A reverse vaccinology approach to the identification and characterization of Ctenocephalides felis candidate protective antigens for the control of cat flea infestations

[Background]: Despite the abundance of the domestic cat flea, Ctenocephalides felis (Bouché, 1835) and disease risks associated with them, flea control is difficult and requires the development of new control interventions such as vaccines. In this study, a reverse vaccinology approach was designed...

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Autores: Contreras, Marinela, Villar, Margarita, Artigas-Jerónimo, Sara, Kornieieva, Lidiia, Mytrofanov, Sergii, Fuente, José de la
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/174467
Acceso en línea:http://hdl.handle.net/10261/174467
Access Level:acceso abierto
Palabra clave:Immunology
Flea
Proteomics
Transcriptomics
Vaccine
Vaccinology
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spelling A reverse vaccinology approach to the identification and characterization of Ctenocephalides felis candidate protective antigens for the control of cat flea infestationsContreras, MarinelaVillar, MargaritaArtigas-Jerónimo, SaraKornieieva, LidiiaMytrofanov, SergiiFuente, José de laImmunologyFleaProteomicsTranscriptomicsVaccineVaccinology[Background]: Despite the abundance of the domestic cat flea, Ctenocephalides felis (Bouché, 1835) and disease risks associated with them, flea control is difficult and requires the development of new control interventions such as vaccines. In this study, a reverse vaccinology approach was designed to achieve a rational selection of cat flea candidate protective antigens. [Methods]: Based on transcriptomics and proteomics data from unfed adult fleas it was possible to select more specific candidate protective antigens based on highly represented and functionally relevant proteins present in the predicted exoproteome. The protective capacity of the recombinant antigens was evaluated for the control of C. felis infestations in vaccinated cats. [Results]: Vaccination with recombinant antigens induced an antibody response in immunized cats. Furthermore, a correlation was obtained between the effect of vaccination (antibody levels) and vaccine efficacy on flea phenotype (egg hatchability). The results suggested that the main effect of vaccination with these antigens was on reducing cat flea egg hatchability and fertility, with an overall vaccine efficacy of 32-46%. Although vaccination with these antigens did not have an effect on flea infestations, vaccines affecting reproductive capacity could reduce cat flea populations, particularly under conditions of direct insect transmission between cats. [Conclusions]: These results support the development of vaccines with protective antigens affecting flea reproduction and development after feeding on immunized animals for the control of cat flea infestations.This research was partially supported by CSIC grant 201440E098 to JF.Peer ReviewedBioMed CentralConsejo Superior de Investigaciones Científicas (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2019201920182019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/174467reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1186/s13071-018-2618-xSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1744672026-05-22T06:33:51Z
dc.title.none.fl_str_mv A reverse vaccinology approach to the identification and characterization of Ctenocephalides felis candidate protective antigens for the control of cat flea infestations
title A reverse vaccinology approach to the identification and characterization of Ctenocephalides felis candidate protective antigens for the control of cat flea infestations
spellingShingle A reverse vaccinology approach to the identification and characterization of Ctenocephalides felis candidate protective antigens for the control of cat flea infestations
Contreras, Marinela
Immunology
Flea
Proteomics
Transcriptomics
Vaccine
Vaccinology
title_short A reverse vaccinology approach to the identification and characterization of Ctenocephalides felis candidate protective antigens for the control of cat flea infestations
title_full A reverse vaccinology approach to the identification and characterization of Ctenocephalides felis candidate protective antigens for the control of cat flea infestations
title_fullStr A reverse vaccinology approach to the identification and characterization of Ctenocephalides felis candidate protective antigens for the control of cat flea infestations
title_full_unstemmed A reverse vaccinology approach to the identification and characterization of Ctenocephalides felis candidate protective antigens for the control of cat flea infestations
title_sort A reverse vaccinology approach to the identification and characterization of Ctenocephalides felis candidate protective antigens for the control of cat flea infestations
dc.creator.none.fl_str_mv Contreras, Marinela
Villar, Margarita
Artigas-Jerónimo, Sara
Kornieieva, Lidiia
Mytrofanov, Sergii
Fuente, José de la
author Contreras, Marinela
author_facet Contreras, Marinela
Villar, Margarita
Artigas-Jerónimo, Sara
Kornieieva, Lidiia
Mytrofanov, Sergii
Fuente, José de la
author_role author
author2 Villar, Margarita
Artigas-Jerónimo, Sara
Kornieieva, Lidiia
Mytrofanov, Sergii
Fuente, José de la
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Immunology
Flea
Proteomics
Transcriptomics
Vaccine
Vaccinology
topic Immunology
Flea
Proteomics
Transcriptomics
Vaccine
Vaccinology
description [Background]: Despite the abundance of the domestic cat flea, Ctenocephalides felis (Bouché, 1835) and disease risks associated with them, flea control is difficult and requires the development of new control interventions such as vaccines. In this study, a reverse vaccinology approach was designed to achieve a rational selection of cat flea candidate protective antigens. [Methods]: Based on transcriptomics and proteomics data from unfed adult fleas it was possible to select more specific candidate protective antigens based on highly represented and functionally relevant proteins present in the predicted exoproteome. The protective capacity of the recombinant antigens was evaluated for the control of C. felis infestations in vaccinated cats. [Results]: Vaccination with recombinant antigens induced an antibody response in immunized cats. Furthermore, a correlation was obtained between the effect of vaccination (antibody levels) and vaccine efficacy on flea phenotype (egg hatchability). The results suggested that the main effect of vaccination with these antigens was on reducing cat flea egg hatchability and fertility, with an overall vaccine efficacy of 32-46%. Although vaccination with these antigens did not have an effect on flea infestations, vaccines affecting reproductive capacity could reduce cat flea populations, particularly under conditions of direct insect transmission between cats. [Conclusions]: These results support the development of vaccines with protective antigens affecting flea reproduction and development after feeding on immunized animals for the control of cat flea infestations.
publishDate 2018
dc.date.none.fl_str_mv 2018
2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/174467
url http://hdl.handle.net/10261/174467
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1186/s13071-018-2618-x
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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