Amperometric determination of endoglin in human serum using disposable immunosensors constructed with poly(pyrrolepropionic) acid-modified electrodes

24 p.-6 fig.-2 tab.

Bibliographic Details
Authors: Martínez-Periñán, Emiliano, Sánchez-Tirado, E., González-Cortés, Araceli, Barderas, Rodrigo, Sánchez-Puelles, José María, Martínez-Santamaría, Lucía, Campuzano, Susana, Yáñez-Sedeño, Pilar, Pingarrón, José Manuel
Format: article
Status:Versión aceptada para publicación
Publication Date:2018
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/171261
Online Access:http://hdl.handle.net/10261/171261
Access Level:Open access
Keyword:Endoglin
CD105
Human serum
Electrochemical immunosensor,
Serum
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spelling Amperometric determination of endoglin in human serum using disposable immunosensors constructed with poly(pyrrolepropionic) acid-modified electrodesMartínez-Periñán, EmilianoSánchez-Tirado, E.González-Cortés, AraceliBarderas, RodrigoSánchez-Puelles, José MaríaMartínez-Santamaría, LucíaCampuzano, SusanaYáñez-Sedeño, PilarPingarrón, José ManuelEndoglinCD105Human serumElectrochemical immunosensor,Serum24 p.-6 fig.-2 tab.An amperometric immunosensor for the determination of the biomarker endoglin (CD105) to comply with the requirements of sensitivity and accuracy demanded in clinical practice is reported in this work. The immunosensing platform is implemented onto disposable electrodes modified with poly(pyrrolepropionic) acid (pPPA). The methodology involves a sandwich configuration and labeling of the biotinylated detector antibody with poly-HRP-streptavidin for signal amplification. Amperometric detection of hydrogen peroxide reduction in the presence of HQ was employed as analytical readout. The different steps involved in the immunosensor preparation were monitored by electrochemical impedance spectroscopy. The resulting immunosensor provided a linear range between 0.18 and 20 ng mL-1, adequate for the determination of CD105 in serum, with a detection limit (LOD) of 140 pg mL−1. These analytical characteristics improve those reported previously for other electrochemical immunosensors. A comparison with immunosensors prepared using other electrochemical scaffolds for anti-CD105 immobilization involving carboxylated doubled walled carbon nanotubes or modification by grafting, showed that a better performance was achieved using pPPA-modified SPCEs. A good reproducibility of the measurements, an excellent storage stability of the anti-CD105-pPPA/SPCE bioplatforms and an excellent selectivity of the resulting immunosensors were found. The usefulness of the immunosensors was tested by analyzing human serum samples collected from healthy individuals and patients of colorectal, breast and lung cancer and epidermolysis bullosa. The results were successfully validated against those provided by an ELISA kit.The financial support of the Spanish Ministerio de Economía y Competitividad CTQ2015-70023-R and CTQ2015-64402-C2-1-R, and SAF2014-53209-R Research Projects, the PI17CIII/00045 research project from AESI and the NANOAVANSENS Program from the Comunidad de Madrid (S2013/MT-3029).Peer reviewedElsevierMinisterio de Economía y Competitividad (España)Comunidad de MadridMartínez-Periñán, E. [0000-0003-3122-3381]Sánchez-Tirado, E. [0000-0002-2386-4552]González-Cortés, A. [0000-0001-9054-9993]Barderas, Rodrigo [0000-0003-3539-7469]Sánchez-Puelles, José María [0000-0002-4283-4885]Campuzano, Susana [0000-0002-9928-6613]Pingarrón, J.M. [0000-0003-2271-1383]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201820182018info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/171261reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2015-70023-Rinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2015-64402-C2-1-Rinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SAF2014-53209-RS2013/MT-3029/NANOAVANSENShttps://doi.org/10.1016/j.electacta.2018.10.015Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1712612026-05-22T06:33:51Z
dc.title.none.fl_str_mv Amperometric determination of endoglin in human serum using disposable immunosensors constructed with poly(pyrrolepropionic) acid-modified electrodes
title Amperometric determination of endoglin in human serum using disposable immunosensors constructed with poly(pyrrolepropionic) acid-modified electrodes
spellingShingle Amperometric determination of endoglin in human serum using disposable immunosensors constructed with poly(pyrrolepropionic) acid-modified electrodes
Martínez-Periñán, Emiliano
Endoglin
CD105
Human serum
Electrochemical immunosensor,
Serum
title_short Amperometric determination of endoglin in human serum using disposable immunosensors constructed with poly(pyrrolepropionic) acid-modified electrodes
title_full Amperometric determination of endoglin in human serum using disposable immunosensors constructed with poly(pyrrolepropionic) acid-modified electrodes
title_fullStr Amperometric determination of endoglin in human serum using disposable immunosensors constructed with poly(pyrrolepropionic) acid-modified electrodes
title_full_unstemmed Amperometric determination of endoglin in human serum using disposable immunosensors constructed with poly(pyrrolepropionic) acid-modified electrodes
title_sort Amperometric determination of endoglin in human serum using disposable immunosensors constructed with poly(pyrrolepropionic) acid-modified electrodes
dc.creator.none.fl_str_mv Martínez-Periñán, Emiliano
Sánchez-Tirado, E.
González-Cortés, Araceli
Barderas, Rodrigo
Sánchez-Puelles, José María
Martínez-Santamaría, Lucía
Campuzano, Susana
Yáñez-Sedeño, Pilar
Pingarrón, José Manuel
author Martínez-Periñán, Emiliano
author_facet Martínez-Periñán, Emiliano
Sánchez-Tirado, E.
González-Cortés, Araceli
Barderas, Rodrigo
Sánchez-Puelles, José María
Martínez-Santamaría, Lucía
Campuzano, Susana
Yáñez-Sedeño, Pilar
Pingarrón, José Manuel
author_role author
author2 Sánchez-Tirado, E.
González-Cortés, Araceli
Barderas, Rodrigo
Sánchez-Puelles, José María
Martínez-Santamaría, Lucía
Campuzano, Susana
Yáñez-Sedeño, Pilar
Pingarrón, José Manuel
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Comunidad de Madrid
Martínez-Periñán, E. [0000-0003-3122-3381]
Sánchez-Tirado, E. [0000-0002-2386-4552]
González-Cortés, A. [0000-0001-9054-9993]
Barderas, Rodrigo [0000-0003-3539-7469]
Sánchez-Puelles, José María [0000-0002-4283-4885]
Campuzano, Susana [0000-0002-9928-6613]
Pingarrón, J.M. [0000-0003-2271-1383]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Endoglin
CD105
Human serum
Electrochemical immunosensor,
Serum
topic Endoglin
CD105
Human serum
Electrochemical immunosensor,
Serum
description 24 p.-6 fig.-2 tab.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/171261
url http://hdl.handle.net/10261/171261
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2015-70023-R
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2015-64402-C2-1-R
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SAF2014-53209-R
S2013/MT-3029/NANOAVANSENS
https://doi.org/10.1016/j.electacta.2018.10.015
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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