Recurrent Immunogenic Neoantigens and Their Cognate T-cell Receptors in Treatment-Resistant Metastatic Prostate Cancer

New approaches that generate long-lasting therapeutic responses in patients with therapy-resistant metastatic cancer are urgently needed. To address this challenge, we developed Spot Neoantigens in Metastases (SpotNeoMet), a novel data-driven pipeline that systematically identifies recurrently prese...

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Detalles Bibliográficos
Autores: Gumpert, Nofar, Sagie, Shira, Arnedo Pac, Claudia, Babu, Tomer, Weller, Chen, Gonzalez Perez, Abel David, Wang, Yuan, Todó, Lucas Michel, Levy, Ronen, Chen, Xi, Greenberg, Polina, Dayan-Rubinov, Maria, Yakubovich, Elizabeta, Wasserman-Bartov, Talya, Zerbib, Mirie, Gong, Jianhui, Rebernick, Ryan J., Oliveira Tercero, Anna, Agundez Muriel, Laura, Benedek, Gil, Kedmi, Merav, Oren, Roni, Ben-Dor, Shifra, Levin, Yishai, Troyanskaya, Olga G., Munzur, Aslı D., Wyatt, Alexander W., Cieslik, Marcin P., Quigley, David A., Van Allen, Eliezer M., Anandasabapathy, Niroshana, Mateo, Joaquin, Yang, Xinbo, Martínez Jiménez, Francisco, López Bigas, Núria, Samuels, Yardena
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2026
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/227763
Acceso en línea:https://hdl.handle.net/2445/227763
Access Level:acceso abierto
Palabra clave:Metàstasi
Proteïnes supressores de tumors
Càncer d'úter
Metastasis
Tumor suppressor protein
Uterine cancer
Descripción
Sumario:New approaches that generate long-lasting therapeutic responses in patients with therapy-resistant metastatic cancer are urgently needed. To address this challenge, we developed Spot Neoantigens in Metastases (SpotNeoMet), a novel data-driven pipeline that systematically identifies recurrently presented neopeptides in treatment-resistant patients. We identified seven therapy resistance mutations predicted to produce neopeptides presented by common HLAs. Using HLA immunopeptidomics, we discovered three novel neopeptides derived from androgen receptor (AR) H875Y, a common metastatic castration-resistant prostate cancer (mCRPC) mutation. We validated these neoantigens as highly immunogenic and then isolated and characterized cognate T-cell receptors (TCR) from healthy donor peripheral blood mononuclear cells. We demonstrated that AR H875Y-specific TCRs are highly specific and kill prostate cancer cells presenting AR neopeptides in vitro and in vivo. Our new pipeline identifies novel immunotherapy targets and potential treatment options for patients with mCRPC. Moreover, SpotNeoMet offers a systematic route to identify "HLA-peptide" pairs and their cognate TCRs across treatment-resistant cancers.Significance: As the emergence of resistance to targeted treatments in patients with metastatic cancer, there is an urgent need for innovative therapeutic approaches for this population. Our study provides a new analytic framework to identify neoantigens from treatment-resistant mutations and a proof-of-concept T cell-based immunotherapy treatment for mCRPC.

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