Biotic factor does not limit operational pH in packed-bed bioreactor for ferrous iron biooxidation

Ferrous ion biooxidation is a process with many promising industrial applications: mainly regeneration of ferric ion as an oxidizing reagent in bioleaching processes and depuration of acid mine drainage. The flooded packedbed bioreactor (FPB) is the design that leads to the highest biooxidation rate...

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Autores: Mazuelos Rojas, Alfonso, Moreno, José María, Carranza Mora, Francisco, Palomino, Carmen, Torres Rueda, Antonio Ildefonso, Villalobo Polo, Eduardo
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2012
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/156506
Acceso en línea:https://hdl.handle.net/11441/156506
https://doi.org/10.1007/s10295-012-1187-9
Access Level:acceso abierto
Palabra clave:Biofilms
Ferrous ion biooxidation
Immobilised cells
Mesophiles
Mixed inocula
Packed-bed bioreactors
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spelling Biotic factor does not limit operational pH in packed-bed bioreactor for ferrous iron biooxidationMazuelos Rojas, AlfonsoMoreno, José MaríaCarranza Mora, FranciscoPalomino, CarmenTorres Rueda, Antonio IldefonsoVillalobo Polo, EduardoBiofilmsFerrous ion biooxidationImmobilised cellsMesophilesMixed inoculaPacked-bed bioreactorsFerrous ion biooxidation is a process with many promising industrial applications: mainly regeneration of ferric ion as an oxidizing reagent in bioleaching processes and depuration of acid mine drainage. The flooded packedbed bioreactor (FPB) is the design that leads to the highest biooxidation rate. In this bioreactor, biomass is immobilized in a biofilm that consists of an inorganic matrix, formed by precipitated ferric compounds, in the pores of which cells are attached. This biofilm covers the surface of particles (siliceous stone) that form the bed. The chemical stability of this inorganic matrix defines the widest possible pH range in FPBs. At pH below 1, ferric matrix is dissolved and cells are washed out. At pH higher than 2, ferric ion precipitates massively, greatly hindering mass transfer to cells. Thus, among other parameters, pH is recognised as a key factor for operational control in FPBs. This paper aims to explain the effect of pH on FPB operation, with an emphasis on microbial population behaviour. FPBs seeded with mixed inocula were assayed in the pH range from 2.3 to 0.8 and the microbial population was characterised. The microbial consortium in the bioreactor is modified by pH; at pH above 1.3 Acidithiobacillus ferrooxidans is the dominant microorganism, whereas at pH below 1.3 Leptospirillum ferrooxidans dominates. Inoculum can be adapted to acidity during continuous operation by progressively decreasing the pH of the inlet solution. Thus, in the pH range from 2.3 to 1, the biotic factor does not compromise the bioreactor performance.Ministerio de Ciencia e Innovación BFU2009-10393Oxford University PressIngeniería QuímicaMicrobiologíaMinisterio de Ciencia e Innovación (MICIN). España2012info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/156506https://doi.org/10.1007/s10295-012-1187-9reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésJournal of Industrial Microbiology and Biotechnology, 39 (12), 1851-1858.BFU2009-10393https://doi.org/10.1007/s10295-012-1187-9info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1565062026-06-17T12:51:07Z
dc.title.none.fl_str_mv Biotic factor does not limit operational pH in packed-bed bioreactor for ferrous iron biooxidation
title Biotic factor does not limit operational pH in packed-bed bioreactor for ferrous iron biooxidation
spellingShingle Biotic factor does not limit operational pH in packed-bed bioreactor for ferrous iron biooxidation
Mazuelos Rojas, Alfonso
Biofilms
Ferrous ion biooxidation
Immobilised cells
Mesophiles
Mixed inocula
Packed-bed bioreactors
title_short Biotic factor does not limit operational pH in packed-bed bioreactor for ferrous iron biooxidation
title_full Biotic factor does not limit operational pH in packed-bed bioreactor for ferrous iron biooxidation
title_fullStr Biotic factor does not limit operational pH in packed-bed bioreactor for ferrous iron biooxidation
title_full_unstemmed Biotic factor does not limit operational pH in packed-bed bioreactor for ferrous iron biooxidation
title_sort Biotic factor does not limit operational pH in packed-bed bioreactor for ferrous iron biooxidation
dc.creator.none.fl_str_mv Mazuelos Rojas, Alfonso
Moreno, José María
Carranza Mora, Francisco
Palomino, Carmen
Torres Rueda, Antonio Ildefonso
Villalobo Polo, Eduardo
author Mazuelos Rojas, Alfonso
author_facet Mazuelos Rojas, Alfonso
Moreno, José María
Carranza Mora, Francisco
Palomino, Carmen
Torres Rueda, Antonio Ildefonso
Villalobo Polo, Eduardo
author_role author
author2 Moreno, José María
Carranza Mora, Francisco
Palomino, Carmen
Torres Rueda, Antonio Ildefonso
Villalobo Polo, Eduardo
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Ingeniería Química
Microbiología
Ministerio de Ciencia e Innovación (MICIN). España
dc.subject.none.fl_str_mv Biofilms
Ferrous ion biooxidation
Immobilised cells
Mesophiles
Mixed inocula
Packed-bed bioreactors
topic Biofilms
Ferrous ion biooxidation
Immobilised cells
Mesophiles
Mixed inocula
Packed-bed bioreactors
description Ferrous ion biooxidation is a process with many promising industrial applications: mainly regeneration of ferric ion as an oxidizing reagent in bioleaching processes and depuration of acid mine drainage. The flooded packedbed bioreactor (FPB) is the design that leads to the highest biooxidation rate. In this bioreactor, biomass is immobilized in a biofilm that consists of an inorganic matrix, formed by precipitated ferric compounds, in the pores of which cells are attached. This biofilm covers the surface of particles (siliceous stone) that form the bed. The chemical stability of this inorganic matrix defines the widest possible pH range in FPBs. At pH below 1, ferric matrix is dissolved and cells are washed out. At pH higher than 2, ferric ion precipitates massively, greatly hindering mass transfer to cells. Thus, among other parameters, pH is recognised as a key factor for operational control in FPBs. This paper aims to explain the effect of pH on FPB operation, with an emphasis on microbial population behaviour. FPBs seeded with mixed inocula were assayed in the pH range from 2.3 to 0.8 and the microbial population was characterised. The microbial consortium in the bioreactor is modified by pH; at pH above 1.3 Acidithiobacillus ferrooxidans is the dominant microorganism, whereas at pH below 1.3 Leptospirillum ferrooxidans dominates. Inoculum can be adapted to acidity during continuous operation by progressively decreasing the pH of the inlet solution. Thus, in the pH range from 2.3 to 1, the biotic factor does not compromise the bioreactor performance.
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/156506
https://doi.org/10.1007/s10295-012-1187-9
url https://hdl.handle.net/11441/156506
https://doi.org/10.1007/s10295-012-1187-9
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Journal of Industrial Microbiology and Biotechnology, 39 (12), 1851-1858.
BFU2009-10393
https://doi.org/10.1007/s10295-012-1187-9
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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