Characterization of two transcriptional activators: MafR of Enterococcus faecalis and MgaP of Streptococcus pneumoniae
The Gram-positive bacteria Enterococcus faecalis and Streptococcus pneumoniae are inhabitants of the human body. Whereas E. faecalis is a usual commensal of the gastrointestinal tract, S. pneumoniae is normally found in the nasopharynx of healthy individuals. Nevertheless, their ability to adapt to...
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| Tipo de recurso: | tesis doctoral |
| Fecha de publicación: | 2021 |
| País: | España |
| Institución: | Universidad Complutense de Madrid (UCM) |
| Repositorio: | Docta Complutense |
| Idioma: | inglés |
| OAI Identifier: | oai:docta.ucm.es:20.500.14352/11564 |
| Acceso en línea: | https://hdl.handle.net/20.500.14352/11564 |
| Access Level: | acceso abierto |
| Palabra clave: | 579.86(043.2) Transcriptional activators MafR of Enterococcus faecalis MgaP of Streptococcus pneumoniae Activadores transcripcionales MafR de Enterococcus faecalis MgaP de Streptococcus pneumoniae Microbiología (Biología) 2414 Microbiología |
| Sumario: | The Gram-positive bacteria Enterococcus faecalis and Streptococcus pneumoniae are inhabitants of the human body. Whereas E. faecalis is a usual commensal of the gastrointestinal tract, S. pneumoniae is normally found in the nasopharynx of healthy individuals. Nevertheless, their ability to adapt to diverse stress conditions allows them to invade other niches of the human host causing a variety of life-threatening infections. In general, bacterial adaptation to new niches requires the action of proteins that act as global transcriptional regulators. These proteins activate and/or repress the transcription of multiple genes in response to specific signals. The Mga/AtxA family of global transcriptional regulators includes various proteins of Gram-positive pathogenic bacteria: AtxA (Bacillus anthracis), Mga (S. pyogenes), MgaSpn (S. pneumoniae), and MafR (E. faecalis). Several studies have shown that these transcriptional regulators play a role in bacterial virulence (Fouet, 2010; Hemsley et al., 2003; McIver, 2009; Ruiz-Cruz et al., 2016). MafR from Enterococcus faecalis: Previous studies in our laboratory showed that the MafR protein of E. faecalis strain V583 (MafRV583) binds to linear DNA fragments generating multimeric complexes, and this binding has little or no sequence specificity (Ruiz-Cruz, 2015). Furthermore, using microarrays designed for strain OG1RF, it was shown that MafR activates, directly or indirectly, the transcription of at least 87 genes. Many of them are organized in operons and encode proteins involved in the utilization of carbon sources (Ruiz-Cruz et al., 2016). In this Thesis, we have worked on the following objectives: (a) Analysis of the DNA-binding properties of MafR from strain OG1RF (MafROG1RF) and (b) Identification of genes regulated directly by MafROG1RF... |
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