Shifts in carbon substrate utilization in sediment microbial communities along the Llobregat River

River sediment microorganisms have the capacity to metabolize, uptake and decompose flowing water organic materials, which strongly depend on their metabolic capabilities. The changes of microbial functional diversity in river sediments were analysed at five sites along the River Llobregat (NE Spain...

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Detalles Bibliográficos
Autores: Freixa Casals, Anna, Romaní i Cornet, Anna M.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10256/11545
Acceso en línea:http://hdl.handle.net/10256/11545
Access Level:acceso embargado
Palabra clave:Ecologia microbiana
Microbial ecology
Microorganismes -- Catalunya -- Llobregat (curs d'aigua)
Microorganisms -- Catalonia -- Llobregat (River)
Sediments fluvials -- Catalunya -- Llobregat (curs d'aigua)
River sediments -- Catalonia -- Llobregat (River)
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spelling Shifts in carbon substrate utilization in sediment microbial communities along the Llobregat RiverFreixa Casals, AnnaRomaní i Cornet, Anna M.Ecologia microbianaMicrobial ecologyMicroorganismes -- Catalunya -- Llobregat (curs d'aigua)Microorganisms -- Catalonia -- Llobregat (River)Sediments fluvials -- Catalunya -- Llobregat (curs d'aigua)River sediments -- Catalonia -- Llobregat (River)River sediment microorganisms have the capacity to metabolize, uptake and decompose flowing water organic materials, which strongly depend on their metabolic capabilities. The changes of microbial functional diversity in river sediments were analysed at five sites along the River Llobregat (NE Spain), using the Biolog EcoPlates incubation method. In parallel, we tested the potential application of the EcoPlates with preserved frozen samples (-80 °C). Although functional diversity (Shannon index) did not show significant differences, the specific carbon substrates that were used differed between sampling sites. The microbial community from the upstream site was the most active as shown by the highest respiratory activity and abundance of living bacteria, being able to metabolize more labile substrates. Downstream sediment communities showed a decrease in bacterial viability, respiration activity and EPS content, suggesting a less structured biofilm. The functional fingerprint analyses clearly distinguish the sediment microbial communities from the downstream most polluted sites, which showed a potential capacity to use more complex carbon substrates such as polymers. Freezing sediment samples is not recommended, since a significant reduction in the functional diversity and changes in the fingerprint patterns were observed together with a reduction of the number of substrates utilisation especially at two downstream sites. The Biolog EcoPlates appear to be useful for detecting changes in the microbial functional fingerprint in fresh sediments along a river pollution gradientThis study was funded by the Spanish Ministry of Economy and Competitiveness projects FLUMED-HOTSPOTS (CGL2011-30151-C02-01) and SCARCE (Consolider-Ingenio CSD2009-00065)Schweizerbart Science PublishersMinisterio de Ciencia e Innovación (Espanya)infoinfo2014info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10256/11545http://hdl.handle.net/10256/11545© Fundamental and Applied Limnology, 2014, vol. 185, núms. 3-4, p. 247-261Articles publicats (D-CCAA)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/semantics/altIdentifier/doi/10.1127/fal/2014/0588info:eu-repo/semantics/altIdentifier/issn/1863-9135info:eu-repo/semantics/altIdentifier/eissn/2363-7110info:eu-repo/grantAgreement/MICINN//CGL2011-30151-C02-01info:eu-repo/grantAgreement/MICINN//CSD2009-00065Tots els drets reservatsinfo:eu-repo/semantics/embargoedAccessoai:recercat.cat:10256/115452026-05-29T05:05:01Z
dc.title.none.fl_str_mv Shifts in carbon substrate utilization in sediment microbial communities along the Llobregat River
title Shifts in carbon substrate utilization in sediment microbial communities along the Llobregat River
spellingShingle Shifts in carbon substrate utilization in sediment microbial communities along the Llobregat River
Freixa Casals, Anna
Ecologia microbiana
Microbial ecology
Microorganismes -- Catalunya -- Llobregat (curs d'aigua)
Microorganisms -- Catalonia -- Llobregat (River)
Sediments fluvials -- Catalunya -- Llobregat (curs d'aigua)
River sediments -- Catalonia -- Llobregat (River)
title_short Shifts in carbon substrate utilization in sediment microbial communities along the Llobregat River
title_full Shifts in carbon substrate utilization in sediment microbial communities along the Llobregat River
title_fullStr Shifts in carbon substrate utilization in sediment microbial communities along the Llobregat River
title_full_unstemmed Shifts in carbon substrate utilization in sediment microbial communities along the Llobregat River
title_sort Shifts in carbon substrate utilization in sediment microbial communities along the Llobregat River
dc.creator.none.fl_str_mv Freixa Casals, Anna
Romaní i Cornet, Anna M.
author Freixa Casals, Anna
author_facet Freixa Casals, Anna
Romaní i Cornet, Anna M.
author_role author
author2 Romaní i Cornet, Anna M.
author2_role author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (Espanya)
dc.subject.none.fl_str_mv Ecologia microbiana
Microbial ecology
Microorganismes -- Catalunya -- Llobregat (curs d'aigua)
Microorganisms -- Catalonia -- Llobregat (River)
Sediments fluvials -- Catalunya -- Llobregat (curs d'aigua)
River sediments -- Catalonia -- Llobregat (River)
topic Ecologia microbiana
Microbial ecology
Microorganismes -- Catalunya -- Llobregat (curs d'aigua)
Microorganisms -- Catalonia -- Llobregat (River)
Sediments fluvials -- Catalunya -- Llobregat (curs d'aigua)
River sediments -- Catalonia -- Llobregat (River)
description River sediment microorganisms have the capacity to metabolize, uptake and decompose flowing water organic materials, which strongly depend on their metabolic capabilities. The changes of microbial functional diversity in river sediments were analysed at five sites along the River Llobregat (NE Spain), using the Biolog EcoPlates incubation method. In parallel, we tested the potential application of the EcoPlates with preserved frozen samples (-80 °C). Although functional diversity (Shannon index) did not show significant differences, the specific carbon substrates that were used differed between sampling sites. The microbial community from the upstream site was the most active as shown by the highest respiratory activity and abundance of living bacteria, being able to metabolize more labile substrates. Downstream sediment communities showed a decrease in bacterial viability, respiration activity and EPS content, suggesting a less structured biofilm. The functional fingerprint analyses clearly distinguish the sediment microbial communities from the downstream most polluted sites, which showed a potential capacity to use more complex carbon substrates such as polymers. Freezing sediment samples is not recommended, since a significant reduction in the functional diversity and changes in the fingerprint patterns were observed together with a reduction of the number of substrates utilisation especially at two downstream sites. The Biolog EcoPlates appear to be useful for detecting changes in the microbial functional fingerprint in fresh sediments along a river pollution gradient
publishDate 2014
dc.date.none.fl_str_mv 2014
info
info
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10256/11545
http://hdl.handle.net/10256/11545
url http://hdl.handle.net/10256/11545
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1127/fal/2014/0588
info:eu-repo/semantics/altIdentifier/issn/1863-9135
info:eu-repo/semantics/altIdentifier/eissn/2363-7110
info:eu-repo/grantAgreement/MICINN//CGL2011-30151-C02-01
info:eu-repo/grantAgreement/MICINN//CSD2009-00065
dc.rights.none.fl_str_mv Tots els drets reservats
info:eu-repo/semantics/embargoedAccess
rights_invalid_str_mv Tots els drets reservats
eu_rights_str_mv embargoedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Schweizerbart Science Publishers
publisher.none.fl_str_mv Schweizerbart Science Publishers
dc.source.none.fl_str_mv © Fundamental and Applied Limnology, 2014, vol. 185, núms. 3-4, p. 247-261
Articles publicats (D-CCAA)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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