Multi-reaction kinetic modeling for the peroxidase-aldolase cascade synthesis of a D-fagomine precursor

The feasibility of a peroxidase-aldolase cascade reaction for the synthesis of therapeutically-valuable iminocyclitols is discussed herein. A two-enzyme system consisting of chloroperoxidase (CPO) and D-fructose-6-phosphate aldolase (FSA) was evaluated for the synthesis of a D-fagomine precursor (pr...

Descripción completa

Detalles Bibliográficos
Autores: Masdeu, Gerard|||0000-0002-4726-1545, Findrik Blažević, Zvjezdana|||0000-0002-5312-8951, Kralj, Slavko, Makovec, Darko|||0000-0002-0190-6758, López Santín, Josep|||0000-0002-6039-8044, Álvaro, Gregorio|||0000-0002-2924-8902
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:250666
Acceso en línea:https://ddd.uab.cat/record/250666
https://dx.doi.org/urn:doi:10.1016/j.ces.2021.116602
Access Level:acceso abierto
Palabra clave:Enzymatic reaction cascade
Peroxidase
Aldolase
D-fagomine
Kinetic modeling
Descripción
Sumario:The feasibility of a peroxidase-aldolase cascade reaction for the synthesis of therapeutically-valuable iminocyclitols is discussed herein. A two-enzyme system consisting of chloroperoxidase (CPO) and D-fructose-6-phosphate aldolase (FSA) was evaluated for the synthesis of a D-fagomine precursor (preFagomine) from a N-Cbz-3-aminopropanol. An in-depth, systematic, step-by-step kinetic modeling of seven reactions and two inactivation decays was proposed to elucidate the reaction mechanism, prepare suitable stabilized biocatalysts, and find the optimal conditions for its application. The model described accurately the data and predicted the outcome at different experimental conditions. The inactivation of FSA caused by CPO was identified as the main bottleneck in the reaction. A two-step reaction approach and the use of immobilized enzymes on magnetic nanoparticle clusters and functionalized agarose carriers increased the stability of FSA, with an 1839-fold higher preFagomine formation per mol of enzyme in comparison to a one-pot reaction using soluble enzymes.