CPEB4 replaces CPEB1 to complete meiosis

In vertebrate oocytes, meiotic progression is driven by the sequential translational activation of maternal messenger RNAs stored in the cytoplasm. This activation is mainly induced by the cytoplasmic elongation of their poly(A) tails, which is mediated by the cytoplasmic polyadenylation element (CP...

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Detalles Bibliográficos
Autor: Igea Fernández, Ana
Tipo de recurso: tesis doctoral
Estado:Versión publicada
Fecha de publicación:2009
País:España
Institución:CBUC, CESCA
Repositorio:TDR. Tesis Doctorales en Red
OAI Identifier:oai:www.tdx.cat:10803/22687
Acceso en línea:http://hdl.handle.net/10803/22687
Access Level:acceso abierto
Palabra clave:maternal mRNAs
Cyclin B1
polyadenylation
3’UTR
translational control
Oocyte maturation
CPEB4
CPEB1
feedback loop
Xenopus laevis
576
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oai_identifier_str oai:www.tdx.cat:10803/22687
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repository_id_str
spelling CPEB4 replaces CPEB1 to complete meiosisIgea Fernández, Anamaternal mRNAsCyclin B1polyadenylation3’UTRtranslational controlOocyte maturationCPEB4CPEB1feedback loopXenopus laevis576In vertebrate oocytes, meiotic progression is driven by the sequential translational activation of maternal messenger RNAs stored in the cytoplasm. This activation is mainly induced by the cytoplasmic elongation of their poly(A) tails, which is mediated by the cytoplasmic polyadenylation element (CPE) present in their 3’ untranslated regions (3´ UTRs). Sequential, phase-specific translation of these maternal mRNAs is required to complete the two meiotic divisions. Although the earlier polyadenylation events in prophase I and metaphase I are driven by the CPE-binding protein 1 (CPEB1), 90% of this protein is degraded by the anaphase promoting complex in the first meiotic division. The low levels of CPEB1 during interkinesis and in metaphase II raise the question of how the cytoplasmic polyadenylation required for the second meiotic division is achieved. In this work, we demonstrate that CPEB1 activates the translation of the maternal mRNA encoding CPEB4, which, in turn, recruits the cytoplasmic poly(A) polymerase GLD2 to “late” CPE-regulated mRNAs driving the transition from metaphase I to metaphase II, and, therefore, replacing CPEB1 for “late” meiosis polyadenylation.Programa de doctorat en BiomedicinaUniversitat Pompeu FabraMéndez de la Iglesia, RaúlUniversitat Pompeu Fabra. Departament de Ciències Experimentals i de la Salut201120112009info:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/publishedVersion224 p.application/pdfapplication/pdfhttp://hdl.handle.net/10803/22687TDX (Tesis Doctorals en Xarxa)reponame:TDR. Tesis Doctorales en Redinstname:CBUC, CESCAInglésADVERTIMENT. L'accés als continguts d'aquesta tesi doctoral i la seva utilització ha de respectar els drets de la persona autora. Pot ser utilitzada per a consulta o estudi personal, així com en activitats o materials d'investigació i docència en els termes establerts a l'art. 32 del Text Refós de la Llei de Propietat Intel·lectual (RDL 1/1996). Per altres utilitzacions es requereix l'autorització prèvia i expressa de la persona autora. En qualsevol cas, en la utilització dels seus continguts caldrà indicar de forma clara el nom i cognoms de la persona autora i el títol de la tesi doctoral. No s'autoritza la seva reproducció o altres formes d'explotació efectuades amb finalitats de lucre ni la seva comunicació pública des d'un lloc aliè al servei TDX. Tampoc s'autoritza la presentació del seu contingut en una finestra o marc aliè a TDX (framing). Aquesta reserva de drets afecta tant als continguts de la tesi com als seus resums i índexs.info:eu-repo/semantics/openAccessoai:www.tdx.cat:10803/226872026-06-14T12:46:07Z
dc.title.none.fl_str_mv CPEB4 replaces CPEB1 to complete meiosis
title CPEB4 replaces CPEB1 to complete meiosis
spellingShingle CPEB4 replaces CPEB1 to complete meiosis
Igea Fernández, Ana
maternal mRNAs
Cyclin B1
polyadenylation
3’UTR
translational control
Oocyte maturation
CPEB4
CPEB1
feedback loop
Xenopus laevis
576
title_short CPEB4 replaces CPEB1 to complete meiosis
title_full CPEB4 replaces CPEB1 to complete meiosis
title_fullStr CPEB4 replaces CPEB1 to complete meiosis
title_full_unstemmed CPEB4 replaces CPEB1 to complete meiosis
title_sort CPEB4 replaces CPEB1 to complete meiosis
dc.creator.none.fl_str_mv Igea Fernández, Ana
author Igea Fernández, Ana
author_facet Igea Fernández, Ana
author_role author
dc.contributor.none.fl_str_mv Méndez de la Iglesia, Raúl
Universitat Pompeu Fabra. Departament de Ciències Experimentals i de la Salut
dc.subject.none.fl_str_mv maternal mRNAs
Cyclin B1
polyadenylation
3’UTR
translational control
Oocyte maturation
CPEB4
CPEB1
feedback loop
Xenopus laevis
576
topic maternal mRNAs
Cyclin B1
polyadenylation
3’UTR
translational control
Oocyte maturation
CPEB4
CPEB1
feedback loop
Xenopus laevis
576
description In vertebrate oocytes, meiotic progression is driven by the sequential translational activation of maternal messenger RNAs stored in the cytoplasm. This activation is mainly induced by the cytoplasmic elongation of their poly(A) tails, which is mediated by the cytoplasmic polyadenylation element (CPE) present in their 3’ untranslated regions (3´ UTRs). Sequential, phase-specific translation of these maternal mRNAs is required to complete the two meiotic divisions. Although the earlier polyadenylation events in prophase I and metaphase I are driven by the CPE-binding protein 1 (CPEB1), 90% of this protein is degraded by the anaphase promoting complex in the first meiotic division. The low levels of CPEB1 during interkinesis and in metaphase II raise the question of how the cytoplasmic polyadenylation required for the second meiotic division is achieved. In this work, we demonstrate that CPEB1 activates the translation of the maternal mRNA encoding CPEB4, which, in turn, recruits the cytoplasmic poly(A) polymerase GLD2 to “late” CPE-regulated mRNAs driving the transition from metaphase I to metaphase II, and, therefore, replacing CPEB1 for “late” meiosis polyadenylation.
publishDate 2009
dc.date.none.fl_str_mv 2009
2011
2011
dc.type.none.fl_str_mv info:eu-repo/semantics/doctoralThesis
info:eu-repo/semantics/publishedVersion
format doctoralThesis
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10803/22687
url http://hdl.handle.net/10803/22687
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 224 p.
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universitat Pompeu Fabra
publisher.none.fl_str_mv Universitat Pompeu Fabra
dc.source.none.fl_str_mv TDX (Tesis Doctorals en Xarxa)
reponame:TDR. Tesis Doctorales en Red
instname:CBUC, CESCA
instname_str CBUC, CESCA
reponame_str TDR. Tesis Doctorales en Red
collection TDR. Tesis Doctorales en Red
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869408336706797568
score 15.301629

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