Metabolic profiling of (poly)phenolic compounds in mouse urine following consumption of hull-less and purple-grain barley

The present study attempted for the first time to investigate the metabolic fate of (poly)phenolic compounds provided by a hull-less and purple grain barley genotype biofortified in anthocyanins. Balb/c mice were supplemented either with standard purified diet (SD) or whole-grain barley supplemented...

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Autores: Cortijo-Alfonso, Maria Engracia, Yuste, Silvia, Friero, Iván, Martínez Subirà, Mariona, Moralejo Vidal, Mª Angeles, Piñol Felis, Carme, Rubió Piqué, Laura, Macià i Puig, Ma Alba
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Universitat de Lleida (UdL)
Repositorio:Repositori Obert UdL
OAI Identifier:oai:repositori.udl.cat:10459.1/466849
Acceso en línea:https://doi.org/10.1039/d4fo01275e
https://hdl.handle.net/10459.1/466849
Access Level:acceso abierto
Palabra clave:Animals
Anthocyanins
Flavonoids
Hordeum
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spelling Metabolic profiling of (poly)phenolic compounds in mouse urine following consumption of hull-less and purple-grain barleyCortijo-Alfonso, Maria EngraciaYuste, SilviaFriero, IvánMartínez Subirà, MarionaMoralejo Vidal, Mª AngelesPiñol Felis, CarmeRubió Piqué, LauraMacià i Puig, Ma AlbaAnimalsAnthocyaninsFlavonoidsHordeumThe present study attempted for the first time to investigate the metabolic fate of (poly)phenolic compounds provided by a hull-less and purple grain barley genotype biofortified in anthocyanins. Balb/c mice were supplemented either with standard purified diet (SD) or whole-grain barley supplemented diet (WGB) for six weeks. Subsequently, (poly)phenolic metabolites were determined in urine samples by UPLC-MS/MS, and the principal metabolic pathways were elucidated. Thirty-nine (poly)phenolics compounds were identified in WGB which were distributed between the free (58%) and bound (42%) fractions, encompassing anthocyanins, phenolic acids, flavan-3-ols and flavones. Upon WGB intake, forty-two (poly)phenolic metabolites were identified, predominantly comprising phase-II sulphate, glucuronide, and/or methylated conjugates, along with colonic catabolites. Noteworthy metabolites included peonidin-3-O-glucuronide, peonidin-3-O-6′′-O-malonylglucoside, and peonidin-3-O-glucoside among anthocyanins; hydroxyphenylpropanoic acid-O-sulphate among phenolic acids; and 5-(3′,4′-dihydroxyphenyl)-γ-valerolactone-O-sulphate among flavan-3-ols. Metabolites like phenylpropionic, phenylacetic, hydroxybenzoic, and hippuric acids were found in both WGB and SD groups, with higher levels after barley consumption, indicating both endogenous and polyphenolic metabolism origins. Overall, this study offers valuable insights into the metabolism of (poly)phenols in purple barley, setting the stage for future investigations into the health benefits linked to the consumption of purple grain barley.This study was supported by the Spanish Ministry of Industry, Economy and Competitiveness, the Agencia Estatal de Investigaci\u00F3n (AEI) and the European Regional Development Fund (ERDF) through the PID2020-113009RB-100 INNOBAR project and by the Generalitat de Catalunya through the Mar\u00EDa Engracia Cortijo predoctoral FI SDUR grant. Silvia Yuste has a Margarita Salas postdoctoral grant funded by the European Union \u2013 NextGenerationEU through the Ministerio de Universidades and the Universitat de Lleida. Iv\u00E1n Friero was supported by a pre-doctoral fellowship (PRE2021-100591). The authors are grateful to SCT-Estabulari of the Universitat de Lleida where the animal experiment was carried out. We would like to acknowledge Dr Mar\u00EDa-Paz Romero for her valuable contribution and supervision throughout this work.Royal Society of Chemistry2024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://doi.org/10.1039/d4fo01275ehttps://hdl.handle.net/10459.1/466849reponame:Repositori Obert UdL instname:Universitat de Lleida (UdL)Inglésinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113009RB-I00Reproducció del document publicat a https://doi.org/10.1039/d4fo01275eFood and Function, 2024, vol.15, núm. 16, p. 8300-8309https://doi.org/10.34810/data1387cc-by-nc (c) Cortijo-Alfonso et al., 2024Attribution-NonCommercial 4.0 Internationalinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc/4.0/oai:repositori.udl.cat:10459.1/4668492026-06-24T12:42:17Z
dc.title.none.fl_str_mv Metabolic profiling of (poly)phenolic compounds in mouse urine following consumption of hull-less and purple-grain barley
title Metabolic profiling of (poly)phenolic compounds in mouse urine following consumption of hull-less and purple-grain barley
spellingShingle Metabolic profiling of (poly)phenolic compounds in mouse urine following consumption of hull-less and purple-grain barley
Cortijo-Alfonso, Maria Engracia
Animals
Anthocyanins
Flavonoids
Hordeum
title_short Metabolic profiling of (poly)phenolic compounds in mouse urine following consumption of hull-less and purple-grain barley
title_full Metabolic profiling of (poly)phenolic compounds in mouse urine following consumption of hull-less and purple-grain barley
title_fullStr Metabolic profiling of (poly)phenolic compounds in mouse urine following consumption of hull-less and purple-grain barley
title_full_unstemmed Metabolic profiling of (poly)phenolic compounds in mouse urine following consumption of hull-less and purple-grain barley
title_sort Metabolic profiling of (poly)phenolic compounds in mouse urine following consumption of hull-less and purple-grain barley
dc.creator.none.fl_str_mv Cortijo-Alfonso, Maria Engracia
Yuste, Silvia
Friero, Iván
Martínez Subirà, Mariona
Moralejo Vidal, Mª Angeles
Piñol Felis, Carme
Rubió Piqué, Laura
Macià i Puig, Ma Alba
author Cortijo-Alfonso, Maria Engracia
author_facet Cortijo-Alfonso, Maria Engracia
Yuste, Silvia
Friero, Iván
Martínez Subirà, Mariona
Moralejo Vidal, Mª Angeles
Piñol Felis, Carme
Rubió Piqué, Laura
Macià i Puig, Ma Alba
author_role author
author2 Yuste, Silvia
Friero, Iván
Martínez Subirà, Mariona
Moralejo Vidal, Mª Angeles
Piñol Felis, Carme
Rubió Piqué, Laura
Macià i Puig, Ma Alba
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Animals
Anthocyanins
Flavonoids
Hordeum
topic Animals
Anthocyanins
Flavonoids
Hordeum
description The present study attempted for the first time to investigate the metabolic fate of (poly)phenolic compounds provided by a hull-less and purple grain barley genotype biofortified in anthocyanins. Balb/c mice were supplemented either with standard purified diet (SD) or whole-grain barley supplemented diet (WGB) for six weeks. Subsequently, (poly)phenolic metabolites were determined in urine samples by UPLC-MS/MS, and the principal metabolic pathways were elucidated. Thirty-nine (poly)phenolics compounds were identified in WGB which were distributed between the free (58%) and bound (42%) fractions, encompassing anthocyanins, phenolic acids, flavan-3-ols and flavones. Upon WGB intake, forty-two (poly)phenolic metabolites were identified, predominantly comprising phase-II sulphate, glucuronide, and/or methylated conjugates, along with colonic catabolites. Noteworthy metabolites included peonidin-3-O-glucuronide, peonidin-3-O-6′′-O-malonylglucoside, and peonidin-3-O-glucoside among anthocyanins; hydroxyphenylpropanoic acid-O-sulphate among phenolic acids; and 5-(3′,4′-dihydroxyphenyl)-γ-valerolactone-O-sulphate among flavan-3-ols. Metabolites like phenylpropionic, phenylacetic, hydroxybenzoic, and hippuric acids were found in both WGB and SD groups, with higher levels after barley consumption, indicating both endogenous and polyphenolic metabolism origins. Overall, this study offers valuable insights into the metabolism of (poly)phenols in purple barley, setting the stage for future investigations into the health benefits linked to the consumption of purple grain barley.
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1039/d4fo01275e
https://hdl.handle.net/10459.1/466849
url https://doi.org/10.1039/d4fo01275e
https://hdl.handle.net/10459.1/466849
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113009RB-I00
Reproducció del document publicat a https://doi.org/10.1039/d4fo01275e
Food and Function, 2024, vol.15, núm. 16, p. 8300-8309
https://doi.org/10.34810/data1387
dc.rights.none.fl_str_mv cc-by-nc (c) Cortijo-Alfonso et al., 2024
Attribution-NonCommercial 4.0 International
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc/4.0/
rights_invalid_str_mv cc-by-nc (c) Cortijo-Alfonso et al., 2024
Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:Repositori Obert UdL
instname:Universitat de Lleida (UdL)
instname_str Universitat de Lleida (UdL)
reponame_str Repositori Obert UdL
collection Repositori Obert UdL
repository.name.fl_str_mv
repository.mail.fl_str_mv
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