903 Protein Saver cards: the best alternative for dried blood spot storage at room temperature for HCV RNA

Hepatitis C virus (HCV) infection remains a global health problem, detected only in the early stages by molecular tests. Molecular tests detect HCV RNA, which is very prone to degradation by ribonucleases, reason why blood samples must be transported and stored at − 20 °C, or even − 70 °C for long-t...

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Detalles Bibliográficos
Autores: Madrid González, Ricardo, Arca‑Lafuente, Sonia, Casanueva‑Benítez, Cristina, Crespo‑Bermejo, Celia, Lara‑Aguilar, Violeta, Martín‑Carbonero, Luz, Santos, Ignacio de los, Briz, Verónica
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/72532
Acceso en línea:https://hdl.handle.net/20.500.14352/72532
Access Level:acceso abierto
Palabra clave:578.89
578.5
Hepatitis C virus (HCV)
RNA Virus
903 Protein
Genética
Microbiología (Biología)
2409 Genética
2414 Microbiología
id ES_55a3e111a871216f76e56d0e4b8a3d1e
oai_identifier_str oai:docta.ucm.es:20.500.14352/72532
network_acronym_str ES
network_name_str España
repository_id_str
spelling 903 Protein Saver cards: the best alternative for dried blood spot storage at room temperature for HCV RNAMadrid González, RicardoArca‑Lafuente, SoniaCasanueva‑Benítez, CristinaCrespo‑Bermejo, CeliaLara‑Aguilar, VioletaMartín‑Carbonero, LuzSantos, Ignacio de losBriz, Verónica578.89578.5Hepatitis C virus (HCV)RNA Virus903 ProteinGenéticaMicrobiología (Biología)2409 Genética2414 MicrobiologíaHepatitis C virus (HCV) infection remains a global health problem, detected only in the early stages by molecular tests. Molecular tests detect HCV RNA, which is very prone to degradation by ribonucleases, reason why blood samples must be transported and stored at − 20 °C, or even − 70 °C for long-term storage. Flinders Technology Associates (FTA) cards are a useful sampling collecting device for dry blood spot (DBS) storage, especially for low and middle-income countries (LMIC). In this study, we analyzed viral HCV RNA integrity for long-term storage at room temperature compared to − 20 °C using two diferent types of cards for DBS: FTA Classic and 903 Protein Saver cards. For this purpose, DBS were prepared on these cards using blood or plasma samples from HCV infected patients, and samples were analysed by conventional RT-PCR. Our results showed that 903 Protein Saver cards are the best and cheapest alternative for DBS storage at room temperature. In these conditions, we found that HCV RNA integrity lasted for up to 9 months.Nature ResearchUniversidad Complutense de Madrid20222022-06-1620222022-06-16journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/72532reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/725322026-06-02T12:44:21Z
dc.title.none.fl_str_mv 903 Protein Saver cards: the best alternative for dried blood spot storage at room temperature for HCV RNA
title 903 Protein Saver cards: the best alternative for dried blood spot storage at room temperature for HCV RNA
spellingShingle 903 Protein Saver cards: the best alternative for dried blood spot storage at room temperature for HCV RNA
Madrid González, Ricardo
578.89
578.5
Hepatitis C virus (HCV)
RNA Virus
903 Protein
Genética
Microbiología (Biología)
2409 Genética
2414 Microbiología
title_short 903 Protein Saver cards: the best alternative for dried blood spot storage at room temperature for HCV RNA
title_full 903 Protein Saver cards: the best alternative for dried blood spot storage at room temperature for HCV RNA
title_fullStr 903 Protein Saver cards: the best alternative for dried blood spot storage at room temperature for HCV RNA
title_full_unstemmed 903 Protein Saver cards: the best alternative for dried blood spot storage at room temperature for HCV RNA
title_sort 903 Protein Saver cards: the best alternative for dried blood spot storage at room temperature for HCV RNA
dc.creator.none.fl_str_mv Madrid González, Ricardo
Arca‑Lafuente, Sonia
Casanueva‑Benítez, Cristina
Crespo‑Bermejo, Celia
Lara‑Aguilar, Violeta
Martín‑Carbonero, Luz
Santos, Ignacio de los
Briz, Verónica
author Madrid González, Ricardo
author_facet Madrid González, Ricardo
Arca‑Lafuente, Sonia
Casanueva‑Benítez, Cristina
Crespo‑Bermejo, Celia
Lara‑Aguilar, Violeta
Martín‑Carbonero, Luz
Santos, Ignacio de los
Briz, Verónica
author_role author
author2 Arca‑Lafuente, Sonia
Casanueva‑Benítez, Cristina
Crespo‑Bermejo, Celia
Lara‑Aguilar, Violeta
Martín‑Carbonero, Luz
Santos, Ignacio de los
Briz, Verónica
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 578.89
578.5
Hepatitis C virus (HCV)
RNA Virus
903 Protein
Genética
Microbiología (Biología)
2409 Genética
2414 Microbiología
topic 578.89
578.5
Hepatitis C virus (HCV)
RNA Virus
903 Protein
Genética
Microbiología (Biología)
2409 Genética
2414 Microbiología
description Hepatitis C virus (HCV) infection remains a global health problem, detected only in the early stages by molecular tests. Molecular tests detect HCV RNA, which is very prone to degradation by ribonucleases, reason why blood samples must be transported and stored at − 20 °C, or even − 70 °C for long-term storage. Flinders Technology Associates (FTA) cards are a useful sampling collecting device for dry blood spot (DBS) storage, especially for low and middle-income countries (LMIC). In this study, we analyzed viral HCV RNA integrity for long-term storage at room temperature compared to − 20 °C using two diferent types of cards for DBS: FTA Classic and 903 Protein Saver cards. For this purpose, DBS were prepared on these cards using blood or plasma samples from HCV infected patients, and samples were analysed by conventional RT-PCR. Our results showed that 903 Protein Saver cards are the best and cheapest alternative for DBS storage at room temperature. In these conditions, we found that HCV RNA integrity lasted for up to 9 months.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-06-16
2022
2022-06-16
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/72532
url https://hdl.handle.net/20.500.14352/72532
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Nature Research
publisher.none.fl_str_mv Nature Research
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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