Blocking ATP signaling and ROS generation preserves neurogenesis and network activity In epileptogenesis In hippocampal organotypic slice cultures.

227 p.

Detalles Bibliográficos
Autor: Rodríguez Bodero, Ane
Tipo de recurso: tesis doctoral
Fecha de publicación:2024
País:España
Institución:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/68143
Acceso en línea:http://hdl.handle.net/10810/68143
Access Level:acceso abierto
Palabra clave:cell biology
veterinary pathology
biología celular
patología veterinaria
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spelling Blocking ATP signaling and ROS generation preserves neurogenesis and network activity In epileptogenesis In hippocampal organotypic slice cultures.Rodríguez Bodero, Anecell biologyveterinary pathologybiología celularpatología veterinaria227 p.Epilepsy is a common neurological disorder, which lacks a comprehensive understanding of epileptogenesis. Temporal lobe epilepsy (TLE) in particular, often involves the hippocampus, with gliosis and abnormal neurogenesis. In our study, we aimed to evaluate and mitigate abnormal neurogenesis in TLE using an ex vivo model of hyperexcitable hippocampal organotypic culture slices (hOTCs) with disrupted GABAergic transmission by picrotoxin (PTX). Here, we observed that the epileptiform environment in hOTCs reduced newborn neuron density and impaired dendritic growth. Additionally, inhibiting newborn neurons restored normal firing patterns, indicating a link between neurogenesis and network activity. When we therefore targeted neuroinflammation in epileptogenesis through ATP signaling pathways by blocking purinergic receptors (P2XRs) or by reducing oxidative stress with cerium oxide nanoparticles, we observed recovery in most of the features studied. However, recovery in dendritic arborization occurred with P2XR inhibition but not in oxidative stress reduction. As a general conclusion, we observed that modulating ATP signaling prevented epileptiform activity, showcasing the importance of ATP and ROS in neurogenesis.Tonnesen, JanEncinas Pérez, Juan Manuel2024202420242024info:eu-repo/semantics/doctoralThesisapplication/pdfhttp://hdl.handle.net/10810/68143reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoInglésinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-sa/3.0/es/Atribución-CompartirIgual 3.0 España(cc)2024 ANE RODRIGUEZ BODERO (cc by-sa 4.0)oai:addi.ehu.eus:10810/681432026-06-18T09:23:17Z
dc.title.none.fl_str_mv Blocking ATP signaling and ROS generation preserves neurogenesis and network activity In epileptogenesis In hippocampal organotypic slice cultures.
title Blocking ATP signaling and ROS generation preserves neurogenesis and network activity In epileptogenesis In hippocampal organotypic slice cultures.
spellingShingle Blocking ATP signaling and ROS generation preserves neurogenesis and network activity In epileptogenesis In hippocampal organotypic slice cultures.
Rodríguez Bodero, Ane
cell biology
veterinary pathology
biología celular
patología veterinaria
title_short Blocking ATP signaling and ROS generation preserves neurogenesis and network activity In epileptogenesis In hippocampal organotypic slice cultures.
title_full Blocking ATP signaling and ROS generation preserves neurogenesis and network activity In epileptogenesis In hippocampal organotypic slice cultures.
title_fullStr Blocking ATP signaling and ROS generation preserves neurogenesis and network activity In epileptogenesis In hippocampal organotypic slice cultures.
title_full_unstemmed Blocking ATP signaling and ROS generation preserves neurogenesis and network activity In epileptogenesis In hippocampal organotypic slice cultures.
title_sort Blocking ATP signaling and ROS generation preserves neurogenesis and network activity In epileptogenesis In hippocampal organotypic slice cultures.
dc.creator.none.fl_str_mv Rodríguez Bodero, Ane
author Rodríguez Bodero, Ane
author_facet Rodríguez Bodero, Ane
author_role author
dc.contributor.none.fl_str_mv Tonnesen, Jan
Encinas Pérez, Juan Manuel
dc.subject.none.fl_str_mv cell biology
veterinary pathology
biología celular
patología veterinaria
topic cell biology
veterinary pathology
biología celular
patología veterinaria
description 227 p.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/68143
url http://hdl.handle.net/10810/68143
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-sa/3.0/es/
Atribución-CompartirIgual 3.0 España
(cc)2024 ANE RODRIGUEZ BODERO (cc by-sa 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-sa/3.0/es/
Atribución-CompartirIgual 3.0 España
(cc)2024 ANE RODRIGUEZ BODERO (cc by-sa 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
instname_str Universidad del País Vasco
reponame_str Addi. Archivo Digital para la Docencia y la Investigación
collection Addi. Archivo Digital para la Docencia y la Investigación
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301629

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