GPETAFLR, a biopeptide from Lupinus angustifolius L., protects against oxidative and inflammatory damage in retinal pigment epithelium cells

GPETAFLR, an octapeptide released from the enzymatic hydrolysis of lupine (Lupinus angustifolius L.) protein, has demonstrated anti‐inflammatory effect in myeloid lineage. This work aims to evaluate in retinal pigment epithelium (RPE) cells the protective role of GPETAFLR on both oxidative and infla...

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Detalles Bibliográficos
Autores: Millán-Linares, María del Carmen, Toscano-Sánchez, Rocío, Lemus-Conejo, Ana, Martín-Rubio, María E., Pedroche, Justo, Millán, Francisco, Montserrat-de la Paz, Sergio
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/198492
Acceso en línea:http://hdl.handle.net/10261/198492
Access Level:acceso abierto
Palabra clave:Age‐related macular degeneration
Biopeptide
Lupine seeds
Lupinus angustifolius
retinal pigment epithelium
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spelling GPETAFLR, a biopeptide from Lupinus angustifolius L., protects against oxidative and inflammatory damage in retinal pigment epithelium cellsMillán-Linares, María del CarmenMillán-Linares, María del CarmenToscano-Sánchez, RocíoLemus-Conejo, AnaMartín-Rubio, María E.Pedroche, JustoMillán, FranciscoMontserrat-de la Paz, SergioAge‐related macular degenerationBiopeptideLupine seedsLupinus angustifoliusretinal pigment epitheliumGPETAFLR, an octapeptide released from the enzymatic hydrolysis of lupine (Lupinus angustifolius L.) protein, has demonstrated anti‐inflammatory effect in myeloid lineage. This work aims to evaluate in retinal pigment epithelium (RPE) cells the protective role of GPETAFLR on both oxidative and inflammatory markers known to be involved in age‐related macular degeneration (AMD). In comparison with stimulated control cells, GPETAFLR increased glutathione production and diminished the secretion and gene expression of VEFG, IL‐1β, IL‐6, IFNγ, and TNF‐α, as well as reactive oxygen species, and nitrite output. Our findings reveal that GPETAFLR, a novel plant peptide, is able to protect against RPE oxidative stress and inflammation. Taken together, these results strongly support innovative nutritional strategies considering Lupinus angustifolius L. as source of proteins to prevent the onset and progression of AMD.his work was supported by grant AGL2012‐40247‐C02‐01 from the Spanish Ministry of Economy and Competitiveness. Authors thank Cell Biology Unit of Instituto de la Grasa for its assistance during the fulfilment of this work and Research Scientist Enrique Martínez Force for his help with the protein structure models. SMP acknowledges financial support from “V Own Research Plan” (University of Seville).Peer reviewedJohn Wiley & SonsMinisterio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202020202019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/198492reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1111/jfbc.12995Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1984922026-05-22T06:33:51Z
dc.title.none.fl_str_mv GPETAFLR, a biopeptide from Lupinus angustifolius L., protects against oxidative and inflammatory damage in retinal pigment epithelium cells
title GPETAFLR, a biopeptide from Lupinus angustifolius L., protects against oxidative and inflammatory damage in retinal pigment epithelium cells
spellingShingle GPETAFLR, a biopeptide from Lupinus angustifolius L., protects against oxidative and inflammatory damage in retinal pigment epithelium cells
Millán-Linares, María del Carmen
Age‐related macular degeneration
Biopeptide
Lupine seeds
Lupinus angustifolius
retinal pigment epithelium
title_short GPETAFLR, a biopeptide from Lupinus angustifolius L., protects against oxidative and inflammatory damage in retinal pigment epithelium cells
title_full GPETAFLR, a biopeptide from Lupinus angustifolius L., protects against oxidative and inflammatory damage in retinal pigment epithelium cells
title_fullStr GPETAFLR, a biopeptide from Lupinus angustifolius L., protects against oxidative and inflammatory damage in retinal pigment epithelium cells
title_full_unstemmed GPETAFLR, a biopeptide from Lupinus angustifolius L., protects against oxidative and inflammatory damage in retinal pigment epithelium cells
title_sort GPETAFLR, a biopeptide from Lupinus angustifolius L., protects against oxidative and inflammatory damage in retinal pigment epithelium cells
dc.creator.none.fl_str_mv Millán-Linares, María del Carmen
Millán-Linares, María del Carmen
Toscano-Sánchez, Rocío
Lemus-Conejo, Ana
Martín-Rubio, María E.
Pedroche, Justo
Millán, Francisco
Montserrat-de la Paz, Sergio
author Millán-Linares, María del Carmen
author_facet Millán-Linares, María del Carmen
Toscano-Sánchez, Rocío
Lemus-Conejo, Ana
Martín-Rubio, María E.
Pedroche, Justo
Millán, Francisco
Montserrat-de la Paz, Sergio
author_role author
author2 Toscano-Sánchez, Rocío
Lemus-Conejo, Ana
Martín-Rubio, María E.
Pedroche, Justo
Millán, Francisco
Montserrat-de la Paz, Sergio
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Age‐related macular degeneration
Biopeptide
Lupine seeds
Lupinus angustifolius
retinal pigment epithelium
topic Age‐related macular degeneration
Biopeptide
Lupine seeds
Lupinus angustifolius
retinal pigment epithelium
description GPETAFLR, an octapeptide released from the enzymatic hydrolysis of lupine (Lupinus angustifolius L.) protein, has demonstrated anti‐inflammatory effect in myeloid lineage. This work aims to evaluate in retinal pigment epithelium (RPE) cells the protective role of GPETAFLR on both oxidative and inflammatory markers known to be involved in age‐related macular degeneration (AMD). In comparison with stimulated control cells, GPETAFLR increased glutathione production and diminished the secretion and gene expression of VEFG, IL‐1β, IL‐6, IFNγ, and TNF‐α, as well as reactive oxygen species, and nitrite output. Our findings reveal that GPETAFLR, a novel plant peptide, is able to protect against RPE oxidative stress and inflammation. Taken together, these results strongly support innovative nutritional strategies considering Lupinus angustifolius L. as source of proteins to prevent the onset and progression of AMD.
publishDate 2019
dc.date.none.fl_str_mv 2019
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/198492
url http://hdl.handle.net/10261/198492
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1111/jfbc.12995
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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