Cromosomes sexuals
Lepidoptera
Wood white butterflies
Karyotype variability
Fluorescence in situ hybridization Chromosome fusion and fission
Multiple sex chromosomes
Speciation
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spelling Dynamic karyotype evolution and unique sex determination systems in Leptidea wood white butterfliesŠíchová, JindraVoleníková, AnnaDincă, VladNguyen, PetrVila, RogerSahara, KenMarec, FrantišekCromosomes sexualsLepidopteraWood white butterfliesKaryotype variabilityFluorescence in situ hybridization Chromosome fusion and fissionMultiple sex chromosomesSpeciationBackground. Chromosomal rearrangements have the potential to limit the rate and pattern of gene flow within and between species and thus play a direct role in promoting and maintaining speciation. Wood white butterflies of the genus Leptidea are excellent models to study the role of chromosome rearrangements in speciation because they show karyotype variability not only among but also within species. In this work, we investigated genome architecture of three cryptic Leptidea species (L. juvernica, L. sinapis and L. reali) by standard and molecular cytogenetic techniques in order to reveal causes of the karyotype variability./nResults. Chromosome numbers ranged from 2n = 85 to 91 in L. juvernica and 2n = 69 to 73 in L. sinapis (both from Czech populations) to 2n = 51 to 55 in L. reali (Spanish population). We observed significant differences in chromosome numbers and localization of cytogenetic markers (rDNA and H3 histone genes) within the offspring of individual females. Using FISH with the (TTAGG)n telomeric probe we also documented the presence of multiple chromosome fusions and/or fissions and other complex rearrangements. Thus, the intraspecific karyotype variability is likely due to irregular chromosome segregation of multivalent meiotic configurations. The analysis of female meiotic chromosomes by GISH and CGH revealed multiple sex chromosomes: W1W2W3Z1Z2Z3Z4 in L. juvernica, W1W2W3Z1Z2Z3 in L. sinapis and W1W2W3W4Z1Z2Z3Z4 in L. reali./nConclusions. Our results suggest a dynamic karyotype evolution and point to the role of chromosomal rearrangements in the speciation of Leptidea butterflies. Moreover, our study revealed a curious sex determination system with 3–4 W and 3–4 Z chromosomes, which is unique in the Lepidoptera and which could also have played a role in the speciation process of the three Leptidea species.This research was funded by Grant 14-22765S of the Czech Science Foundation, Grant IAA600960925 of the Grant Agency of The Czech Academy of Sciences, Grant 063/2012/P of the Grant Agency of the University of South Bohemia and Grant CGL2013-48277-P from the Spanish Ministerio de Economía y Competitividad. JŠ and AV were supported by Grant 052/2013/P of the Grant Agency of the University of South Bohemia, VD by a Marie Curie International Outgoing Fellowship within the 7th European Community Framework Programme (project no. 625997), PN by Grant 14-35819P of the Czech Science Foundation and KS by JSPS 23380030 grant and JSPS Excellent Young Researchers Overseas Visit Program (21–7147)BioMed Central201520152015info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/25418http://dx.doi.org/10.1186/s12862-015-0375-4reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésBMC Evolutionary Biology. 2015;15:89info:eu-repo/grantAgreement/EC/FP7/625997© 2015 Šíchová et al.; licensee BioMed Central. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.http://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessoai:recercat.cat:10230/254182026-05-29T05:05:01Z
dc.title.none.fl_str_mv Dynamic karyotype evolution and unique sex determination systems in Leptidea wood white butterflies
title Dynamic karyotype evolution and unique sex determination systems in Leptidea wood white butterflies
spellingShingle Dynamic karyotype evolution and unique sex determination systems in Leptidea wood white butterflies
Šíchová, Jindra
Cromosomes sexuals
Lepidoptera
Wood white butterflies
Karyotype variability
Fluorescence in situ hybridization Chromosome fusion and fission
Multiple sex chromosomes
Speciation
title_short Dynamic karyotype evolution and unique sex determination systems in Leptidea wood white butterflies
title_full Dynamic karyotype evolution and unique sex determination systems in Leptidea wood white butterflies
title_fullStr Dynamic karyotype evolution and unique sex determination systems in Leptidea wood white butterflies
title_full_unstemmed Dynamic karyotype evolution and unique sex determination systems in Leptidea wood white butterflies
title_sort Dynamic karyotype evolution and unique sex determination systems in Leptidea wood white butterflies
dc.creator.none.fl_str_mv Šíchová, Jindra
Voleníková, Anna
Dincă, Vlad
Nguyen, Petr
Vila, Roger
Sahara, Ken
Marec, František
author Šíchová, Jindra
author_facet Šíchová, Jindra
Voleníková, Anna
Dincă, Vlad
Nguyen, Petr
Vila, Roger
Sahara, Ken
Marec, František
author_role author
author2 Voleníková, Anna
Dincă, Vlad
Nguyen, Petr
Vila, Roger
Sahara, Ken
Marec, František
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Cromosomes sexuals
Lepidoptera
Wood white butterflies
Karyotype variability
Fluorescence in situ hybridization Chromosome fusion and fission
Multiple sex chromosomes
Speciation
topic Cromosomes sexuals
Lepidoptera
Wood white butterflies
Karyotype variability
Fluorescence in situ hybridization Chromosome fusion and fission
Multiple sex chromosomes
Speciation
description Background. Chromosomal rearrangements have the potential to limit the rate and pattern of gene flow within and between species and thus play a direct role in promoting and maintaining speciation. Wood white butterflies of the genus Leptidea are excellent models to study the role of chromosome rearrangements in speciation because they show karyotype variability not only among but also within species. In this work, we investigated genome architecture of three cryptic Leptidea species (L. juvernica, L. sinapis and L. reali) by standard and molecular cytogenetic techniques in order to reveal causes of the karyotype variability./nResults. Chromosome numbers ranged from 2n = 85 to 91 in L. juvernica and 2n = 69 to 73 in L. sinapis (both from Czech populations) to 2n = 51 to 55 in L. reali (Spanish population). We observed significant differences in chromosome numbers and localization of cytogenetic markers (rDNA and H3 histone genes) within the offspring of individual females. Using FISH with the (TTAGG)n telomeric probe we also documented the presence of multiple chromosome fusions and/or fissions and other complex rearrangements. Thus, the intraspecific karyotype variability is likely due to irregular chromosome segregation of multivalent meiotic configurations. The analysis of female meiotic chromosomes by GISH and CGH revealed multiple sex chromosomes: W1W2W3Z1Z2Z3Z4 in L. juvernica, W1W2W3Z1Z2Z3 in L. sinapis and W1W2W3W4Z1Z2Z3Z4 in L. reali./nConclusions. Our results suggest a dynamic karyotype evolution and point to the role of chromosomal rearrangements in the speciation of Leptidea butterflies. Moreover, our study revealed a curious sex determination system with 3–4 W and 3–4 Z chromosomes, which is unique in the Lepidoptera and which could also have played a role in the speciation process of the three Leptidea species.
publishDate 2015
dc.date.none.fl_str_mv 2015
2015
2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/25418
http://dx.doi.org/10.1186/s12862-015-0375-4
url http://hdl.handle.net/10230/25418
http://dx.doi.org/10.1186/s12862-015-0375-4
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv BMC Evolutionary Biology. 2015;15:89
info:eu-repo/grantAgreement/EC/FP7/625997
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
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repository.mail.fl_str_mv
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