IFNAR(-/-) Mice Constitute a Suitable Animal Model for Epizootic Hemorrhagic Disease Virus Study and Vaccine Evaluation

[EN] Epizootic hemorrhagic disease (EHD), caused by Epizootic hemorrhagic disease virus (EHDV), is an emerging and severe livestock disease. Recent incursion and distribution of EHDV in Europe have outlined the emerging character of EHD. Despite its worldwide impact, numerous knowledge gaps exist. A...

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Autores: Jiménez Cabello, Luis, Utrilla Trigo, Sergio, Benavides Silván, Julio, Anguita, Juan, Calvo Pinilla, Eva, Ortego, Javier
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Recursos:Universidad de León
Repositorio:BULERIA. Repositorio Institucional de la Universidad de León
OAI Identifier:oai:buleria.unileon.es:10612/26147
Acesso em linha:https://www.ijbs.com/v20p3076.htm
https://hdl.handle.net/10612/26147
Access Level:acceso abierto
Palavra-chave:Sanidad animal
Veterinaria
Epizootic hemorrhagic disease virus
IFNAR(-/-) mouse
Vaccine
Bluetongue virus
Viral coinfection
3109.03 Inmunología
3109.07 Patología
3109.11 Virología
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repository_id_str
spelling IFNAR(-/-) Mice Constitute a Suitable Animal Model for Epizootic Hemorrhagic Disease Virus Study and Vaccine EvaluationJiménez Cabello, LuisUtrilla Trigo, SergioBenavides Silván, JulioAnguita, JuanCalvo Pinilla, EvaOrtego, JavierSanidad animalVeterinariaEpizootic hemorrhagic disease virusIFNAR(-/-) mouseVaccineBluetongue virusViral coinfection3109.03 Inmunología3109.07 Patología3109.11 Virología[EN] Epizootic hemorrhagic disease (EHD), caused by Epizootic hemorrhagic disease virus (EHDV), is an emerging and severe livestock disease. Recent incursion and distribution of EHDV in Europe have outlined the emerging character of EHD. Despite its worldwide impact, numerous knowledge gaps exist. A range of inconveniences restricts utilization of natural hosts of EHDV. Here, we show that adult mice deficient in type I IFN receptor (IFNAR(-/-)) are highly susceptible to EHDV-6 and EHDV-8 infection when the virus is administered subcutaneously. Disease was characterized by ruffled hair, reluctance to move, dehydration and conjunctivitis, with viraemia detected from day 5 post-infection. A deeper characterization of EHDV-8 infection showed viral replication in the lung, liver, spleen, kidney, testis and ovaries. Importantly, increased expression levels of pro-inflammatory cytokines IL-1β, IL-6 and CXCL2 were observed in spleen after EHDV-8 infection. Furthermore, IFNAR(-/-) adult mice immunized with a EHDV-8 inactivated vaccine elicited neutralizing antibodies specific of EHDV-8 and full protection against challenge with a lethal dose of this virus. This study also explores the possibilities of this animal model for study of BTV and EHDV coinfection. In summary, the IFNAR(-/-) mouse model faithfully recapitulates EHD and can be applied for vaccine testing, which can facilitate progress in addressing the animal health challenge posed by this virusSIIvyspring International PublisherSanidad AnimalFacultad de Veterinaria2024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://www.ijbs.com/v20p3076.htmhttps://hdl.handle.net/10612/26147reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Universidad de LeónInglésinfo:eu-repo/grantAgreement/AEI/Programa Estatal de I+D+i Orientada a los Retos de la Sociedad/PID2020-112992RR-I00http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:buleria.unileon.es:10612/261472026-06-24T12:43:27Z
dc.title.none.fl_str_mv IFNAR(-/-) Mice Constitute a Suitable Animal Model for Epizootic Hemorrhagic Disease Virus Study and Vaccine Evaluation
title IFNAR(-/-) Mice Constitute a Suitable Animal Model for Epizootic Hemorrhagic Disease Virus Study and Vaccine Evaluation
spellingShingle IFNAR(-/-) Mice Constitute a Suitable Animal Model for Epizootic Hemorrhagic Disease Virus Study and Vaccine Evaluation
Jiménez Cabello, Luis
Sanidad animal
Veterinaria
Epizootic hemorrhagic disease virus
IFNAR(-/-) mouse
Vaccine
Bluetongue virus
Viral coinfection
3109.03 Inmunología
3109.07 Patología
3109.11 Virología
title_short IFNAR(-/-) Mice Constitute a Suitable Animal Model for Epizootic Hemorrhagic Disease Virus Study and Vaccine Evaluation
title_full IFNAR(-/-) Mice Constitute a Suitable Animal Model for Epizootic Hemorrhagic Disease Virus Study and Vaccine Evaluation
title_fullStr IFNAR(-/-) Mice Constitute a Suitable Animal Model for Epizootic Hemorrhagic Disease Virus Study and Vaccine Evaluation
title_full_unstemmed IFNAR(-/-) Mice Constitute a Suitable Animal Model for Epizootic Hemorrhagic Disease Virus Study and Vaccine Evaluation
title_sort IFNAR(-/-) Mice Constitute a Suitable Animal Model for Epizootic Hemorrhagic Disease Virus Study and Vaccine Evaluation
dc.creator.none.fl_str_mv Jiménez Cabello, Luis
Utrilla Trigo, Sergio
Benavides Silván, Julio
Anguita, Juan
Calvo Pinilla, Eva
Ortego, Javier
author Jiménez Cabello, Luis
author_facet Jiménez Cabello, Luis
Utrilla Trigo, Sergio
Benavides Silván, Julio
Anguita, Juan
Calvo Pinilla, Eva
Ortego, Javier
author_role author
author2 Utrilla Trigo, Sergio
Benavides Silván, Julio
Anguita, Juan
Calvo Pinilla, Eva
Ortego, Javier
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Sanidad Animal
Facultad de Veterinaria
dc.subject.none.fl_str_mv Sanidad animal
Veterinaria
Epizootic hemorrhagic disease virus
IFNAR(-/-) mouse
Vaccine
Bluetongue virus
Viral coinfection
3109.03 Inmunología
3109.07 Patología
3109.11 Virología
topic Sanidad animal
Veterinaria
Epizootic hemorrhagic disease virus
IFNAR(-/-) mouse
Vaccine
Bluetongue virus
Viral coinfection
3109.03 Inmunología
3109.07 Patología
3109.11 Virología
description [EN] Epizootic hemorrhagic disease (EHD), caused by Epizootic hemorrhagic disease virus (EHDV), is an emerging and severe livestock disease. Recent incursion and distribution of EHDV in Europe have outlined the emerging character of EHD. Despite its worldwide impact, numerous knowledge gaps exist. A range of inconveniences restricts utilization of natural hosts of EHDV. Here, we show that adult mice deficient in type I IFN receptor (IFNAR(-/-)) are highly susceptible to EHDV-6 and EHDV-8 infection when the virus is administered subcutaneously. Disease was characterized by ruffled hair, reluctance to move, dehydration and conjunctivitis, with viraemia detected from day 5 post-infection. A deeper characterization of EHDV-8 infection showed viral replication in the lung, liver, spleen, kidney, testis and ovaries. Importantly, increased expression levels of pro-inflammatory cytokines IL-1β, IL-6 and CXCL2 were observed in spleen after EHDV-8 infection. Furthermore, IFNAR(-/-) adult mice immunized with a EHDV-8 inactivated vaccine elicited neutralizing antibodies specific of EHDV-8 and full protection against challenge with a lethal dose of this virus. This study also explores the possibilities of this animal model for study of BTV and EHDV coinfection. In summary, the IFNAR(-/-) mouse model faithfully recapitulates EHD and can be applied for vaccine testing, which can facilitate progress in addressing the animal health challenge posed by this virus
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.ijbs.com/v20p3076.htm
https://hdl.handle.net/10612/26147
url https://www.ijbs.com/v20p3076.htm
https://hdl.handle.net/10612/26147
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/AEI/Programa Estatal de I+D+i Orientada a los Retos de la Sociedad/PID2020-112992RR-I00
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Ivyspring International Publisher
publisher.none.fl_str_mv Ivyspring International Publisher
dc.source.none.fl_str_mv reponame:BULERIA. Repositorio Institucional de la Universidad de León
instname:Universidad de León
instname_str Universidad de León
reponame_str BULERIA. Repositorio Institucional de la Universidad de León
collection BULERIA. Repositorio Institucional de la Universidad de León
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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