Genetic changes including gene copy number alterations and their relation to prognosis in childhood acute myeloid leukemia

We studied a series of 68 subjects diagnosed with childhood acute myeloid leukemia (AML) using conventional cytogenetics and fluorescence in situ hybridization (FISH), polymerase chain reaction (PCR) to analyze mutations in FLT3 and NPM1 genes, and/or array comparative genomic hybridization (CGH). C...

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Detalhes bibliográficos
Autores: Armengol, Gemma|||0000-0003-2345-1106, Canellas, Anna, Álvarez, Yolanda, Bastida, Pilar, Sánchez de Toledo Codina, José|||0000-0002-1034-1920, Pérez-Iribarne, Maria Del Mar, Camós, Mireia|||0000-0003-3658-7942, Tuset, Esperanza|||0000-0002-1463-6689, Estella, Jesús, Coll, María Dolores, Caballín, María Rosa|||0000-0002-0091-5484, Knuutila, Sakari
Formato: artículo
Fecha de publicación:2010
País:España
Recursos:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:320848
Acesso em linha:https://ddd.uab.cat/record/320848
https://dx.doi.org/urn:doi:10.3109/10428190903350397
Access Level:acceso abierto
Palavra-chave:Array CGH
Childhood AML
Cytogenetics
Genetic mutations
Genomic imbalances
Prognosis
Descrição
Resumo:We studied a series of 68 subjects diagnosed with childhood acute myeloid leukemia (AML) using conventional cytogenetics and fluorescence in situ hybridization (FISH), polymerase chain reaction (PCR) to analyze mutations in FLT3 and NPM1 genes, and/or array comparative genomic hybridization (CGH). Cytogenetic/FISH abnormalities were observed in 71% of subjects, FLT3-ITD mutations in 15%, and NPM1 mutations in 13%. The array CGH alterations (average 3.6 per case) were observed in 96% of the tested subjects. The most frequent alterations were gains of 8q24.3 and 11p15.5-p15.4 in 16% of the samples. Six genes (AKT1, RUNX1, LTB, SDC1, RUNX1T1, and JAK2) from the imbalanced regions have been reported to be involved in AML, whereas other 30 cancer genes, not previously reported in an AML context, were identified as imbalanced. They probably correspond to non passenger alterations that cooperate with the recurrent translocations. The clinical data and genetic changes were tested to find out the possible association with prognosis. Genomic instability (four or more genomic imbalances) was correlated with poor patient outcome (p=0.029).

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