3,4-Methylenedioxymethamphetamine (“Ecstasy”) Stimulates the Expression of a1(I) Procollagen mRNA in Hepatic Stellate Cells

3,4-Methylenedioxymethamphetamine, MDMA ("Ecstasy"), has been previously shown to produce cell necrosis and fibrosis in the liver. Our aim was to study the effect of MDMA on the type I collagen production by a cell line of hepatic stellate cells (HSC), the cell type mainly responsible for...

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Detalles Bibliográficos
Autores: Varela Rey, Marta María, Montiel-Duarte, Cristina, Beitia, G, Cenarruzabeitia, E, Iraburu, María José
Tipo de recurso: artículo
Fecha de publicación:1999
País:España
Institución:Universidad de Santiago de Compostela (USC)
Repositorio:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
Idioma:inglés
OAI Identifier:oai:minerva.usc.gal:10347/39363
Acceso en línea:https://hdl.handle.net/10347/39363
Access Level:acceso abierto
Palabra clave:MDMA
Type I collagen
Hepatic stellate cells
Glutathione
Liver fibrosis
Investigación
Descripción
Sumario:3,4-Methylenedioxymethamphetamine, MDMA ("Ecstasy"), has been previously shown to produce cell necrosis and fibrosis in the liver. Our aim was to study the effect of MDMA on the type I collagen production by a cell line of hepatic stellate cells (HSC), the cell type mainly responsible for collagen synthesis in the liver. We demonstrated that MDMA increases alpha1(I) procollagen mRNA levels and that this increase correlates with glutathione depletion and enhanced hydrogen peroxide production by HSC. Pre-treatment with either glutathione monoethyl ester or deferoxamine prevents the MDMA-induced alpha1(I) procollagen mRNA expression, indicating oxidative stress to be a mediator of this effect. Lipid peroxidation was not detected in MDMA-treated cells and therefore does not seem to be involved in the pro-fibrogenic action of MDMA on HSC.