FLCCR is a fluorescent reporter system that quantifies the duration of different cell cycle phases at the single-cell level in fission yeast

Fission yeast is an excellent model system that has been widely used to study the mechanism that control cell cycle progression. However, there is a lack of tools that allow to measure with high precision the duration of the different phases of the cell cycle in individual cells. To circumvent this...

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Detalles Bibliográficos
Autores: Murciano-Julià, Guillem, Francos Cárdenas, Marina, Salat Canela, Clàudia, 1989-, Hidalgo Hernando, Elena
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10230/69674
Acceso en línea:http://hdl.handle.net/10230/69674
http://dx.doi.org/10.1371/journal.pbio.3002969
Access Level:acceso abierto
Palabra clave:Cell cycle and cell division
Nuclear membrane
Mitosis
Synthesis phase
G2 phase
Schizosaccharomyces pombe
G1 phase
Plasmid construction
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spelling FLCCR is a fluorescent reporter system that quantifies the duration of different cell cycle phases at the single-cell level in fission yeastMurciano-Julià, GuillemFrancos Cárdenas, MarinaSalat Canela, Clàudia, 1989-Hidalgo Hernando, ElenaCell cycle and cell divisionNuclear membraneMitosisSynthesis phaseG2 phaseSchizosaccharomyces pombeG1 phasePlasmid constructionFission yeast is an excellent model system that has been widely used to study the mechanism that control cell cycle progression. However, there is a lack of tools that allow to measure with high precision the duration of the different phases of the cell cycle in individual cells. To circumvent this problem, we have developed a fluorescent reporter that allows the quantification of the different phases of the cell cycle at the single-cell level in most genetic backgrounds. To prove the accuracy of this fluorescent reporter, we have tested the reporter in strains known to have a delay in the G1/S or G2/M transitions, confirming the strength and versatility of the system. An advantage of this reporter is that it eliminates the need for culture synchronization, avoiding stressing the cells. Using this reporter, we show that unperturbed cells lacking Sty1 have a standard cell cycle length and distribution and that the extended length of these cells is due to their increased cell growth rate but not to alterations in their cell cycle progression.This work was supported by grant PID2022-136449NB-I00 funded by MICIU/AEI/10.13039/501100011033 and ERDF/EU to J.A. and by Unidad de Excelencia Maria de Maeztu (CEX2018-000792-M) to J.A. and E.H. E.H. is a recipient of an ICREA Academia Award (Generalitat de Catalunya). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Public Library of Science (PLoS)202520252025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/69674http://dx.doi.org/10.1371/journal.pbio.3002969reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésPLoS Biol. 2025 Jan 7;23(1):e3002969info:eu-repo/grantAgreement/ES/3PE/PID2022-136449NB-I00info:eu-repo/grantAgreement/ES/2PE/CEX2018-000792-M© 2025 Murciano-Julià et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:10230/696742026-05-29T05:05:01Z
dc.title.none.fl_str_mv FLCCR is a fluorescent reporter system that quantifies the duration of different cell cycle phases at the single-cell level in fission yeast
title FLCCR is a fluorescent reporter system that quantifies the duration of different cell cycle phases at the single-cell level in fission yeast
spellingShingle FLCCR is a fluorescent reporter system that quantifies the duration of different cell cycle phases at the single-cell level in fission yeast
Murciano-Julià, Guillem
Cell cycle and cell division
Nuclear membrane
Mitosis
Synthesis phase
G2 phase
Schizosaccharomyces pombe
G1 phase
Plasmid construction
title_short FLCCR is a fluorescent reporter system that quantifies the duration of different cell cycle phases at the single-cell level in fission yeast
title_full FLCCR is a fluorescent reporter system that quantifies the duration of different cell cycle phases at the single-cell level in fission yeast
title_fullStr FLCCR is a fluorescent reporter system that quantifies the duration of different cell cycle phases at the single-cell level in fission yeast
title_full_unstemmed FLCCR is a fluorescent reporter system that quantifies the duration of different cell cycle phases at the single-cell level in fission yeast
title_sort FLCCR is a fluorescent reporter system that quantifies the duration of different cell cycle phases at the single-cell level in fission yeast
dc.creator.none.fl_str_mv Murciano-Julià, Guillem
Francos Cárdenas, Marina
Salat Canela, Clàudia, 1989-
Hidalgo Hernando, Elena
author Murciano-Julià, Guillem
author_facet Murciano-Julià, Guillem
Francos Cárdenas, Marina
Salat Canela, Clàudia, 1989-
Hidalgo Hernando, Elena
author_role author
author2 Francos Cárdenas, Marina
Salat Canela, Clàudia, 1989-
Hidalgo Hernando, Elena
author2_role author
author
author
dc.subject.none.fl_str_mv Cell cycle and cell division
Nuclear membrane
Mitosis
Synthesis phase
G2 phase
Schizosaccharomyces pombe
G1 phase
Plasmid construction
topic Cell cycle and cell division
Nuclear membrane
Mitosis
Synthesis phase
G2 phase
Schizosaccharomyces pombe
G1 phase
Plasmid construction
description Fission yeast is an excellent model system that has been widely used to study the mechanism that control cell cycle progression. However, there is a lack of tools that allow to measure with high precision the duration of the different phases of the cell cycle in individual cells. To circumvent this problem, we have developed a fluorescent reporter that allows the quantification of the different phases of the cell cycle at the single-cell level in most genetic backgrounds. To prove the accuracy of this fluorescent reporter, we have tested the reporter in strains known to have a delay in the G1/S or G2/M transitions, confirming the strength and versatility of the system. An advantage of this reporter is that it eliminates the need for culture synchronization, avoiding stressing the cells. Using this reporter, we show that unperturbed cells lacking Sty1 have a standard cell cycle length and distribution and that the extended length of these cells is due to their increased cell growth rate but not to alterations in their cell cycle progression.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/69674
http://dx.doi.org/10.1371/journal.pbio.3002969
url http://hdl.handle.net/10230/69674
http://dx.doi.org/10.1371/journal.pbio.3002969
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv PLoS Biol. 2025 Jan 7;23(1):e3002969
info:eu-repo/grantAgreement/ES/3PE/PID2022-136449NB-I00
info:eu-repo/grantAgreement/ES/2PE/CEX2018-000792-M
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science (PLoS)
publisher.none.fl_str_mv Public Library of Science (PLoS)
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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