Autocrine regulation of human prostate carcinoma cell proliferation by somatostatin through the modulation of the SH2 domain containing protein tyrosine phosphatase (SHP)-1

The present study was intended to gain additional information on the growth regulation of prostate by somatostatin (SRIF) and the intracellular events involved. The humanprostate adenocarcinoma cell lines PC-3 and LNCaP produce SRIF and express subtypes 2 and 5 of SRIF receptors. The secretion of SR...

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Autores: Zapata, Pedro Darío, Ropero, Rosa María, Valencia Torralba, Ana M., Buscail, Louis, López, José Ignacio, Martín Orozco, Rosa María, Prieto Villapún, Juan Carlos, Angulo Cuesta, Javier, Susini, Christiane, López Ruiz, María del Pilar, Colás Escudero, María Begoña|||0000-0001-7913-3006
Tipo de recurso: artículo
Fecha de publicación:2002
País:España
Institución:Universidad de Alcalá (UAH)
Repositorio:e_Buah Biblioteca Digital Universidad de Alcalá
Idioma:inglés
OAI Identifier:oai:ebuah.uah.es:10017/7987
Acceso en línea:http://hdl.handle.net/10017/7987
https://dx.doi.org/10.1210/jcem.87.2.8194
Access Level:acceso abierto
Palabra clave:Somatostatin
SRIF
Human prostate carcinoma
Tyrosine phosphatase
Bioquímica
Ciencia
Biochemistry
Science
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spelling Autocrine regulation of human prostate carcinoma cell proliferation by somatostatin through the modulation of the SH2 domain containing protein tyrosine phosphatase (SHP)-1Zapata, Pedro DaríoRopero, Rosa MaríaValencia Torralba, Ana M.Buscail, LouisLópez, José IgnacioMartín Orozco, Rosa MaríaPrieto Villapún, Juan CarlosAngulo Cuesta, JavierSusini, ChristianeLópez Ruiz, María del PilarColás Escudero, María Begoña|||0000-0001-7913-3006SomatostatinSRIFHuman prostate carcinomaTyrosine phosphataseBioquímicaCienciaBiochemistryScienceThe present study was intended to gain additional information on the growth regulation of prostate by somatostatin (SRIF) and the intracellular events involved. The humanprostate adenocarcinoma cell lines PC-3 and LNCaP produce SRIF and express subtypes 2 and 5 of SRIF receptors. The secretion of SRIF is related to the proliferative status of these cells; an inverse relationship exists between cell proliferation and the amount of secreted SRIF. Moreover, the growth of PC-3 cells is inhibited by SRIF overexpression and increased by blockage of endogenous SRIF. Coincident with the increase in SRIF secretion, the activity and levels of theSH2domain containing protein tyrosine phosphatase (SHP)-1, present in PC-3 cells are augmented, but the effect can be partially prevented by neutralization of secreted endogenously SRIF. The activity of SHP-1 is also stimulated by the SRIF analog RC160. Overexpression of SHP-1 induces inhibition of PC-3 cell growth. SHP-1 is also present in normal prostate, benign prostatic hyperplasia, prostatic intraepithelial neoplasia, and well differentiated adenocarcinoma. In contrast, no signal is detected in poorly differentiated prostate cancer. These findings demonstrate that SRIF inhibits PC-3 and LNCaP cell proliferation through an autocrine/paracrine SRIF loop. This effect could be mediated by activation of the tyrosine phosphatase SHP-1 detected in these cells as well as in human prostate and prostate cancer.Comisión Asesora de Investigación Científica y TécnicaMinisterio de Educación y CienciaEndocrine Society20022002-01-01journal articlehttp://purl.org/coar/resource_type/c_6501NAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10017/7987https://dx.doi.org/10.1210/jcem.87.2.8194reponame:e_Buah Biblioteca Digital Universidad de Alcaláinstname:Universidad de Alcalá (UAH)InglésengCAICYT Not available PM97–0069 Mecanismos de transducción de señales en la proliferación y apoptosis celular en próstata normal y en carcinoma e hiperplasia benigna de próstataMinisterio de Educación y Ciencia Not available HF-1998-0132open accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:ebuah.uah.es:10017/79872026-06-18T11:13:07Z
dc.title.none.fl_str_mv Autocrine regulation of human prostate carcinoma cell proliferation by somatostatin through the modulation of the SH2 domain containing protein tyrosine phosphatase (SHP)-1
title Autocrine regulation of human prostate carcinoma cell proliferation by somatostatin through the modulation of the SH2 domain containing protein tyrosine phosphatase (SHP)-1
spellingShingle Autocrine regulation of human prostate carcinoma cell proliferation by somatostatin through the modulation of the SH2 domain containing protein tyrosine phosphatase (SHP)-1
Zapata, Pedro Darío
Somatostatin
SRIF
Human prostate carcinoma
Tyrosine phosphatase
Bioquímica
Ciencia
Biochemistry
Science
title_short Autocrine regulation of human prostate carcinoma cell proliferation by somatostatin through the modulation of the SH2 domain containing protein tyrosine phosphatase (SHP)-1
title_full Autocrine regulation of human prostate carcinoma cell proliferation by somatostatin through the modulation of the SH2 domain containing protein tyrosine phosphatase (SHP)-1
title_fullStr Autocrine regulation of human prostate carcinoma cell proliferation by somatostatin through the modulation of the SH2 domain containing protein tyrosine phosphatase (SHP)-1
title_full_unstemmed Autocrine regulation of human prostate carcinoma cell proliferation by somatostatin through the modulation of the SH2 domain containing protein tyrosine phosphatase (SHP)-1
title_sort Autocrine regulation of human prostate carcinoma cell proliferation by somatostatin through the modulation of the SH2 domain containing protein tyrosine phosphatase (SHP)-1
dc.creator.none.fl_str_mv Zapata, Pedro Darío
Ropero, Rosa María
Valencia Torralba, Ana M.
Buscail, Louis
López, José Ignacio
Martín Orozco, Rosa María
Prieto Villapún, Juan Carlos
Angulo Cuesta, Javier
Susini, Christiane
López Ruiz, María del Pilar
Colás Escudero, María Begoña|||0000-0001-7913-3006
author Zapata, Pedro Darío
author_facet Zapata, Pedro Darío
Ropero, Rosa María
Valencia Torralba, Ana M.
Buscail, Louis
López, José Ignacio
Martín Orozco, Rosa María
Prieto Villapún, Juan Carlos
Angulo Cuesta, Javier
Susini, Christiane
López Ruiz, María del Pilar
Colás Escudero, María Begoña|||0000-0001-7913-3006
author_role author
author2 Ropero, Rosa María
Valencia Torralba, Ana M.
Buscail, Louis
López, José Ignacio
Martín Orozco, Rosa María
Prieto Villapún, Juan Carlos
Angulo Cuesta, Javier
Susini, Christiane
López Ruiz, María del Pilar
Colás Escudero, María Begoña|||0000-0001-7913-3006
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Somatostatin
SRIF
Human prostate carcinoma
Tyrosine phosphatase
Bioquímica
Ciencia
Biochemistry
Science
topic Somatostatin
SRIF
Human prostate carcinoma
Tyrosine phosphatase
Bioquímica
Ciencia
Biochemistry
Science
description The present study was intended to gain additional information on the growth regulation of prostate by somatostatin (SRIF) and the intracellular events involved. The humanprostate adenocarcinoma cell lines PC-3 and LNCaP produce SRIF and express subtypes 2 and 5 of SRIF receptors. The secretion of SRIF is related to the proliferative status of these cells; an inverse relationship exists between cell proliferation and the amount of secreted SRIF. Moreover, the growth of PC-3 cells is inhibited by SRIF overexpression and increased by blockage of endogenous SRIF. Coincident with the increase in SRIF secretion, the activity and levels of theSH2domain containing protein tyrosine phosphatase (SHP)-1, present in PC-3 cells are augmented, but the effect can be partially prevented by neutralization of secreted endogenously SRIF. The activity of SHP-1 is also stimulated by the SRIF analog RC160. Overexpression of SHP-1 induces inhibition of PC-3 cell growth. SHP-1 is also present in normal prostate, benign prostatic hyperplasia, prostatic intraepithelial neoplasia, and well differentiated adenocarcinoma. In contrast, no signal is detected in poorly differentiated prostate cancer. These findings demonstrate that SRIF inhibits PC-3 and LNCaP cell proliferation through an autocrine/paracrine SRIF loop. This effect could be mediated by activation of the tyrosine phosphatase SHP-1 detected in these cells as well as in human prostate and prostate cancer.
publishDate 2002
dc.date.none.fl_str_mv 2002
2002-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
NA
http://purl.org/coar/version/c_be7fb7dd8ff6fe43
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10017/7987
https://dx.doi.org/10.1210/jcem.87.2.8194
url http://hdl.handle.net/10017/7987
https://dx.doi.org/10.1210/jcem.87.2.8194
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv CAICYT Not available PM97–0069 Mecanismos de transducción de señales en la proliferación y apoptosis celular en próstata normal y en carcinoma e hiperplasia benigna de próstata
Ministerio de Educación y Ciencia Not available HF-1998-0132
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Endocrine Society
publisher.none.fl_str_mv Endocrine Society
dc.source.none.fl_str_mv reponame:e_Buah Biblioteca Digital Universidad de Alcalá
instname:Universidad de Alcalá (UAH)
instname_str Universidad de Alcalá (UAH)
reponame_str e_Buah Biblioteca Digital Universidad de Alcalá
collection e_Buah Biblioteca Digital Universidad de Alcalá
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.301603

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