Proteomic profiling of extracellular vesicles in inflammatory bowel diseases
The proteomic analysis of serum extracellular vesicles (EVs) could be a useful tool for studying the pathophysiology of Crohn’s disease (CD) and ulcerative colitis (UC), as well as for biomarker discovery. To characterize the proteomic composition of serum EVs in patients with CD and UC to identify...
| Autores: | , , , , , , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2025 |
| País: | España |
| Institución: | Universidad del País Vasco |
| Repositorio: | Addi. Archivo Digital para la Docencia y la Investigación |
| OAI Identifier: | oai:addi.ehu.eus:10810/72610 |
| Acceso en línea: | http://hdl.handle.net/10810/72610 |
| Access Level: | acceso abierto |
| Palabra clave: | Crohn’s disease ulcerative colitis extracellular vesicles proteomics |
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Proteomic profiling of extracellular vesicles in inflammatory bowel diseasesBaldán Martín, MontserratAzkargorta, MikelLapitz Dambolenea, AinhoaOrtega Moreno, AinhoaLloro, IbónFernández Tomé, SamuelArbelaiz Cossio, AnderEscobes, IraideMarín, Alicia C.Bernardo, DavidBujanda Fernández de Pierola, LuisBañales Asurmendi, Jesús MaríaElortza, FelixGisbert, Javier P.Chaparro, MaríaCrohn’s diseaseulcerative colitisextracellular vesiclesproteomicsThe proteomic analysis of serum extracellular vesicles (EVs) could be a useful tool for studying the pathophysiology of Crohn’s disease (CD) and ulcerative colitis (UC), as well as for biomarker discovery. To characterize the proteomic composition of serum EVs in patients with CD and UC to identify biomarkers and molecular pathways associated with pathogenesis and activity. Methods: Serum EVs were enriched and analyzed in patients with quiescent CD, active CD (aCD), quiescent UC, active UC (aUC), and healthy controls (HCs) (n = 30 per group). All groups were matched for age and sex. Disease activity was assessed by ileocolonoscopy and categorized based on the SES-CD (CD) and the endoscopic sub-score of the Mayo Score (UC). EVs were enriched by ul-tracentrifugation, and their size and concentration were determined by nanoparticle tracking analysis. The expression of CD63, CD81, and CD9 was determined using West-ern blotting. Proteomic analysis was performed by label-free nano-LC MS/MS. A total of 324 proteins were identified; 60 showed differential abundance in CD-HC, 34 in UC-HC, and 21 in CD-UC. Regarding disease activity, the abundance of 58 and 32 proteins was altered in aCD-HC and aUC-HC, respectively. Functional analyses revealed that proteins associated with aCD were involved in immune regulation, whereas those linked to aUC were enriched in oxidative stress. We have identified expressed proteins between EVs from patients with CD and UC, depending on the presence of disease, the disease type, and the disease activity. These proteins are potential candidates as disease biomarkers and open new research avenues to better understand these conditions.This research has been funded by grants from the Spanish Working Group on Crohn’s Disease and Ulcerative Colitis (GETECCU), XX (Beca GETECCU-Faes Farma), European Crohn’s and Colitis Organisation (ECCO), and Asociación Española de Gastroenterología (Beca Gonzalo Miño). D.B. is also a member of the CSIC’s Global Health Platform (PTI Salud Global).MDPI202520252025info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10810/72610reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoIngléshttps://www.mdpi.com/1422-0067/26/2/526info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/© 2025 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).oai:addi.ehu.eus:10810/726102026-06-18T09:23:17Z |
| dc.title.none.fl_str_mv |
Proteomic profiling of extracellular vesicles in inflammatory bowel diseases |
| title |
Proteomic profiling of extracellular vesicles in inflammatory bowel diseases |
| spellingShingle |
Proteomic profiling of extracellular vesicles in inflammatory bowel diseases Baldán Martín, Montserrat Crohn’s disease ulcerative colitis extracellular vesicles proteomics |
| title_short |
Proteomic profiling of extracellular vesicles in inflammatory bowel diseases |
| title_full |
Proteomic profiling of extracellular vesicles in inflammatory bowel diseases |
| title_fullStr |
Proteomic profiling of extracellular vesicles in inflammatory bowel diseases |
| title_full_unstemmed |
Proteomic profiling of extracellular vesicles in inflammatory bowel diseases |
| title_sort |
Proteomic profiling of extracellular vesicles in inflammatory bowel diseases |
| dc.creator.none.fl_str_mv |
Baldán Martín, Montserrat Azkargorta, Mikel Lapitz Dambolenea, Ainhoa Ortega Moreno, Ainhoa Lloro, Ibón Fernández Tomé, Samuel Arbelaiz Cossio, Ander Escobes, Iraide Marín, Alicia C. Bernardo, David Bujanda Fernández de Pierola, Luis Bañales Asurmendi, Jesús María Elortza, Felix Gisbert, Javier P. Chaparro, María |
| author |
Baldán Martín, Montserrat |
| author_facet |
Baldán Martín, Montserrat Azkargorta, Mikel Lapitz Dambolenea, Ainhoa Ortega Moreno, Ainhoa Lloro, Ibón Fernández Tomé, Samuel Arbelaiz Cossio, Ander Escobes, Iraide Marín, Alicia C. Bernardo, David Bujanda Fernández de Pierola, Luis Bañales Asurmendi, Jesús María Elortza, Felix Gisbert, Javier P. Chaparro, María |
| author_role |
author |
| author2 |
Azkargorta, Mikel Lapitz Dambolenea, Ainhoa Ortega Moreno, Ainhoa Lloro, Ibón Fernández Tomé, Samuel Arbelaiz Cossio, Ander Escobes, Iraide Marín, Alicia C. Bernardo, David Bujanda Fernández de Pierola, Luis Bañales Asurmendi, Jesús María Elortza, Felix Gisbert, Javier P. Chaparro, María |
| author2_role |
author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Crohn’s disease ulcerative colitis extracellular vesicles proteomics |
| topic |
Crohn’s disease ulcerative colitis extracellular vesicles proteomics |
| description |
The proteomic analysis of serum extracellular vesicles (EVs) could be a useful tool for studying the pathophysiology of Crohn’s disease (CD) and ulcerative colitis (UC), as well as for biomarker discovery. To characterize the proteomic composition of serum EVs in patients with CD and UC to identify biomarkers and molecular pathways associated with pathogenesis and activity. Methods: Serum EVs were enriched and analyzed in patients with quiescent CD, active CD (aCD), quiescent UC, active UC (aUC), and healthy controls (HCs) (n = 30 per group). All groups were matched for age and sex. Disease activity was assessed by ileocolonoscopy and categorized based on the SES-CD (CD) and the endoscopic sub-score of the Mayo Score (UC). EVs were enriched by ul-tracentrifugation, and their size and concentration were determined by nanoparticle tracking analysis. The expression of CD63, CD81, and CD9 was determined using West-ern blotting. Proteomic analysis was performed by label-free nano-LC MS/MS. A total of 324 proteins were identified; 60 showed differential abundance in CD-HC, 34 in UC-HC, and 21 in CD-UC. Regarding disease activity, the abundance of 58 and 32 proteins was altered in aCD-HC and aUC-HC, respectively. Functional analyses revealed that proteins associated with aCD were involved in immune regulation, whereas those linked to aUC were enriched in oxidative stress. We have identified expressed proteins between EVs from patients with CD and UC, depending on the presence of disease, the disease type, and the disease activity. These proteins are potential candidates as disease biomarkers and open new research avenues to better understand these conditions. |
| publishDate |
2025 |
| dc.date.none.fl_str_mv |
2025 2025 2025 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article |
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article |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10810/72610 |
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http://hdl.handle.net/10810/72610 |
| dc.language.none.fl_str_mv |
Inglés |
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Inglés |
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https://www.mdpi.com/1422-0067/26/2/526 |
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info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ |
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openAccess |
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http://creativecommons.org/licenses/by/4.0/ |
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application/pdf |
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MDPI |
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MDPI |
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reponame:Addi. Archivo Digital para la Docencia y la Investigación instname:Universidad del País Vasco |
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Universidad del País Vasco |
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Addi. Archivo Digital para la Docencia y la Investigación |
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Addi. Archivo Digital para la Docencia y la Investigación |
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