Study of the antimicrobial activity of cationic carbosilane dendrimers against clinical strains of multidrug-resistant bacteria and their biofilms

IntroductionAntimicrobial Resistance is a serious public health problem, which is aggravated by the ability of the microorganisms to form biofilms. Therefore, new therapeutic strategies need to be found, one of them being the use of cationic dendritic systems (dendrimers and dendrons).MethodsThe aim...

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Autores: Hernando Gozalo, Marcos|||0000-0003-2838-7270, Aguilera Correa, John Jairo, Rescalvo Casas, Carlos|||0000-0003-3745-6661, Seijas Pereda, Laura, García Bertolín, Carlos, Mata de la Mata, Francisco Javier de la|||0000-0003-0418-3935, Sánchez-Nieves Fernández, Javier|||0000-0003-0410-2285, Cuadros González, Juan|||0000-0003-4098-4377, Pérez Tanoira, Ramón|||0000-0002-9816-3208
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Universidad de Alcalá (UAH)
Repositorio:e_Buah Biblioteca Digital Universidad de Alcalá
Idioma:inglés
OAI Identifier:oai:ebuah.uah.es:10017/58416
Acceso en línea:http://hdl.handle.net/10017/58416
https://dx.doi.org/10.3389/fcimb.2023.1203991
Access Level:acceso abierto
Palabra clave:multiresistant bacteria
biofilms
antibacterial
carbosilane dendrimers and dendrons
quaternary ammonium salts
Chemistry
Química
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spelling Study of the antimicrobial activity of cationic carbosilane dendrimers against clinical strains of multidrug-resistant bacteria and their biofilmsHernando Gozalo, Marcos|||0000-0003-2838-7270Aguilera Correa, John JairoRescalvo Casas, Carlos|||0000-0003-3745-6661Seijas Pereda, LauraGarcía Bertolín, CarlosMata de la Mata, Francisco Javier de la|||0000-0003-0418-3935Sánchez-Nieves Fernández, Javier|||0000-0003-0410-2285Cuadros González, Juan|||0000-0003-4098-4377Pérez Tanoira, Ramón|||0000-0002-9816-3208multiresistant bacteriabiofilmsantibacterialcarbosilane dendrimers and dendronsquaternary ammonium saltsChemistryQuímicaIntroductionAntimicrobial Resistance is a serious public health problem, which is aggravated by the ability of the microorganisms to form biofilms. Therefore, new therapeutic strategies need to be found, one of them being the use of cationic dendritic systems (dendrimers and dendrons).MethodsThe aim of this study is to analyze the in vitro antimicrobial efficacy of six cationic carbosilane (CBS) dendrimers and one dendron with peripheral ammonium groups against multidrug-resistant bacteria, some of them isolated hospital strains, and their biofilms. For this purpose, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), minimum biofilm inhibitory concentration (MBIC) and minimum eradication biofilm concentration (MBEC) studies were carried out. In addition, the cytotoxicity on Hela cells of those compounds that proved to be the most effective was analyzed.ResultsAll the tested compounds showed in vitro activity against the planktonic forms of methicillin-resistant Staphylococcus aureus and only the dendrimers BDSQ017, BDAC-001 and BDLS-001 and the dendron BDEF-130 against their biofilms. On the other hand, only the dendrimers BDAC 001, BDLS-001 and BDJS-049 and the dendron BDEF-130 were antibacterial in vitro against the planktonic forms of multidrug-resistant Pseudomonas aeruginosa, but they lacked activity against their preformed biofilms. In addition, the dendrimers BDAC-001, BDLS-001 and BDSQ-017 and the dendron BDEF-130 exhibited a good profile of cytotoxicity in vitro.DiscussionOur study demonstrates the possibility of using the four compounds mentioned above as possible topical antimicrobials against the clinical and reference strains of multidrug-resistant bacteria.20232023-10-10journal articlehttp://purl.org/coar/resource_type/c_6501NAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10017/58416https://dx.doi.org/10.3389/fcimb.2023.1203991reponame:e_Buah Biblioteca Digital Universidad de Alcaláinstname:Universidad de Alcalá (UAH)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:ebuah.uah.es:10017/584162026-06-18T11:13:07Z
dc.title.none.fl_str_mv Study of the antimicrobial activity of cationic carbosilane dendrimers against clinical strains of multidrug-resistant bacteria and their biofilms
title Study of the antimicrobial activity of cationic carbosilane dendrimers against clinical strains of multidrug-resistant bacteria and their biofilms
spellingShingle Study of the antimicrobial activity of cationic carbosilane dendrimers against clinical strains of multidrug-resistant bacteria and their biofilms
Hernando Gozalo, Marcos|||0000-0003-2838-7270
multiresistant bacteria
biofilms
antibacterial
carbosilane dendrimers and dendrons
quaternary ammonium salts
Chemistry
Química
title_short Study of the antimicrobial activity of cationic carbosilane dendrimers against clinical strains of multidrug-resistant bacteria and their biofilms
title_full Study of the antimicrobial activity of cationic carbosilane dendrimers against clinical strains of multidrug-resistant bacteria and their biofilms
title_fullStr Study of the antimicrobial activity of cationic carbosilane dendrimers against clinical strains of multidrug-resistant bacteria and their biofilms
title_full_unstemmed Study of the antimicrobial activity of cationic carbosilane dendrimers against clinical strains of multidrug-resistant bacteria and their biofilms
title_sort Study of the antimicrobial activity of cationic carbosilane dendrimers against clinical strains of multidrug-resistant bacteria and their biofilms
dc.creator.none.fl_str_mv Hernando Gozalo, Marcos|||0000-0003-2838-7270
Aguilera Correa, John Jairo
Rescalvo Casas, Carlos|||0000-0003-3745-6661
Seijas Pereda, Laura
García Bertolín, Carlos
Mata de la Mata, Francisco Javier de la|||0000-0003-0418-3935
Sánchez-Nieves Fernández, Javier|||0000-0003-0410-2285
Cuadros González, Juan|||0000-0003-4098-4377
Pérez Tanoira, Ramón|||0000-0002-9816-3208
author Hernando Gozalo, Marcos|||0000-0003-2838-7270
author_facet Hernando Gozalo, Marcos|||0000-0003-2838-7270
Aguilera Correa, John Jairo
Rescalvo Casas, Carlos|||0000-0003-3745-6661
Seijas Pereda, Laura
García Bertolín, Carlos
Mata de la Mata, Francisco Javier de la|||0000-0003-0418-3935
Sánchez-Nieves Fernández, Javier|||0000-0003-0410-2285
Cuadros González, Juan|||0000-0003-4098-4377
Pérez Tanoira, Ramón|||0000-0002-9816-3208
author_role author
author2 Aguilera Correa, John Jairo
Rescalvo Casas, Carlos|||0000-0003-3745-6661
Seijas Pereda, Laura
García Bertolín, Carlos
Mata de la Mata, Francisco Javier de la|||0000-0003-0418-3935
Sánchez-Nieves Fernández, Javier|||0000-0003-0410-2285
Cuadros González, Juan|||0000-0003-4098-4377
Pérez Tanoira, Ramón|||0000-0002-9816-3208
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv multiresistant bacteria
biofilms
antibacterial
carbosilane dendrimers and dendrons
quaternary ammonium salts
Chemistry
Química
topic multiresistant bacteria
biofilms
antibacterial
carbosilane dendrimers and dendrons
quaternary ammonium salts
Chemistry
Química
description IntroductionAntimicrobial Resistance is a serious public health problem, which is aggravated by the ability of the microorganisms to form biofilms. Therefore, new therapeutic strategies need to be found, one of them being the use of cationic dendritic systems (dendrimers and dendrons).MethodsThe aim of this study is to analyze the in vitro antimicrobial efficacy of six cationic carbosilane (CBS) dendrimers and one dendron with peripheral ammonium groups against multidrug-resistant bacteria, some of them isolated hospital strains, and their biofilms. For this purpose, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), minimum biofilm inhibitory concentration (MBIC) and minimum eradication biofilm concentration (MBEC) studies were carried out. In addition, the cytotoxicity on Hela cells of those compounds that proved to be the most effective was analyzed.ResultsAll the tested compounds showed in vitro activity against the planktonic forms of methicillin-resistant Staphylococcus aureus and only the dendrimers BDSQ017, BDAC-001 and BDLS-001 and the dendron BDEF-130 against their biofilms. On the other hand, only the dendrimers BDAC 001, BDLS-001 and BDJS-049 and the dendron BDEF-130 were antibacterial in vitro against the planktonic forms of multidrug-resistant Pseudomonas aeruginosa, but they lacked activity against their preformed biofilms. In addition, the dendrimers BDAC-001, BDLS-001 and BDSQ-017 and the dendron BDEF-130 exhibited a good profile of cytotoxicity in vitro.DiscussionOur study demonstrates the possibility of using the four compounds mentioned above as possible topical antimicrobials against the clinical and reference strains of multidrug-resistant bacteria.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-10-10
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
NA
http://purl.org/coar/version/c_be7fb7dd8ff6fe43
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10017/58416
https://dx.doi.org/10.3389/fcimb.2023.1203991
url http://hdl.handle.net/10017/58416
https://dx.doi.org/10.3389/fcimb.2023.1203991
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:e_Buah Biblioteca Digital Universidad de Alcalá
instname:Universidad de Alcalá (UAH)
instname_str Universidad de Alcalá (UAH)
reponame_str e_Buah Biblioteca Digital Universidad de Alcalá
collection e_Buah Biblioteca Digital Universidad de Alcalá
repository.name.fl_str_mv
repository.mail.fl_str_mv
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