Immunization with LytB protein of Streptococcus pneumoniae activates complement-mediated phagocytosis and induces protection against pneumonia and sepsis

The cell wall glucosaminidase LytB of Streptococcus pneumoniae is a surface exposed protein involved in daughter cell separation, biofilm formation and contributes to different aspects of the pathogenesis process. In this study we have characterized the antibody responses after immunization of mice...

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Detalhes bibliográficos
Autores: Ruiz, Susana, Domenech, Mirian, Antequera, María Luisa, García, Pedro, García, Ernesto, Corsini, Bruno, Aguinagalde, Leire, Fenoll, Asuncion, Yuste, Jose Enrique
Formato: artículo
Fecha de publicación:2016
País:España
Recursos:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/10341
Acesso em linha:http://hdl.handle.net/20.500.12105/10341
Access Level:acceso abierto
Palavra-chave:Phagocytosis
Adjuvants, Immunologic
Aluminum Hydroxide
Animals
Antibodies, Bacterial
Complement System Proteins
Disease Models, Animal
Enzyme-Linked Immunosorbent Assay
Female
Immunoglobulin G
Immunologic Factors
Male
Mice, Inbred BALB C
N-Acetylmuramoyl-L-alanine Amidase
Neutrophils
Pneumococcal Infections
Pneumococcal Vaccines
Pneumonia, Bacterial
Sepsis
Streptococcus pneumoniae
Treatment Outcome
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spelling Immunization with LytB protein of Streptococcus pneumoniae activates complement-mediated phagocytosis and induces protection against pneumonia and sepsisRuiz, SusanaDomenech, MirianAntequera, María LuisaGarcía, PedroGarcía, ErnestoCorsini, BrunoAguinagalde, LeireFenoll, AsuncionYuste, Jose EnriquePhagocytosisAdjuvants, ImmunologicAluminum HydroxideAnimalsAntibodies, BacterialComplement System ProteinsDisease Models, AnimalEnzyme-Linked Immunosorbent AssayFemaleImmunoglobulin GImmunologic FactorsMaleMice, Inbred BALB CN-Acetylmuramoyl-L-alanine AmidaseNeutrophilsPneumococcal InfectionsPneumococcal VaccinesPneumonia, BacterialSepsisStreptococcus pneumoniaeTreatment OutcomeThe cell wall glucosaminidase LytB of Streptococcus pneumoniae is a surface exposed protein involved in daughter cell separation, biofilm formation and contributes to different aspects of the pathogenesis process. In this study we have characterized the antibody responses after immunization of mice with LytB in the presence of alhydrogel as an adjuvant. Enzyme-linked immunosorbent assays measuring different subclasses of immunoglobulin G, demonstrated that the antibody responses to LytB were predominantly IgG1 and IgG2b, followed by IgG3 and IgG2a subclasses. Complement-mediated immunity against two different pneumococcal serotypes was investigated using sera from immunized mice. Immunization with LytB increased the recognition of S. pneumoniae by complement components C1q and C3b demonstrating that anti-LytB antibodies trigger activation of the classical pathway. Phagocytosis assays showed that serum containing antibodies to LytB stimulates neutrophil-mediated phagocytosis against S. pneumoniae. Animal models of infection including invasive pneumonia and sepsis were performed with two different clinical isolates. Vaccination with LytB increased bacterial clearance and induced protection demonstrating that LytB might be a good candidate to be considered in a future protein-based vaccine against S. pneumoniae.ElsevierMinisterio de Economía y Competitividad (España)Centro de Investigación Biomedica en Red - CIBERNational Council for Scientific and Technological Development (Brasil)20202020-06-1120162016-01-0120162016-01-01journal articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/20.500.12105/10341reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/103412026-06-12T12:43:37Z
dc.title.none.fl_str_mv Immunization with LytB protein of Streptococcus pneumoniae activates complement-mediated phagocytosis and induces protection against pneumonia and sepsis
title Immunization with LytB protein of Streptococcus pneumoniae activates complement-mediated phagocytosis and induces protection against pneumonia and sepsis
spellingShingle Immunization with LytB protein of Streptococcus pneumoniae activates complement-mediated phagocytosis and induces protection against pneumonia and sepsis
Ruiz, Susana
Phagocytosis
Adjuvants, Immunologic
Aluminum Hydroxide
Animals
Antibodies, Bacterial
Complement System Proteins
Disease Models, Animal
Enzyme-Linked Immunosorbent Assay
Female
Immunoglobulin G
Immunologic Factors
Male
Mice, Inbred BALB C
N-Acetylmuramoyl-L-alanine Amidase
Neutrophils
Pneumococcal Infections
Pneumococcal Vaccines
Pneumonia, Bacterial
Sepsis
Streptococcus pneumoniae
Treatment Outcome
title_short Immunization with LytB protein of Streptococcus pneumoniae activates complement-mediated phagocytosis and induces protection against pneumonia and sepsis
title_full Immunization with LytB protein of Streptococcus pneumoniae activates complement-mediated phagocytosis and induces protection against pneumonia and sepsis
title_fullStr Immunization with LytB protein of Streptococcus pneumoniae activates complement-mediated phagocytosis and induces protection against pneumonia and sepsis
title_full_unstemmed Immunization with LytB protein of Streptococcus pneumoniae activates complement-mediated phagocytosis and induces protection against pneumonia and sepsis
title_sort Immunization with LytB protein of Streptococcus pneumoniae activates complement-mediated phagocytosis and induces protection against pneumonia and sepsis
dc.creator.none.fl_str_mv Ruiz, Susana
Domenech, Mirian
Antequera, María Luisa
García, Pedro
García, Ernesto
Corsini, Bruno
Aguinagalde, Leire
Fenoll, Asuncion
Yuste, Jose Enrique
author Ruiz, Susana
author_facet Ruiz, Susana
Domenech, Mirian
Antequera, María Luisa
García, Pedro
García, Ernesto
Corsini, Bruno
Aguinagalde, Leire
Fenoll, Asuncion
Yuste, Jose Enrique
author_role author
author2 Domenech, Mirian
Antequera, María Luisa
García, Pedro
García, Ernesto
Corsini, Bruno
Aguinagalde, Leire
Fenoll, Asuncion
Yuste, Jose Enrique
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Centro de Investigación Biomedica en Red - CIBER
National Council for Scientific and Technological Development (Brasil)
dc.subject.none.fl_str_mv Phagocytosis
Adjuvants, Immunologic
Aluminum Hydroxide
Animals
Antibodies, Bacterial
Complement System Proteins
Disease Models, Animal
Enzyme-Linked Immunosorbent Assay
Female
Immunoglobulin G
Immunologic Factors
Male
Mice, Inbred BALB C
N-Acetylmuramoyl-L-alanine Amidase
Neutrophils
Pneumococcal Infections
Pneumococcal Vaccines
Pneumonia, Bacterial
Sepsis
Streptococcus pneumoniae
Treatment Outcome
topic Phagocytosis
Adjuvants, Immunologic
Aluminum Hydroxide
Animals
Antibodies, Bacterial
Complement System Proteins
Disease Models, Animal
Enzyme-Linked Immunosorbent Assay
Female
Immunoglobulin G
Immunologic Factors
Male
Mice, Inbred BALB C
N-Acetylmuramoyl-L-alanine Amidase
Neutrophils
Pneumococcal Infections
Pneumococcal Vaccines
Pneumonia, Bacterial
Sepsis
Streptococcus pneumoniae
Treatment Outcome
description The cell wall glucosaminidase LytB of Streptococcus pneumoniae is a surface exposed protein involved in daughter cell separation, biofilm formation and contributes to different aspects of the pathogenesis process. In this study we have characterized the antibody responses after immunization of mice with LytB in the presence of alhydrogel as an adjuvant. Enzyme-linked immunosorbent assays measuring different subclasses of immunoglobulin G, demonstrated that the antibody responses to LytB were predominantly IgG1 and IgG2b, followed by IgG3 and IgG2a subclasses. Complement-mediated immunity against two different pneumococcal serotypes was investigated using sera from immunized mice. Immunization with LytB increased the recognition of S. pneumoniae by complement components C1q and C3b demonstrating that anti-LytB antibodies trigger activation of the classical pathway. Phagocytosis assays showed that serum containing antibodies to LytB stimulates neutrophil-mediated phagocytosis against S. pneumoniae. Animal models of infection including invasive pneumonia and sepsis were performed with two different clinical isolates. Vaccination with LytB increased bacterial clearance and induced protection demonstrating that LytB might be a good candidate to be considered in a future protein-based vaccine against S. pneumoniae.
publishDate 2016
dc.date.none.fl_str_mv 2016
2016-01-01
2016
2016-01-01
2020
2020-06-11
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12105/10341
url http://hdl.handle.net/20.500.12105/10341
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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