A Bivalent Live-Attenuated Vaccine for the Prevention of Equine Influenza Virus

22 Pág.

Detalles Bibliográficos
Autores: Blanco-Lobo, Pilar, Rodriguez, Laura, Reedy, Stephanie, Oladunni, Fatai S., Nogales, Aitor, Murcia, Pablo R., Chambers, Thomas M., Martínez-Sobrido, Luis
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/362376
Acceso en línea:http://hdl.handle.net/10261/362376
https://api.elsevier.com/content/abstract/scopus_id/85073419500
Access Level:acceso abierto
Palabra clave:Equine influenza virus
Live-attenuated influenza vaccine
Master donor virus
Recombinant virus
Reverse genetics techniques
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spelling A Bivalent Live-Attenuated Vaccine for the Prevention of Equine Influenza VirusBlanco-Lobo, PilarRodriguez, LauraReedy, StephanieOladunni, Fatai S.Nogales, AitorMurcia, Pablo R.Chambers, Thomas M.Martínez-Sobrido, LuisEquine influenza virusLive-attenuated influenza vaccineMaster donor virusRecombinant virusReverse genetics techniques22 Pág.Vaccination remains the most effective approach for preventing and controlling equine influenza virus (EIV) in horses. However, the ongoing evolution of EIV has increased the genetic and antigenic differences between currently available vaccines and circulating strains, resulting in suboptimal vaccine efficacy. As recommended by the World Organization for Animal Health (OIE), the inclusion of representative strains from clade 1 and clade 2 Florida sublineages of EIV in vaccines may maximize the protection against presently circulating viral strains. In this study, we used reverse genetics technologies to generate a bivalent EIV live-attenuated influenza vaccine (LAIV). We combined our previously described clade 1 EIV LAIV A/equine/Ohio/2003 H3N8 (Ohio/03 LAIV) with a newly generated clade 2 EIV LAIV that contains the six internal genes of Ohio/03 LAIV and the HA and NA of A/equine/Richmond/1/2007 H3N8 (Rich/07 LAIV). The safety profile, immunogenicity, and protection efficacy of this bivalent EIV LAIV was tested in the natural host, horses. Vaccination of horses with the bivalent EIV LAIV, following a prime-boost regimen, was safe and able to confer protection against challenge with clade 1 (A/equine/Kentucky/2014 H3N8) and clade 2 (A/equine/Richmond/2007) wild-type (WT) EIVs, as evidenced by a reduction of clinical signs, fever, and virus excretion. This is the first description of a bivalent LAIV for the prevention of EIV in horses that follows OIE recommendations. In addition, since our bivalent EIV LAIV is based on the use of reverse genetics approaches, our results demonstrate the feasibility of using the backbone of clade 1 Ohio/03 LAIV as a master donor virus (MDV) for the production and rapid update of LAIVs for the control and protection against other EIV strains of epidemiological relevance to horses.This research was partially funded by the University of Rochester Technology Development Funding to L.M.-S. and T.M.C., P.R.M. was funded by the Medical Research Council of the United Kingdom (Grant MC_UU_120/14/9). T.M.C. and S.R. were supported by the Kentucky Agricultural Experiment Station (Project no. KY014053) and by a pilot study funding from the College of Agriculture, Food, and the Environment at University of Kentucky. F.S.O. was supported by a Young Investigator Award from the American Quarter Horse Foundation, and by the Geoffrey C. Hughes Foundation.Peer reviewedMultidisciplinary Digital Publishing InstituteUniversity of RochesterMedical Research Council (UK)University of KentuckyBlanco-Lobo, Pilar [0000-0003-1893-8198]Reedy, Stephanie [0000-0002-7947-0990]Oladunni, Fatai S [0000-0001-5050-0183]Nogales, Aitor [0000-0002-2424-7900]Murcia, Pablo R. [0000-0002-4352-394X]Martínez-Sobrido, Luis [0000-0001-7084-0804]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/362376https://api.elsevier.com/content/abstract/scopus_id/85073419500reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésCentro de Investigación en Sanidad Animal (CISA)https://doi.org/10.3390/v11100933Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3623762026-05-22T06:33:51Z
dc.title.none.fl_str_mv A Bivalent Live-Attenuated Vaccine for the Prevention of Equine Influenza Virus
title A Bivalent Live-Attenuated Vaccine for the Prevention of Equine Influenza Virus
spellingShingle A Bivalent Live-Attenuated Vaccine for the Prevention of Equine Influenza Virus
Blanco-Lobo, Pilar
Equine influenza virus
Live-attenuated influenza vaccine
Master donor virus
Recombinant virus
Reverse genetics techniques
title_short A Bivalent Live-Attenuated Vaccine for the Prevention of Equine Influenza Virus
title_full A Bivalent Live-Attenuated Vaccine for the Prevention of Equine Influenza Virus
title_fullStr A Bivalent Live-Attenuated Vaccine for the Prevention of Equine Influenza Virus
title_full_unstemmed A Bivalent Live-Attenuated Vaccine for the Prevention of Equine Influenza Virus
title_sort A Bivalent Live-Attenuated Vaccine for the Prevention of Equine Influenza Virus
dc.creator.none.fl_str_mv Blanco-Lobo, Pilar
Rodriguez, Laura
Reedy, Stephanie
Oladunni, Fatai S.
Nogales, Aitor
Murcia, Pablo R.
Chambers, Thomas M.
Martínez-Sobrido, Luis
author Blanco-Lobo, Pilar
author_facet Blanco-Lobo, Pilar
Rodriguez, Laura
Reedy, Stephanie
Oladunni, Fatai S.
Nogales, Aitor
Murcia, Pablo R.
Chambers, Thomas M.
Martínez-Sobrido, Luis
author_role author
author2 Rodriguez, Laura
Reedy, Stephanie
Oladunni, Fatai S.
Nogales, Aitor
Murcia, Pablo R.
Chambers, Thomas M.
Martínez-Sobrido, Luis
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv University of Rochester
Medical Research Council (UK)
University of Kentucky
Blanco-Lobo, Pilar [0000-0003-1893-8198]
Reedy, Stephanie [0000-0002-7947-0990]
Oladunni, Fatai S [0000-0001-5050-0183]
Nogales, Aitor [0000-0002-2424-7900]
Murcia, Pablo R. [0000-0002-4352-394X]
Martínez-Sobrido, Luis [0000-0001-7084-0804]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Equine influenza virus
Live-attenuated influenza vaccine
Master donor virus
Recombinant virus
Reverse genetics techniques
topic Equine influenza virus
Live-attenuated influenza vaccine
Master donor virus
Recombinant virus
Reverse genetics techniques
description 22 Pág.
publishDate 2019
dc.date.none.fl_str_mv 2019
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/362376
https://api.elsevier.com/content/abstract/scopus_id/85073419500
url http://hdl.handle.net/10261/362376
https://api.elsevier.com/content/abstract/scopus_id/85073419500
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Centro de Investigación en Sanidad Animal (CISA)
https://doi.org/10.3390/v11100933
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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