Interaction of the attenuated recombinant rIHNV-Gvhsv GFP virus with macrophages from rainbow trout (Oncorhynchus mykiss)

11 pages, 7 figures

Detalles Bibliográficos
Autores: Romero Jódar, Alejandro, Dios, S., Bremont, Michel, Figueras Huerta, Antonio, Novoa, Beatriz
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2011
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/47718
Acceso en línea:http://hdl.handle.net/10261/47718
Access Level:acceso abierto
Palabra clave:RIHNV-Gvhsv GFP
IHNV
Rhabdovirus
Macrophages
TEM
ROS
Apoptosis
Trout
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spelling Interaction of the attenuated recombinant rIHNV-Gvhsv GFP virus with macrophages from rainbow trout (Oncorhynchus mykiss)Romero Jódar, AlejandroDios, S.Bremont, MichelFigueras Huerta, AntonioNovoa, BeatrizRIHNV-Gvhsv GFPIHNVRhabdovirusMacrophagesTEMROSApoptosisTrout11 pages, 7 figuresOne of the most important threats to the salmonid aquaculture industry is infection caused by novirhabdoviruses such as infectious haematopoietic necrosis virus (IHNV) or viral haemorrhagic septicaemia virus (VHSV). Using reverse genetics, an avirulent recombinant rIHNV-Gvhsv GFP strain was generated, which was able to replicate as effectively as wild type IHNV in a fish cell line and in macrophages. Although this recombinant virus induced protective responses against IHNV and VHSV, the response did not involve the production of antibodies or modulate the expression of some antiviral genes. To determine the immune mechanisms underlying the protection conferred by the rIHNV-Gvhsv GFP virus, different immune parameters (NO production, respiratory burst activity and the induction of apoptosis) were assessed in the macrophage population. The results obtained in the present work may indicate that the Nv protein could be important in the modulation of NO and ROS production. rIHNV-Gvhsv GFP did not appear to have a clear effect on nitric oxide production or apoptosis. However, an increased respiratory burst activity (with levels induced by the recombinant virus significantly higher than the levels induced by the wild type virus), suggests a stimulation of the macrophage population, which could be related to the protection against virulent viruses.This work was supported by the projects FAIRCT 98-4398 from the European Union and BIO 2000-0906 from the Spanish Ministerio de Ciencia y Tecnología. European structural funds were used for confocal microscopy. Fondo Europeo de Desarrollo Regional (FEDER) y Ministerio de Ciencia e Innovación. A. Romero acknowledges the CSIC for the I3P fellowship.Peer reviewedElsevierEuropean CommissionMinisterio de Ciencia y Tecnología (España)Ministerio de Ciencia e Innovación (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201220122011info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/47718reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1016/j.vetimm.2010.12.001Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/477182026-05-22T06:33:51Z
dc.title.none.fl_str_mv Interaction of the attenuated recombinant rIHNV-Gvhsv GFP virus with macrophages from rainbow trout (Oncorhynchus mykiss)
title Interaction of the attenuated recombinant rIHNV-Gvhsv GFP virus with macrophages from rainbow trout (Oncorhynchus mykiss)
spellingShingle Interaction of the attenuated recombinant rIHNV-Gvhsv GFP virus with macrophages from rainbow trout (Oncorhynchus mykiss)
Romero Jódar, Alejandro
RIHNV-Gvhsv GFP
IHNV
Rhabdovirus
Macrophages
TEM
ROS
Apoptosis
Trout
title_short Interaction of the attenuated recombinant rIHNV-Gvhsv GFP virus with macrophages from rainbow trout (Oncorhynchus mykiss)
title_full Interaction of the attenuated recombinant rIHNV-Gvhsv GFP virus with macrophages from rainbow trout (Oncorhynchus mykiss)
title_fullStr Interaction of the attenuated recombinant rIHNV-Gvhsv GFP virus with macrophages from rainbow trout (Oncorhynchus mykiss)
title_full_unstemmed Interaction of the attenuated recombinant rIHNV-Gvhsv GFP virus with macrophages from rainbow trout (Oncorhynchus mykiss)
title_sort Interaction of the attenuated recombinant rIHNV-Gvhsv GFP virus with macrophages from rainbow trout (Oncorhynchus mykiss)
dc.creator.none.fl_str_mv Romero Jódar, Alejandro
Dios, S.
Bremont, Michel
Figueras Huerta, Antonio
Novoa, Beatriz
author Romero Jódar, Alejandro
author_facet Romero Jódar, Alejandro
Dios, S.
Bremont, Michel
Figueras Huerta, Antonio
Novoa, Beatriz
author_role author
author2 Dios, S.
Bremont, Michel
Figueras Huerta, Antonio
Novoa, Beatriz
author2_role author
author
author
author
dc.contributor.none.fl_str_mv European Commission
Ministerio de Ciencia y Tecnología (España)
Ministerio de Ciencia e Innovación (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv RIHNV-Gvhsv GFP
IHNV
Rhabdovirus
Macrophages
TEM
ROS
Apoptosis
Trout
topic RIHNV-Gvhsv GFP
IHNV
Rhabdovirus
Macrophages
TEM
ROS
Apoptosis
Trout
description 11 pages, 7 figures
publishDate 2011
dc.date.none.fl_str_mv 2011
2012
2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/47718
url http://hdl.handle.net/10261/47718
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1016/j.vetimm.2010.12.001
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869408326079479808
score 15,81155

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