Optimization of fourteen microsatellite loci in a Mediterranean demosponge subjected to population decimation, Ircinia fasciculata

The recovery potential of decimated populations of sponges will largely hinge on their populations' size retrieval and their connectivity with conspecifics in unaffected locations. Here, we report on the development of microsatellite markers for estimation of the population connectivity and bot...

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Detalhes bibliográficos
Autores: Riesgo Gil, Ana, Blasco, Gema, Erwin, P.M., Pérez Portela, Rocío, López Legentil, Susanna
Formato: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2014
País:España
Recursos:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/165578
Acesso em linha:https://hdl.handle.net/2445/165578
Access Level:acceso abierto
Palavra-chave:Esponges
Reproducció
Epidèmies
Sponges
Reproduction
Epidemics
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repository_id_str
spelling Optimization of fourteen microsatellite loci in a Mediterranean demosponge subjected to population decimation, Ircinia fasciculataRiesgo Gil, AnaBlasco, GemaErwin, P.M.Pérez Portela, RocíoLópez Legentil, SusannaEspongesReproduccióEpidèmiesSpongesReproductionEpidemicsThe recovery potential of decimated populations of sponges will largely hinge on their populations' size retrieval and their connectivity with conspecifics in unaffected locations. Here, we report on the development of microsatellite markers for estimation of the population connectivity and bottleneck and inbreeding signals in a Mediterranean sponge suffering from disease outbreaks, Ircinia fasciculata. From the 220,876 sequences obtained by genomic pyrosequencing, we isolated 14 polymorphic microsatellite loci and assessed the allelic variation of loci in 24 individuals from 2 populations in the Northwestern Mediterranean. The allele number per locus ranged from 3 to 11, observed heterozygosity from 0.68 to 0.73, and expected heterozygosity from 0.667 to 0.68. No significant linkage disequilibrium between pairs of loci was detected. The 14 markers developed here will be valuable tools for conservation strategies across the distributional range of this species allowing the detection of populations with large genetic diversity loss and high levels of inbreeding.Springer Science + Business Media2014info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://hdl.handle.net/2445/165578Articles publicats en revistes (Biologia Evolutiva, Ecologia i Ciències Ambientals)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésVersió postprint del document publicat a: https://doi.org/10.1007/s12686-013-0137-9Conservation Genetics Resources, 2014, vol. 6, num. 2, p. 301-303https://doi.org/10.1007/s12686-013-0137-9(c) Springer Science + Business Media, 2014info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1655782026-05-27T06:46:51Z
dc.title.none.fl_str_mv Optimization of fourteen microsatellite loci in a Mediterranean demosponge subjected to population decimation, Ircinia fasciculata
title Optimization of fourteen microsatellite loci in a Mediterranean demosponge subjected to population decimation, Ircinia fasciculata
spellingShingle Optimization of fourteen microsatellite loci in a Mediterranean demosponge subjected to population decimation, Ircinia fasciculata
Riesgo Gil, Ana
Esponges
Reproducció
Epidèmies
Sponges
Reproduction
Epidemics
title_short Optimization of fourteen microsatellite loci in a Mediterranean demosponge subjected to population decimation, Ircinia fasciculata
title_full Optimization of fourteen microsatellite loci in a Mediterranean demosponge subjected to population decimation, Ircinia fasciculata
title_fullStr Optimization of fourteen microsatellite loci in a Mediterranean demosponge subjected to population decimation, Ircinia fasciculata
title_full_unstemmed Optimization of fourteen microsatellite loci in a Mediterranean demosponge subjected to population decimation, Ircinia fasciculata
title_sort Optimization of fourteen microsatellite loci in a Mediterranean demosponge subjected to population decimation, Ircinia fasciculata
dc.creator.none.fl_str_mv Riesgo Gil, Ana
Blasco, Gema
Erwin, P.M.
Pérez Portela, Rocío
López Legentil, Susanna
author Riesgo Gil, Ana
author_facet Riesgo Gil, Ana
Blasco, Gema
Erwin, P.M.
Pérez Portela, Rocío
López Legentil, Susanna
author_role author
author2 Blasco, Gema
Erwin, P.M.
Pérez Portela, Rocío
López Legentil, Susanna
author2_role author
author
author
author
dc.subject.none.fl_str_mv Esponges
Reproducció
Epidèmies
Sponges
Reproduction
Epidemics
topic Esponges
Reproducció
Epidèmies
Sponges
Reproduction
Epidemics
description The recovery potential of decimated populations of sponges will largely hinge on their populations' size retrieval and their connectivity with conspecifics in unaffected locations. Here, we report on the development of microsatellite markers for estimation of the population connectivity and bottleneck and inbreeding signals in a Mediterranean sponge suffering from disease outbreaks, Ircinia fasciculata. From the 220,876 sequences obtained by genomic pyrosequencing, we isolated 14 polymorphic microsatellite loci and assessed the allelic variation of loci in 24 individuals from 2 populations in the Northwestern Mediterranean. The allele number per locus ranged from 3 to 11, observed heterozygosity from 0.68 to 0.73, and expected heterozygosity from 0.667 to 0.68. No significant linkage disequilibrium between pairs of loci was detected. The 14 markers developed here will be valuable tools for conservation strategies across the distributional range of this species allowing the detection of populations with large genetic diversity loss and high levels of inbreeding.
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/165578
url https://hdl.handle.net/2445/165578
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1007/s12686-013-0137-9
Conservation Genetics Resources, 2014, vol. 6, num. 2, p. 301-303
https://doi.org/10.1007/s12686-013-0137-9
dc.rights.none.fl_str_mv (c) Springer Science + Business Media, 2014
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Springer Science + Business Media, 2014
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Science + Business Media
publisher.none.fl_str_mv Springer Science + Business Media
dc.source.none.fl_str_mv Articles publicats en revistes (Biologia Evolutiva, Ecologia i Ciències Ambientals)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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