The functional differences between paralogous regulators define the control of the general stress response in Sphingopyxis granuli TFA

Sphingopyxis granuli TFA is a contaminant degrading alphaproteobacterium that responds to adverse conditions by inducing the general stress response (GSR), an adaptive response that controls the transcription of a variety of genes to overcome adverse conditions. The core GSR regulators (the response...

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Detalles Bibliográficos
Autores: Dios Barranco, Rubén de, Santero, Eduardo, Reyes-Ramírez, Francisca
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universidad Pablo de Olavide (UPO)
Repositorio:RIO. Repositorio Institucional Olavide
Idioma:inglés
OAI Identifier:oai:rio.upo.es:10433/25381
Acceso en línea:https://hdl.handle.net/10433/25381
Access Level:acceso abierto
Palabra clave:General stress response
Sigma factors
Signal transduction
Gene regulation
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spelling The functional differences between paralogous regulators define the control of the general stress response in Sphingopyxis granuli TFADios Barranco, Rubén deSantero, EduardoReyes-Ramírez, FranciscaGeneral stress responseSigma factorsSignal transductionGene regulationSphingopyxis granuli TFA is a contaminant degrading alphaproteobacterium that responds to adverse conditions by inducing the general stress response (GSR), an adaptive response that controls the transcription of a variety of genes to overcome adverse conditions. The core GSR regulators (the response regulator PhyR, the anti-σ factor NepR and the σ factor EcfG) are duplicated in TFA, being PhyR1 and PhyR2, NepR1 and NepR2 and EcfG1 and EcfG2. Based on multiple genetic, phenotypical and biochemical evidences including in vitro transcription assays, we have assigned distinct functional features to each paralogue and assessed their contribution to the GSR regulation, dictating its timing and the intensity. We show that different stress signals are differentially integrated into the GSR by PhyR1 and PhyR2, therefore producing different levels of GSR activation. We demonstrate in vitro that both NepR1 and NepR2 bind EcfG1 and EcfG2, although NepR1 produces a more stable interaction than NepR2. Conversely, NepR2 interacts with phosphorylated PhyR1 and PhyR2 more efficiently than NepR1. We propose an integrative model where NepR2 would play a dual negative role: it would directly inhibit the σ factors upon activation of the GSR and it would modulate the GSR activity indirectly by titrating the PhyR regulators.John Wiley & Sons Ltd20262026-01-0920222022-01-1320222022-01-13journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10433/25381reponame:RIO. Repositorio Institucional Olavideinstname:Universidad Pablo de Olavide (UPO)InglésengAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Not available Not availableNot available Not available Not availableopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:rio.upo.es:10433/253812026-06-13T12:46:27Z
dc.title.none.fl_str_mv The functional differences between paralogous regulators define the control of the general stress response in Sphingopyxis granuli TFA
title The functional differences between paralogous regulators define the control of the general stress response in Sphingopyxis granuli TFA
spellingShingle The functional differences between paralogous regulators define the control of the general stress response in Sphingopyxis granuli TFA
Dios Barranco, Rubén de
General stress response
Sigma factors
Signal transduction
Gene regulation
title_short The functional differences between paralogous regulators define the control of the general stress response in Sphingopyxis granuli TFA
title_full The functional differences between paralogous regulators define the control of the general stress response in Sphingopyxis granuli TFA
title_fullStr The functional differences between paralogous regulators define the control of the general stress response in Sphingopyxis granuli TFA
title_full_unstemmed The functional differences between paralogous regulators define the control of the general stress response in Sphingopyxis granuli TFA
title_sort The functional differences between paralogous regulators define the control of the general stress response in Sphingopyxis granuli TFA
dc.creator.none.fl_str_mv Dios Barranco, Rubén de
Santero, Eduardo
Reyes-Ramírez, Francisca
author Dios Barranco, Rubén de
author_facet Dios Barranco, Rubén de
Santero, Eduardo
Reyes-Ramírez, Francisca
author_role author
author2 Santero, Eduardo
Reyes-Ramírez, Francisca
author2_role author
author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv General stress response
Sigma factors
Signal transduction
Gene regulation
topic General stress response
Sigma factors
Signal transduction
Gene regulation
description Sphingopyxis granuli TFA is a contaminant degrading alphaproteobacterium that responds to adverse conditions by inducing the general stress response (GSR), an adaptive response that controls the transcription of a variety of genes to overcome adverse conditions. The core GSR regulators (the response regulator PhyR, the anti-σ factor NepR and the σ factor EcfG) are duplicated in TFA, being PhyR1 and PhyR2, NepR1 and NepR2 and EcfG1 and EcfG2. Based on multiple genetic, phenotypical and biochemical evidences including in vitro transcription assays, we have assigned distinct functional features to each paralogue and assessed their contribution to the GSR regulation, dictating its timing and the intensity. We show that different stress signals are differentially integrated into the GSR by PhyR1 and PhyR2, therefore producing different levels of GSR activation. We demonstrate in vitro that both NepR1 and NepR2 bind EcfG1 and EcfG2, although NepR1 produces a more stable interaction than NepR2. Conversely, NepR2 interacts with phosphorylated PhyR1 and PhyR2 more efficiently than NepR1. We propose an integrative model where NepR2 would play a dual negative role: it would directly inhibit the σ factors upon activation of the GSR and it would modulate the GSR activity indirectly by titrating the PhyR regulators.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-01-13
2022
2022-01-13
2026
2026-01-09
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10433/25381
url https://hdl.handle.net/10433/25381
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Not available Not available
Not available Not available Not available
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons Ltd
publisher.none.fl_str_mv John Wiley & Sons Ltd
dc.source.none.fl_str_mv reponame:RIO. Repositorio Institucional Olavide
instname:Universidad Pablo de Olavide (UPO)
instname_str Universidad Pablo de Olavide (UPO)
reponame_str RIO. Repositorio Institucional Olavide
collection RIO. Repositorio Institucional Olavide
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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